Immature monocyte derived dendritic cells gene expression profile in response to Virus-Like Particles stimulation
<p>Abstract</p> <p>We have recently developed a candidate HIV-1 vaccine model based on HIV-1 Pr55gag Virus-Like Particles (HIV-VLPs), produced in a baculovirus expression system and presenting a gp120 molecule from an Ugandan HIV-1 isolate of the clade A (HIV-VLP<sub>A</su...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2005-12-01
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| Series: | Journal of Translational Medicine |
| Online Access: | http://www.translational-medicine.com/content/3/1/45 |
| _version_ | 1818057560390893568 |
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| author | Marincola Francesco M Lewis George K Tagliamonte Maria Tornesello Maria Wang Ena Aricò Eleonora Buonaguro Franco M Buonaguro Luigi |
| author_facet | Marincola Francesco M Lewis George K Tagliamonte Maria Tornesello Maria Wang Ena Aricò Eleonora Buonaguro Franco M Buonaguro Luigi |
| author_sort | Marincola Francesco M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>We have recently developed a candidate HIV-1 vaccine model based on HIV-1 Pr55gag Virus-Like Particles (HIV-VLPs), produced in a baculovirus expression system and presenting a gp120 molecule from an Ugandan HIV-1 isolate of the clade A (HIV-VLP<sub>A</sub>s).</p> <p>The HIV-VLP<sub>A</sub>s induce in Balb/c mice systemic and mucosal neutralizing Antibodies as well as cytotoxic T lymphocytes, by intra-peritoneal as well as intra-nasal administration. Moreover, we have recently shown that the baculovirus-expressed HIV-VLPs induce maturation and activation of monocyte-derived dendritic cells (MDDCs) which, in turn, produce Th1- and Th2-specific cytokines and stimulate <it>in vitro </it>a primary and secondary response in autologous CD4+ T cells.</p> <p>In the present manuscript, the effects of the baculovirus-expressed HIV-VLP<sub>A</sub>s on the genomic transcriptional profile of MDDCs obtained from normal healthy donors have been evaluated. The HIV-VLP<sub>A </sub>stimulation, compared to both PBS and LPS treatment, modulate the expression of genes involved in the morphological and functional changes characterizing the MDDCs activation and maturation.</p> <p>The results of gene profiling analysis here presented are highly informative on the global pattern of gene expression alteration underlying the activation of MDDCs by HIV-VLP<sub>A</sub>s at the early stages of the immune response and may be extremely helpful for the identification of exclusive activation markers.</p> |
| first_indexed | 2024-12-10T12:46:41Z |
| format | Article |
| id | doaj.art-5e49842a7d174cff9be62afd290e0c62 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-10T12:46:41Z |
| publishDate | 2005-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-5e49842a7d174cff9be62afd290e0c622022-12-22T01:48:22ZengBMCJournal of Translational Medicine1479-58762005-12-01314510.1186/1479-5876-3-45Immature monocyte derived dendritic cells gene expression profile in response to Virus-Like Particles stimulationMarincola Francesco MLewis George KTagliamonte MariaTornesello MariaWang EnaAricò EleonoraBuonaguro Franco MBuonaguro Luigi<p>Abstract</p> <p>We have recently developed a candidate HIV-1 vaccine model based on HIV-1 Pr55gag Virus-Like Particles (HIV-VLPs), produced in a baculovirus expression system and presenting a gp120 molecule from an Ugandan HIV-1 isolate of the clade A (HIV-VLP<sub>A</sub>s).</p> <p>The HIV-VLP<sub>A</sub>s induce in Balb/c mice systemic and mucosal neutralizing Antibodies as well as cytotoxic T lymphocytes, by intra-peritoneal as well as intra-nasal administration. Moreover, we have recently shown that the baculovirus-expressed HIV-VLPs induce maturation and activation of monocyte-derived dendritic cells (MDDCs) which, in turn, produce Th1- and Th2-specific cytokines and stimulate <it>in vitro </it>a primary and secondary response in autologous CD4+ T cells.</p> <p>In the present manuscript, the effects of the baculovirus-expressed HIV-VLP<sub>A</sub>s on the genomic transcriptional profile of MDDCs obtained from normal healthy donors have been evaluated. The HIV-VLP<sub>A </sub>stimulation, compared to both PBS and LPS treatment, modulate the expression of genes involved in the morphological and functional changes characterizing the MDDCs activation and maturation.</p> <p>The results of gene profiling analysis here presented are highly informative on the global pattern of gene expression alteration underlying the activation of MDDCs by HIV-VLP<sub>A</sub>s at the early stages of the immune response and may be extremely helpful for the identification of exclusive activation markers.</p>http://www.translational-medicine.com/content/3/1/45 |
| spellingShingle | Marincola Francesco M Lewis George K Tagliamonte Maria Tornesello Maria Wang Ena Aricò Eleonora Buonaguro Franco M Buonaguro Luigi Immature monocyte derived dendritic cells gene expression profile in response to Virus-Like Particles stimulation Journal of Translational Medicine |
| title | Immature monocyte derived dendritic cells gene expression profile in response to Virus-Like Particles stimulation |
| title_full | Immature monocyte derived dendritic cells gene expression profile in response to Virus-Like Particles stimulation |
| title_fullStr | Immature monocyte derived dendritic cells gene expression profile in response to Virus-Like Particles stimulation |
| title_full_unstemmed | Immature monocyte derived dendritic cells gene expression profile in response to Virus-Like Particles stimulation |
| title_short | Immature monocyte derived dendritic cells gene expression profile in response to Virus-Like Particles stimulation |
| title_sort | immature monocyte derived dendritic cells gene expression profile in response to virus like particles stimulation |
| url | http://www.translational-medicine.com/content/3/1/45 |
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