Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women
Abstract Background The establishment of symbiotic microbiota in pregnant women is important for both the mother and her offspring. Little is known about the salivary symbiotic bacteria in pregnancy, and analysis of composition of microbiome (ANCOM) is useful to detect small differences in the numbe...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-022-02293-0 |
| _version_ | 1818117186131066880 |
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| author | Satsuki Kato Toshiyuki Nagasawa Osamu Uehara Shintaro Shimizu Nodoka Sugiyama Kozue Hasegawa-Nakamura Kazuyuki Noguchi Masayuki Hatae Hiroshige Kakinoki Yasushi Furuichi |
| author_facet | Satsuki Kato Toshiyuki Nagasawa Osamu Uehara Shintaro Shimizu Nodoka Sugiyama Kozue Hasegawa-Nakamura Kazuyuki Noguchi Masayuki Hatae Hiroshige Kakinoki Yasushi Furuichi |
| author_sort | Satsuki Kato |
| collection | DOAJ |
| description | Abstract Background The establishment of symbiotic microbiota in pregnant women is important for both the mother and her offspring. Little is known about the salivary symbiotic bacteria in pregnancy, and analysis of composition of microbiome (ANCOM) is useful to detect small differences in the number of bacteria. The aim of this study was to investigate the differences in the salivary bacteria between healthy pregnant and non-pregnant women using ANCOM. Methods Unstimulated saliva samples were collected from 35 healthy pregnant women at 35 weeks gestation and 30 healthy non-pregnant women during menstruation. All participants underwent a periodontal examination. Estradiol and progesterone levels were examined by enzyme-linked immunosorbent assay. DNA extracted from the saliva was assessed by 16S ribosomal RNA amplicon sequencing and real-time PCR. Results Salivary estradiol and progesterone levels were significantly increased in pregnant women. The alpha and beta diversities were higher in pregnant women than in non-pregnant women. The largest effect size difference noted when the microbiota of the pregnant and non-pregnant women were analyzed was that for Bifidobacteriales. Levels of Bifidobacterium dentium, but not of Bifidobacterium adolescentis, were significantly increased in pregnant women, and the levels were significantly correlated with progesterone concentration. Conclusion The results suggest that Bifidobacterium and progesterone levels are elevated in the saliva of healthy pregnant women compared with non-pregnant women. |
| first_indexed | 2024-12-11T04:34:24Z |
| format | Article |
| id | doaj.art-5e46754b4f4a49c8932e5a99b1f4f62c |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-12-11T04:34:24Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-5e46754b4f4a49c8932e5a99b1f4f62c2022-12-22T01:20:47ZengBMCBMC Oral Health1472-68312022-06-0122111310.1186/s12903-022-02293-0Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant womenSatsuki Kato0Toshiyuki Nagasawa1Osamu Uehara2Shintaro Shimizu3Nodoka Sugiyama4Kozue Hasegawa-Nakamura5Kazuyuki Noguchi6Masayuki Hatae7Hiroshige Kakinoki8Yasushi Furuichi9Division of Periodontology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of HokkaidoDivision of Advanced Clinical Education, Department of Integrated Dental Education, School of Dentistry, Health Sciences University of HokkaidoDivision of Disease Control and Molecular Epidemiology, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of HokkaidoDivision of Periodontology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of HokkaidoDivision of Periodontology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of HokkaidoDepartment of Periodontology, Field of Oral and Maxillofacial Rehabilitation, Advanced Therapeutic Course, Kagoshima University Graduate School of Medical and Dental SciencesDepartment of Periodontology, Field of Oral and Maxillofacial Rehabilitation, Advanced Therapeutic Course, Kagoshima University Graduate School of Medical and Dental SciencesKakinoki HospitalKakinoki HospitalDivision of Periodontology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of HokkaidoAbstract Background The establishment of symbiotic microbiota in pregnant women is important for both the mother and her offspring. Little is known about the salivary symbiotic bacteria in pregnancy, and analysis of composition of microbiome (ANCOM) is useful to detect small differences in the number of bacteria. The aim of this study was to investigate the differences in the salivary bacteria between healthy pregnant and non-pregnant women using ANCOM. Methods Unstimulated saliva samples were collected from 35 healthy pregnant women at 35 weeks gestation and 30 healthy non-pregnant women during menstruation. All participants underwent a periodontal examination. Estradiol and progesterone levels were examined by enzyme-linked immunosorbent assay. DNA extracted from the saliva was assessed by 16S ribosomal RNA amplicon sequencing and real-time PCR. Results Salivary estradiol and progesterone levels were significantly increased in pregnant women. The alpha and beta diversities were higher in pregnant women than in non-pregnant women. The largest effect size difference noted when the microbiota of the pregnant and non-pregnant women were analyzed was that for Bifidobacteriales. Levels of Bifidobacterium dentium, but not of Bifidobacterium adolescentis, were significantly increased in pregnant women, and the levels were significantly correlated with progesterone concentration. Conclusion The results suggest that Bifidobacterium and progesterone levels are elevated in the saliva of healthy pregnant women compared with non-pregnant women.https://doi.org/10.1186/s12903-022-02293-0PregnancySalivary microbiotaEstradiolProgesteroneBifidobacterium |
| spellingShingle | Satsuki Kato Toshiyuki Nagasawa Osamu Uehara Shintaro Shimizu Nodoka Sugiyama Kozue Hasegawa-Nakamura Kazuyuki Noguchi Masayuki Hatae Hiroshige Kakinoki Yasushi Furuichi Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women BMC Oral Health Pregnancy Salivary microbiota Estradiol Progesterone Bifidobacterium |
| title | Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women |
| title_full | Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women |
| title_fullStr | Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women |
| title_full_unstemmed | Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women |
| title_short | Increase in Bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non-pregnant women |
| title_sort | increase in bifidobacterium is a characteristic of the difference in the salivary microbiota of pregnant and non pregnant women |
| topic | Pregnancy Salivary microbiota Estradiol Progesterone Bifidobacterium |
| url | https://doi.org/10.1186/s12903-022-02293-0 |
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