Comparison of Antibiotic Resistance Profile and Biofilm Production of <i>Staphylococcus aureus</i> Isolates Derived from Human Specimens and Animal-Derived Samples
Background: The diffusion of antimicrobial resistance is a significant concern for public health worldwide. <i>Staphylococcus aureus</i> represents a paradigm microorganism for antibiotic resistance in that resistant strains appear within a decade after the introduction of new antibiotic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/8/3/97 |
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| author | Maria Vitale Paola Galluzzo Patrizia Giuseppina Buffa Eleonora Carlino Orazio Spezia Rosa Alduina |
| author_facet | Maria Vitale Paola Galluzzo Patrizia Giuseppina Buffa Eleonora Carlino Orazio Spezia Rosa Alduina |
| author_sort | Maria Vitale |
| collection | DOAJ |
| description | Background: The diffusion of antimicrobial resistance is a significant concern for public health worldwide. <i>Staphylococcus aureus</i> represents a paradigm microorganism for antibiotic resistance in that resistant strains appear within a decade after the introduction of new antibiotics. Methods: Fourteen <i>S. aureus</i> isolates from human specimens and twenty-one from samples of animal origin, were compared for their antimicrobial resistance and biofilm capability. In addition, they were characterized at the molecular level to detect the antimicrobial resistance <i>mecA</i> gene and genes related with enterotoxin, toxin, and biofilm production. Results: Both phenotypic and molecular analysis showed main differences among human- and animal-derived isolates. Among the human-derived isolates, more multidrug-resistant isolates were detected and <i>mecA</i> gene, enterotoxin, and toxin genes were more prevalent. Different genes involved in biofilm production were detected with <i>bap</i> present only in animal-derived isolates and <i>sasC</i> present in both isolates, however, with a higher prevalence in the human-derived isolates. Biofilm capability was higher in human-derived isolates mainly associated to the <i>sasC</i> gene. Conclusions: The overall results indicate that human <i>S. aureus</i> isolates are more virulent and resistant than the isolates of animal origin randomly selected with no infection anamnesis. This study confirms that selection for more virulent and resistant <i>S. aureus</i> strains is related to the clinical practice. |
| first_indexed | 2024-04-13T00:15:46Z |
| format | Article |
| id | doaj.art-5e425691ca2149f48f654e9217bf1b13 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-04-13T00:15:46Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-5e425691ca2149f48f654e9217bf1b132022-12-22T03:10:56ZengMDPI AGAntibiotics2079-63822019-07-01839710.3390/antibiotics8030097antibiotics8030097Comparison of Antibiotic Resistance Profile and Biofilm Production of <i>Staphylococcus aureus</i> Isolates Derived from Human Specimens and Animal-Derived SamplesMaria Vitale0Paola Galluzzo1Patrizia Giuseppina Buffa2Eleonora Carlino3Orazio Spezia4Rosa Alduina5Laboratorio Genetica dei Microorganismi, Istituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyLaboratorio Genetica dei Microorganismi, Istituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyDipartimento Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Viale delle Scienze, University of Palermo, 90128 Palermo, ItalyLaboratorio Genetica dei Microorganismi, Istituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyLaboratorio Analisi Baiata srl, via Capitano Francesco Sieli, 1, 91100 Trapani (TP), ItalyDipartimento Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Viale delle Scienze, University of Palermo, 90128 Palermo, ItalyBackground: The diffusion of antimicrobial resistance is a significant concern for public health worldwide. <i>Staphylococcus aureus</i> represents a paradigm microorganism for antibiotic resistance in that resistant strains appear within a decade after the introduction of new antibiotics. Methods: Fourteen <i>S. aureus</i> isolates from human specimens and twenty-one from samples of animal origin, were compared for their antimicrobial resistance and biofilm capability. In addition, they were characterized at the molecular level to detect the antimicrobial resistance <i>mecA</i> gene and genes related with enterotoxin, toxin, and biofilm production. Results: Both phenotypic and molecular analysis showed main differences among human- and animal-derived isolates. Among the human-derived isolates, more multidrug-resistant isolates were detected and <i>mecA</i> gene, enterotoxin, and toxin genes were more prevalent. Different genes involved in biofilm production were detected with <i>bap</i> present only in animal-derived isolates and <i>sasC</i> present in both isolates, however, with a higher prevalence in the human-derived isolates. Biofilm capability was higher in human-derived isolates mainly associated to the <i>sasC</i> gene. Conclusions: The overall results indicate that human <i>S. aureus</i> isolates are more virulent and resistant than the isolates of animal origin randomly selected with no infection anamnesis. This study confirms that selection for more virulent and resistant <i>S. aureus</i> strains is related to the clinical practice.https://www.mdpi.com/2079-6382/8/3/97<i>Staphylococcus aureus</i>Staphylococcal toxinsmecAantibiotic resistancebiofilm activityMRSA |
| spellingShingle | Maria Vitale Paola Galluzzo Patrizia Giuseppina Buffa Eleonora Carlino Orazio Spezia Rosa Alduina Comparison of Antibiotic Resistance Profile and Biofilm Production of <i>Staphylococcus aureus</i> Isolates Derived from Human Specimens and Animal-Derived Samples Antibiotics <i>Staphylococcus aureus</i> Staphylococcal toxins mecA antibiotic resistance biofilm activity MRSA |
| title | Comparison of Antibiotic Resistance Profile and Biofilm Production of <i>Staphylococcus aureus</i> Isolates Derived from Human Specimens and Animal-Derived Samples |
| title_full | Comparison of Antibiotic Resistance Profile and Biofilm Production of <i>Staphylococcus aureus</i> Isolates Derived from Human Specimens and Animal-Derived Samples |
| title_fullStr | Comparison of Antibiotic Resistance Profile and Biofilm Production of <i>Staphylococcus aureus</i> Isolates Derived from Human Specimens and Animal-Derived Samples |
| title_full_unstemmed | Comparison of Antibiotic Resistance Profile and Biofilm Production of <i>Staphylococcus aureus</i> Isolates Derived from Human Specimens and Animal-Derived Samples |
| title_short | Comparison of Antibiotic Resistance Profile and Biofilm Production of <i>Staphylococcus aureus</i> Isolates Derived from Human Specimens and Animal-Derived Samples |
| title_sort | comparison of antibiotic resistance profile and biofilm production of i staphylococcus aureus i isolates derived from human specimens and animal derived samples |
| topic | <i>Staphylococcus aureus</i> Staphylococcal toxins mecA antibiotic resistance biofilm activity MRSA |
| url | https://www.mdpi.com/2079-6382/8/3/97 |
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