Isolation and identification of potentially pathogenic free-living amoeba in drinking, surface, and stagnant water sources from Alborz Province, Iran
Free-living amoebas (FLAs) can cause neurological and ocular complications in humans. Water supplies play a critical role in transmitting FLAs to humans. The aim of the present study was to investigate the presence of FLAs in various aquatic sources including drinking water, stagnant water, and surf...
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| Format: | Article |
| Language: | English |
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IWA Publishing
2022-04-01
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| Series: | Journal of Water and Health |
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| Online Access: | http://jwh.iwaponline.com/content/20/4/620 |
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| author | Ehsan Javanmard Maryam Niyyati Ali Taghipour Marziye Fatemi Hamed Mirjalali Panagiotis Karanis |
| author_facet | Ehsan Javanmard Maryam Niyyati Ali Taghipour Marziye Fatemi Hamed Mirjalali Panagiotis Karanis |
| author_sort | Ehsan Javanmard |
| collection | DOAJ |
| description | Free-living amoebas (FLAs) can cause neurological and ocular complications in humans. Water supplies play a critical role in transmitting FLAs to humans. The aim of the present study was to investigate the presence of FLAs in various aquatic sources including drinking water, stagnant water, and surface water in Alborz province, northern Iran, using morphological and molecular techniques. A total of 70 water samples were collected from 34 drinking waters, 23 surface waters, and 13 stagnant waters. Filtration and cultivation were employed to isolate FLAs. PCR assay was applied by using the genus-specific primers on positive samples. Pathogenicity tests (osmo- and thermo-tolerance properties) were performed for Acanthamoeba spp., positive sample. Considering the morphological criteria, four positive samples of Acanthamoeba sp., three Vermamoeba sp., two mixed Vermamoeba sp. with Vahlkamfiids, and one mixed Acanthamoeba sp. with Vahlkamfiids were isolated. Five Acanthamoeba sp. isolates were amplified using the JDP primer pairs. Among them, two genotypes, T4 (three isolates) and T5 (two isolates) corresponding to A. lenticulata, were identified. Four V. vermiformis samples were confirmed using the sequencing. This study highlighted the occurrence of potentially pathogenic waterborne FLAs in water habitats associated with high human activity. The results of such research on the prevalence of FLAs, as a human hazard, should be communicated to health policymakers. HIGHLIGHTS
Water samples were collected from drinking, surface, and stagnant waters.;
According to the morphological assessment, Acanthamoeba, Vermamoeba, and Vahlkamfiids were detected among samples.;
The genotypes T4 and T5 (corresponding to A. lenticulata) were characterized.; |
| first_indexed | 2024-04-14T07:03:36Z |
| format | Article |
| id | doaj.art-5e4233fc42304b8f838433e13a3e5a54 |
| institution | Directory Open Access Journal |
| issn | 1477-8920 1996-7829 |
| language | English |
| last_indexed | 2024-04-14T07:03:36Z |
| publishDate | 2022-04-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Journal of Water and Health |
| spelling | doaj.art-5e4233fc42304b8f838433e13a3e5a542022-12-22T02:06:40ZengIWA PublishingJournal of Water and Health1477-89201996-78292022-04-0120462062910.2166/wh.2022.229229Isolation and identification of potentially pathogenic free-living amoeba in drinking, surface, and stagnant water sources from Alborz Province, IranEhsan Javanmard0Maryam Niyyati1Ali Taghipour2Marziye Fatemi3Hamed Mirjalali4Panagiotis Karanis5 Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany Free-living amoebas (FLAs) can cause neurological and ocular complications in humans. Water supplies play a critical role in transmitting FLAs to humans. The aim of the present study was to investigate the presence of FLAs in various aquatic sources including drinking water, stagnant water, and surface water in Alborz province, northern Iran, using morphological and molecular techniques. A total of 70 water samples were collected from 34 drinking waters, 23 surface waters, and 13 stagnant waters. Filtration and cultivation were employed to isolate FLAs. PCR assay was applied by using the genus-specific primers on positive samples. Pathogenicity tests (osmo- and thermo-tolerance properties) were performed for Acanthamoeba spp., positive sample. Considering the morphological criteria, four positive samples of Acanthamoeba sp., three Vermamoeba sp., two mixed Vermamoeba sp. with Vahlkamfiids, and one mixed Acanthamoeba sp. with Vahlkamfiids were isolated. Five Acanthamoeba sp. isolates were amplified using the JDP primer pairs. Among them, two genotypes, T4 (three isolates) and T5 (two isolates) corresponding to A. lenticulata, were identified. Four V. vermiformis samples were confirmed using the sequencing. This study highlighted the occurrence of potentially pathogenic waterborne FLAs in water habitats associated with high human activity. The results of such research on the prevalence of FLAs, as a human hazard, should be communicated to health policymakers. HIGHLIGHTS Water samples were collected from drinking, surface, and stagnant waters.; According to the morphological assessment, Acanthamoeba, Vermamoeba, and Vahlkamfiids were detected among samples.; The genotypes T4 and T5 (corresponding to A. lenticulata) were characterized.;http://jwh.iwaponline.com/content/20/4/620free-living amoebairanpathogenicitywater sources |
| spellingShingle | Ehsan Javanmard Maryam Niyyati Ali Taghipour Marziye Fatemi Hamed Mirjalali Panagiotis Karanis Isolation and identification of potentially pathogenic free-living amoeba in drinking, surface, and stagnant water sources from Alborz Province, Iran Journal of Water and Health free-living amoeba iran pathogenicity water sources |
| title | Isolation and identification of potentially pathogenic free-living amoeba in drinking, surface, and stagnant water sources from Alborz Province, Iran |
| title_full | Isolation and identification of potentially pathogenic free-living amoeba in drinking, surface, and stagnant water sources from Alborz Province, Iran |
| title_fullStr | Isolation and identification of potentially pathogenic free-living amoeba in drinking, surface, and stagnant water sources from Alborz Province, Iran |
| title_full_unstemmed | Isolation and identification of potentially pathogenic free-living amoeba in drinking, surface, and stagnant water sources from Alborz Province, Iran |
| title_short | Isolation and identification of potentially pathogenic free-living amoeba in drinking, surface, and stagnant water sources from Alborz Province, Iran |
| title_sort | isolation and identification of potentially pathogenic free living amoeba in drinking surface and stagnant water sources from alborz province iran |
| topic | free-living amoeba iran pathogenicity water sources |
| url | http://jwh.iwaponline.com/content/20/4/620 |
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