Method for plasmid-based antibiotic-free fermentation

Method for plasmid-based antibiotic-free fermentation

Abstract Background Antibiotic-based plasmid selection and maintenance is a core tool in molecular biology; however, while convenient, this strategy has numerous drawbacks for biological manufacturing. Overuse of antibiotics and antibiotic resistance genes (ARG) contributes to the development of ant...

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Bibliographic Details
Main Authors: Katherine E. Brechun, Marion Förschle, Marlen Schmidt, Harald Kranz
Format: Article
Language:English
Published: BMC 2024-01-01
Series:Microbial Cell Factories
Subjects:
Escherichia coli
Antibiotic-free
Plasmid maintenance
Genome engineering
Complementation
Fermentation
Online Access:https://doi.org/10.1186/s12934-023-02291-z

Internet

https://doi.org/10.1186/s12934-023-02291-z

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