Evaluation of Toxicological Risks and Effects of Microplastics on Nile Tilapia (Oreochromis niloticus) under in Vitro Laboratory Conditions
Microplastics have been reported by many literatures as contaminants of environmental water bodies and are ingested by aquatic organisms due to their small sizes. Knowing the effects of microplastics to freshwater fish which are kept in ponds helps in managing fish-keeping practices. The objective o...
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| Format: | Article |
| Language: | English |
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Hawassa University
2023-10-01
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| Series: | East African Journal of Biophysical and Computational Sciences |
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| _version_ | 1797389545409347584 |
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| author | Macarius Pancras Mtega Matobola Joel Mihale Kessy Fidel Kilulya |
| author_facet | Macarius Pancras Mtega Matobola Joel Mihale Kessy Fidel Kilulya |
| author_sort | Macarius Pancras Mtega |
| collection | DOAJ |
| description | Microplastics have been reported by many literatures as contaminants of environmental water bodies and are ingested by aquatic organisms due to their small sizes. Knowing the effects of microplastics to freshwater fish which are kept in ponds helps in managing fish-keeping practices. The objective of the research was to determine the toxicity of microplastics to Nile tilapia (Oreochromis niloticus). The Experiment was done in 80 fish samples. Microplastics which were prepared for the batch experiment were introduced in the aquarium followed with observation for 21 days. The digestion of fish gills and intestines involved 10 mL of 10% (w/v) KOH solution and incubation at 65oC for 24 hours. Engulfed microplastics were determined using stereo microscope and At-IR spectrophotometer for confirmation. Engulfed microplastics were observed to be in mean range of 3.37 x 102±4.04 x 102 to 2.32 x 103 ± 3.57 x 103 particles/kg in gills and 4.68 x 104 ± 3.02 x 104 to4.40 x 104 ± 5.34 x 104 particles/kg in intestines. The observed responses were loss of equilibrium for 35% of fish, abnormal swimming for 49% of fish, abnormal ventilator behavior for 59% of fish, abnormal appearance for 39% of fish and average growth weight increase in control experiment fish was 6.10±2.62 g compared to 1.7 ± 3.62 g in test fish. There was no mortality of Nile tilapia. The responses of fish to the presence of microplastics in aquarium indicated that microplastics had adverse effects to Nile tilapia (Oreochromis niloticus). More researches have to be done on fish physiological changes caused by microplastics |
| first_indexed | 2024-03-08T22:57:28Z |
| format | Article |
| id | doaj.art-5e5738a706204212a874d2d8fe941289 |
| institution | Directory Open Access Journal |
| issn | 2789-360X 2789-3618 |
| language | English |
| last_indexed | 2024-03-08T22:57:28Z |
| publishDate | 2023-10-01 |
| publisher | Hawassa University |
| record_format | Article |
| series | East African Journal of Biophysical and Computational Sciences |
| spelling | doaj.art-5e5738a706204212a874d2d8fe9412892023-12-16T05:08:51ZengHawassa UniversityEast African Journal of Biophysical and Computational Sciences2789-360X2789-36182023-10-01425468https://dx.doi.org/10.4314/eajbcs.v4i2.5SEvaluation of Toxicological Risks and Effects of Microplastics on Nile Tilapia (Oreochromis niloticus) under in Vitro Laboratory ConditionsMacarius Pancras Mtega0Matobola Joel Mihale1Kessy Fidel Kilulya2Department of Physical and Environmental Sciences. Faculty of Science, Technology andEnvironmental Studies. Open University of TanzaniaDepartment of Physical and Environmental Sciences. Faculty of Science, Technology andEnvironmental Studies. Open University of TanzaniaDepartment of Chemistry, College of Natural and Applied Sciences, University of Dar es SalaamMicroplastics have been reported by many literatures as contaminants of environmental water bodies and are ingested by aquatic organisms due to their small sizes. Knowing the effects of microplastics to freshwater fish which are kept in ponds helps in managing fish-keeping practices. The objective of the research was to determine the toxicity of microplastics to Nile tilapia (Oreochromis niloticus). The Experiment was done in 80 fish samples. Microplastics which were prepared for the batch experiment were introduced in the aquarium followed with observation for 21 days. The digestion of fish gills and intestines involved 10 mL of 10% (w/v) KOH solution and incubation at 65oC for 24 hours. Engulfed microplastics were determined using stereo microscope and At-IR spectrophotometer for confirmation. Engulfed microplastics were observed to be in mean range of 3.37 x 102±4.04 x 102 to 2.32 x 103 ± 3.57 x 103 particles/kg in gills and 4.68 x 104 ± 3.02 x 104 to4.40 x 104 ± 5.34 x 104 particles/kg in intestines. The observed responses were loss of equilibrium for 35% of fish, abnormal swimming for 49% of fish, abnormal ventilator behavior for 59% of fish, abnormal appearance for 39% of fish and average growth weight increase in control experiment fish was 6.10±2.62 g compared to 1.7 ± 3.62 g in test fish. There was no mortality of Nile tilapia. The responses of fish to the presence of microplastics in aquarium indicated that microplastics had adverse effects to Nile tilapia (Oreochromis niloticus). More researches have to be done on fish physiological changes caused by microplasticsmicroplastics; toxicological risks; oreochromis niloticus; aquatic organisms; biological effects; in vitro condition |
| spellingShingle | Macarius Pancras Mtega Matobola Joel Mihale Kessy Fidel Kilulya Evaluation of Toxicological Risks and Effects of Microplastics on Nile Tilapia (Oreochromis niloticus) under in Vitro Laboratory Conditions East African Journal of Biophysical and Computational Sciences microplastics; toxicological risks; oreochromis niloticus; aquatic organisms; biological effects; in vitro condition |
| title | Evaluation of Toxicological Risks and Effects of Microplastics on Nile Tilapia (Oreochromis niloticus) under in Vitro Laboratory Conditions |
| title_full | Evaluation of Toxicological Risks and Effects of Microplastics on Nile Tilapia (Oreochromis niloticus) under in Vitro Laboratory Conditions |
| title_fullStr | Evaluation of Toxicological Risks and Effects of Microplastics on Nile Tilapia (Oreochromis niloticus) under in Vitro Laboratory Conditions |
| title_full_unstemmed | Evaluation of Toxicological Risks and Effects of Microplastics on Nile Tilapia (Oreochromis niloticus) under in Vitro Laboratory Conditions |
| title_short | Evaluation of Toxicological Risks and Effects of Microplastics on Nile Tilapia (Oreochromis niloticus) under in Vitro Laboratory Conditions |
| title_sort | evaluation of toxicological risks and effects of microplastics on nile tilapia oreochromis niloticus under in vitro laboratory conditions |
| topic | microplastics; toxicological risks; oreochromis niloticus; aquatic organisms; biological effects; in vitro condition |
| work_keys_str_mv | AT macariuspancrasmtega evaluationoftoxicologicalrisksandeffectsofmicroplasticsonniletilapiaoreochromisniloticusunderinvitrolaboratoryconditions AT matobolajoelmihale evaluationoftoxicologicalrisksandeffectsofmicroplasticsonniletilapiaoreochromisniloticusunderinvitrolaboratoryconditions AT kessyfidelkilulya evaluationoftoxicologicalrisksandeffectsofmicroplasticsonniletilapiaoreochromisniloticusunderinvitrolaboratoryconditions |