Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast.
Polyploidization has crucial impacts on the evolution of different eukaryotic lineages including fungi, plants and animals. Recent genome data suggest that, for many polyploidization events, all duplicated chromosomes are maintained and genome reorganizations occur much later during evolution. Howev...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-11-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC5094715?pdf=render |
| _version_ | 1818700794383302656 |
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| author | Yi-Jin Lu Krishna B S Swamy Jun-Yi Leu |
| author_facet | Yi-Jin Lu Krishna B S Swamy Jun-Yi Leu |
| author_sort | Yi-Jin Lu |
| collection | DOAJ |
| description | Polyploidization has crucial impacts on the evolution of different eukaryotic lineages including fungi, plants and animals. Recent genome data suggest that, for many polyploidization events, all duplicated chromosomes are maintained and genome reorganizations occur much later during evolution. However, newly-formed polyploid genomes are intrinsically unstable and often quickly degenerate into aneuploidy or diploidy. The transition between these two states remains enigmatic. In this study, laboratory evolution experiments were conducted to investigate this phenomenon. We show that robust tetraploidy is achieved in evolved yeast cells by increasing the abundance of Sch9-a protein kinase activated by the TORC1 (Target of Rapamycin Complex 1) and other signaling pathways. Overexpressing SCH9, but not TOR1, allows newly-formed tetraploids to exhibit evolved phenotypes and knocking out SCH9 diminishes the evolved phenotypes. Furthermore, when cells were challenged with conditions causing ancestral cells to evolve aneuploidy, tetraploidy was maintained in the evolved lines. Our results reveal a determinant role for Sch9 during the early stage of polyploid evolution. |
| first_indexed | 2024-12-17T15:10:36Z |
| format | Article |
| id | doaj.art-5e562d6246704991b6ead0753e8d3ea3 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-17T15:10:36Z |
| publishDate | 2016-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-5e562d6246704991b6ead0753e8d3ea32022-12-21T21:43:40ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042016-11-011211e100640910.1371/journal.pgen.1006409Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast.Yi-Jin LuKrishna B S SwamyJun-Yi LeuPolyploidization has crucial impacts on the evolution of different eukaryotic lineages including fungi, plants and animals. Recent genome data suggest that, for many polyploidization events, all duplicated chromosomes are maintained and genome reorganizations occur much later during evolution. However, newly-formed polyploid genomes are intrinsically unstable and often quickly degenerate into aneuploidy or diploidy. The transition between these two states remains enigmatic. In this study, laboratory evolution experiments were conducted to investigate this phenomenon. We show that robust tetraploidy is achieved in evolved yeast cells by increasing the abundance of Sch9-a protein kinase activated by the TORC1 (Target of Rapamycin Complex 1) and other signaling pathways. Overexpressing SCH9, but not TOR1, allows newly-formed tetraploids to exhibit evolved phenotypes and knocking out SCH9 diminishes the evolved phenotypes. Furthermore, when cells were challenged with conditions causing ancestral cells to evolve aneuploidy, tetraploidy was maintained in the evolved lines. Our results reveal a determinant role for Sch9 during the early stage of polyploid evolution.http://europepmc.org/articles/PMC5094715?pdf=render |
| spellingShingle | Yi-Jin Lu Krishna B S Swamy Jun-Yi Leu Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast. PLoS Genetics |
| title | Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast. |
| title_full | Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast. |
| title_fullStr | Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast. |
| title_full_unstemmed | Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast. |
| title_short | Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast. |
| title_sort | experimental evolution reveals interplay between sch9 and polyploid stability in yeast |
| url | http://europepmc.org/articles/PMC5094715?pdf=render |
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