Genome-Wide Identification of <i>LBD</i> Genes in Foxtail Millet (<i>Setaria italica</i>) and Functional Characterization of <i>SiLBD21</i>
Plant-specific lateral organ boundaries domain (LBD) proteins play important roles in plant growth and development. Foxtail millet (<i>Setaria italica</i>) is one new C<sub>4</sub> model crop. However, the functions of foxtail millet <i>LBD</i> genes are unknown....
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/8/7110 |
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| author | Kunjie Li Yaning Wei Yimin Wang Bin Tan Shoukun Chen Haifeng Li |
| author_facet | Kunjie Li Yaning Wei Yimin Wang Bin Tan Shoukun Chen Haifeng Li |
| author_sort | Kunjie Li |
| collection | DOAJ |
| description | Plant-specific lateral organ boundaries domain (LBD) proteins play important roles in plant growth and development. Foxtail millet (<i>Setaria italica</i>) is one new C<sub>4</sub> model crop. However, the functions of foxtail millet <i>LBD</i> genes are unknown. In this study, a genome-wide identification of foxtail millet <i>LBD</i> genes and a systematical analysis were conducted. A total of 33 <i>SiLBD</i> genes were identified. They are unevenly distributed on nine chromosomes. Among these <i>SiLBD</i> genes, six segmental duplication pairs were detected. The thirty-three encoded SiLBD proteins could be classified into two classes and seven clades. Members in the same clade have similar gene structure and motif composition. Forty-seven kinds of <i>cis</i>-elements were found in the putative promoters, and they are related to development/growth, hormone, and abiotic stress response, respectively. Meanwhile, the expression pattern was investigated. Most <i>SiLBD</i> genes are expressed in different tissues, while several genes are mainly expressed in one or two kinds of tissues. In addition, most <i>SiLBD</i> genes respond to different abiotic stresses. Furthermore, the function of <i>SiLBD21</i>, which is mainly expressed in roots, was characterized by ectopic expression in <i>Arabidopsis</i> and rice. Compared to controls, transgenic plants generated shorter primary roots and more lateral roots, indicating the function of <i>SiLBD21</i> in root development. Overall, our study laid the foundation for further functional elucidation of <i>SiLBD</i> genes. |
| first_indexed | 2024-03-11T04:57:03Z |
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| language | English |
| last_indexed | 2024-03-11T04:57:03Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5e45a9b46fe64a5a9e78f187808cd4112023-11-17T19:35:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01248711010.3390/ijms24087110Genome-Wide Identification of <i>LBD</i> Genes in Foxtail Millet (<i>Setaria italica</i>) and Functional Characterization of <i>SiLBD21</i>Kunjie Li0Yaning Wei1Yimin Wang2Bin Tan3Shoukun Chen4Haifeng Li5College of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, ChinaCollege of Agronomy, Northwest A&F University, Yangling, Xianyang 712100, ChinaPlant-specific lateral organ boundaries domain (LBD) proteins play important roles in plant growth and development. Foxtail millet (<i>Setaria italica</i>) is one new C<sub>4</sub> model crop. However, the functions of foxtail millet <i>LBD</i> genes are unknown. In this study, a genome-wide identification of foxtail millet <i>LBD</i> genes and a systematical analysis were conducted. A total of 33 <i>SiLBD</i> genes were identified. They are unevenly distributed on nine chromosomes. Among these <i>SiLBD</i> genes, six segmental duplication pairs were detected. The thirty-three encoded SiLBD proteins could be classified into two classes and seven clades. Members in the same clade have similar gene structure and motif composition. Forty-seven kinds of <i>cis</i>-elements were found in the putative promoters, and they are related to development/growth, hormone, and abiotic stress response, respectively. Meanwhile, the expression pattern was investigated. Most <i>SiLBD</i> genes are expressed in different tissues, while several genes are mainly expressed in one or two kinds of tissues. In addition, most <i>SiLBD</i> genes respond to different abiotic stresses. Furthermore, the function of <i>SiLBD21</i>, which is mainly expressed in roots, was characterized by ectopic expression in <i>Arabidopsis</i> and rice. Compared to controls, transgenic plants generated shorter primary roots and more lateral roots, indicating the function of <i>SiLBD21</i> in root development. Overall, our study laid the foundation for further functional elucidation of <i>SiLBD</i> genes.https://www.mdpi.com/1422-0067/24/8/7110<i>LBD</i>foxtail milletroot developmentover-expressionfunctional characterization |
| spellingShingle | Kunjie Li Yaning Wei Yimin Wang Bin Tan Shoukun Chen Haifeng Li Genome-Wide Identification of <i>LBD</i> Genes in Foxtail Millet (<i>Setaria italica</i>) and Functional Characterization of <i>SiLBD21</i> International Journal of Molecular Sciences <i>LBD</i> foxtail millet root development over-expression functional characterization |
| title | Genome-Wide Identification of <i>LBD</i> Genes in Foxtail Millet (<i>Setaria italica</i>) and Functional Characterization of <i>SiLBD21</i> |
| title_full | Genome-Wide Identification of <i>LBD</i> Genes in Foxtail Millet (<i>Setaria italica</i>) and Functional Characterization of <i>SiLBD21</i> |
| title_fullStr | Genome-Wide Identification of <i>LBD</i> Genes in Foxtail Millet (<i>Setaria italica</i>) and Functional Characterization of <i>SiLBD21</i> |
| title_full_unstemmed | Genome-Wide Identification of <i>LBD</i> Genes in Foxtail Millet (<i>Setaria italica</i>) and Functional Characterization of <i>SiLBD21</i> |
| title_short | Genome-Wide Identification of <i>LBD</i> Genes in Foxtail Millet (<i>Setaria italica</i>) and Functional Characterization of <i>SiLBD21</i> |
| title_sort | genome wide identification of i lbd i genes in foxtail millet i setaria italica i and functional characterization of i silbd21 i |
| topic | <i>LBD</i> foxtail millet root development over-expression functional characterization |
| url | https://www.mdpi.com/1422-0067/24/8/7110 |
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