Postsynaptic mitochondria are positioned to support functional diversity of dendritic spines
Postsynaptic mitochondria are critical for the development, plasticity, and maintenance of synaptic inputs. However, their relationship to synaptic structure and functional activity is unknown. We examined a correlative dataset from ferret visual cortex with in vivo two-photon calcium imaging of den...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2023-12-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/89682 |
| _version_ | 1797401416619261952 |
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| author | Connon I Thomas Melissa A Ryan Naomi Kamasawa Benjamin Scholl |
| author_facet | Connon I Thomas Melissa A Ryan Naomi Kamasawa Benjamin Scholl |
| author_sort | Connon I Thomas |
| collection | DOAJ |
| description | Postsynaptic mitochondria are critical for the development, plasticity, and maintenance of synaptic inputs. However, their relationship to synaptic structure and functional activity is unknown. We examined a correlative dataset from ferret visual cortex with in vivo two-photon calcium imaging of dendritic spines during visual stimulation and electron microscopy reconstructions of spine ultrastructure, investigating mitochondrial abundance near functionally and structurally characterized spines. Surprisingly, we found no correlation to structural measures of synaptic strength. Instead, we found that mitochondria are positioned near spines with orientation preferences that are dissimilar to the somatic preference. Additionally, we found that mitochondria are positioned near groups of spines with heterogeneous orientation preferences. For a subset of spines with a mitochondrion in the head or neck, synapses were larger and exhibited greater selectivity to visual stimuli than those without a mitochondrion. Our data suggest mitochondria are not necessarily positioned to support the energy needs of strong spines, but rather support the structurally and functionally diverse inputs innervating the basal dendrites of cortical neurons. |
| first_indexed | 2024-03-09T02:09:46Z |
| format | Article |
| id | doaj.art-5e51dd1cd47c477a9e36e813ebb9743f |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-09T02:09:46Z |
| publishDate | 2023-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5e51dd1cd47c477a9e36e813ebb9743f2023-12-07T14:03:19ZengeLife Sciences Publications LtdeLife2050-084X2023-12-011210.7554/eLife.89682Postsynaptic mitochondria are positioned to support functional diversity of dendritic spinesConnon I Thomas0https://orcid.org/0000-0003-0995-9667Melissa A Ryan1https://orcid.org/0000-0002-0468-9525Naomi Kamasawa2https://orcid.org/0000-0002-8926-5309Benjamin Scholl3https://orcid.org/0000-0002-9578-7234Electron Microscopy Core Facility, Max Planck Florida Institute for Neuroscience, Max Planck Way, Jupiter, United StatesElectron Microscopy Core Facility, Max Planck Florida Institute for Neuroscience, Max Planck Way, Jupiter, United StatesElectron Microscopy Core Facility, Max Planck Florida Institute for Neuroscience, Max Planck Way, Jupiter, United StatesDepartment of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United StatesPostsynaptic mitochondria are critical for the development, plasticity, and maintenance of synaptic inputs. However, their relationship to synaptic structure and functional activity is unknown. We examined a correlative dataset from ferret visual cortex with in vivo two-photon calcium imaging of dendritic spines during visual stimulation and electron microscopy reconstructions of spine ultrastructure, investigating mitochondrial abundance near functionally and structurally characterized spines. Surprisingly, we found no correlation to structural measures of synaptic strength. Instead, we found that mitochondria are positioned near spines with orientation preferences that are dissimilar to the somatic preference. Additionally, we found that mitochondria are positioned near groups of spines with heterogeneous orientation preferences. For a subset of spines with a mitochondrion in the head or neck, synapses were larger and exhibited greater selectivity to visual stimuli than those without a mitochondrion. Our data suggest mitochondria are not necessarily positioned to support the energy needs of strong spines, but rather support the structurally and functionally diverse inputs innervating the basal dendrites of cortical neurons.https://elifesciences.org/articles/89682Mustela furoelectron microscopytwo-photon calcium imagingdendrite spinemitochondria |
| spellingShingle | Connon I Thomas Melissa A Ryan Naomi Kamasawa Benjamin Scholl Postsynaptic mitochondria are positioned to support functional diversity of dendritic spines eLife Mustela furo electron microscopy two-photon calcium imaging dendrite spine mitochondria |
| title | Postsynaptic mitochondria are positioned to support functional diversity of dendritic spines |
| title_full | Postsynaptic mitochondria are positioned to support functional diversity of dendritic spines |
| title_fullStr | Postsynaptic mitochondria are positioned to support functional diversity of dendritic spines |
| title_full_unstemmed | Postsynaptic mitochondria are positioned to support functional diversity of dendritic spines |
| title_short | Postsynaptic mitochondria are positioned to support functional diversity of dendritic spines |
| title_sort | postsynaptic mitochondria are positioned to support functional diversity of dendritic spines |
| topic | Mustela furo electron microscopy two-photon calcium imaging dendrite spine mitochondria |
| url | https://elifesciences.org/articles/89682 |
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