MOLECULAR CLONING, BIOINFORMATION ANALYSIS AND EXPRESSION OF THE STRICTOSIDINE SYNTHASE IN Dendrobium officinale
The enzyme strictosidine synthase (STR, EC: 4.3.3.2) plays a key role in the biosynthetic pathway of terpenoid indole alkaloid (TIA). It catalyzes the condensation of the tryptamine and secologanin to form 3α(S)-strictosidine, which is the common precursor of all TIAs. In this paper, a STR gene des...
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University of Life Sciences in Lublin - Publishing House
2020-06-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
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| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2124 |
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| author | Yan-Fang Zhu Hong-Hong Fan Da-Hui Li Qing Jin Chuan-Ming Zhang Li-Qin Zhu Cheng Song Yong-Ping Cai Yi Lin |
| author_facet | Yan-Fang Zhu Hong-Hong Fan Da-Hui Li Qing Jin Chuan-Ming Zhang Li-Qin Zhu Cheng Song Yong-Ping Cai Yi Lin |
| author_sort | Yan-Fang Zhu |
| collection | DOAJ |
| description |
The enzyme strictosidine synthase (STR, EC: 4.3.3.2) plays a key role in the biosynthetic pathway of terpenoid indole alkaloid (TIA). It catalyzes the condensation of the tryptamine and secologanin to form 3α(S)-strictosidine, which is the common precursor of all TIAs. In this paper, a STR gene designated as DoSTR (GenBank: KX068707) was first cloned and characterized from Dendrobium officinale with rapid amplified cDNA ends method (RACE). DoSTR has a length of 1380bp with 1179bp open reading frame encoding 392 amino acids. BlastP analyses showed that its amino acid sequence was classified into Str_synth superfamily. qRT-PCR showed that DoSTR was expressed in all tissues tested, with a significantly higher level in flower and the lowest in stem. Four different treatments with MeJA, SA, ABA and AgNO3, respectively, could induce the DoSTR expression to a different extent. And the effect of MeJA was the most obvious and transcript level of DoSTR induced by MeJA was 20.7 times greater than that of control at 48 hours after treatment. Furthermore, it was found that DoSTR was localized in vacuole through transient expression in tobacco. The characterization and expression of DoSTR can help in further studying the role of DoSTR in the biosynthesis of TIAs in D. officinale. This study may throw light on the alkaloid biosynthesis pathway of D. officinale.
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| id | doaj.art-5e5e3f4080164cf0b130218ddf28a0d2 |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:16:04Z |
| publishDate | 2020-06-01 |
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| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-5e5e3f4080164cf0b130218ddf28a0d22023-12-12T07:51:36ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052020-06-0119310.24326/asphc.2020.3.10MOLECULAR CLONING, BIOINFORMATION ANALYSIS AND EXPRESSION OF THE STRICTOSIDINE SYNTHASE IN Dendrobium officinaleYan-Fang Zhu0Hong-Hong Fan1Da-Hui Li2Qing Jin3Chuan-Ming Zhang4Li-Qin Zhu5Cheng Song6Yong-Ping Cai7Yi Lin8Key Laboratory of Resource Plant Biology of Anhui Province, School of Life Sciences, Huaibei Normal University, Huaibei 235000, P.R. China; School of Life Sciences, Anhui Agricultural University, Hefei, 230036, P.R. ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei, 230036, P.R. ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei, 230036, P.R. ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei, 230036, P.R. ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei, 230036, P.R. ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei, 230036, P.R. ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei, 230036, P.R. ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei, 230036, P.R. ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei, 230036, P.R. China The enzyme strictosidine synthase (STR, EC: 4.3.3.2) plays a key role in the biosynthetic pathway of terpenoid indole alkaloid (TIA). It catalyzes the condensation of the tryptamine and secologanin to form 3α(S)-strictosidine, which is the common precursor of all TIAs. In this paper, a STR gene designated as DoSTR (GenBank: KX068707) was first cloned and characterized from Dendrobium officinale with rapid amplified cDNA ends method (RACE). DoSTR has a length of 1380bp with 1179bp open reading frame encoding 392 amino acids. BlastP analyses showed that its amino acid sequence was classified into Str_synth superfamily. qRT-PCR showed that DoSTR was expressed in all tissues tested, with a significantly higher level in flower and the lowest in stem. Four different treatments with MeJA, SA, ABA and AgNO3, respectively, could induce the DoSTR expression to a different extent. And the effect of MeJA was the most obvious and transcript level of DoSTR induced by MeJA was 20.7 times greater than that of control at 48 hours after treatment. Furthermore, it was found that DoSTR was localized in vacuole through transient expression in tobacco. The characterization and expression of DoSTR can help in further studying the role of DoSTR in the biosynthesis of TIAs in D. officinale. This study may throw light on the alkaloid biosynthesis pathway of D. officinale. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2124Dendrobium officinaleDoSTRterpenoid indole alkaloidtissue expression patternsubcellular localization |
| spellingShingle | Yan-Fang Zhu Hong-Hong Fan Da-Hui Li Qing Jin Chuan-Ming Zhang Li-Qin Zhu Cheng Song Yong-Ping Cai Yi Lin MOLECULAR CLONING, BIOINFORMATION ANALYSIS AND EXPRESSION OF THE STRICTOSIDINE SYNTHASE IN Dendrobium officinale Acta Scientiarum Polonorum: Hortorum Cultus Dendrobium officinale DoSTR terpenoid indole alkaloid tissue expression pattern subcellular localization |
| title | MOLECULAR CLONING, BIOINFORMATION ANALYSIS AND EXPRESSION OF THE STRICTOSIDINE SYNTHASE IN Dendrobium officinale |
| title_full | MOLECULAR CLONING, BIOINFORMATION ANALYSIS AND EXPRESSION OF THE STRICTOSIDINE SYNTHASE IN Dendrobium officinale |
| title_fullStr | MOLECULAR CLONING, BIOINFORMATION ANALYSIS AND EXPRESSION OF THE STRICTOSIDINE SYNTHASE IN Dendrobium officinale |
| title_full_unstemmed | MOLECULAR CLONING, BIOINFORMATION ANALYSIS AND EXPRESSION OF THE STRICTOSIDINE SYNTHASE IN Dendrobium officinale |
| title_short | MOLECULAR CLONING, BIOINFORMATION ANALYSIS AND EXPRESSION OF THE STRICTOSIDINE SYNTHASE IN Dendrobium officinale |
| title_sort | molecular cloning bioinformation analysis and expression of the strictosidine synthase in dendrobium officinale |
| topic | Dendrobium officinale DoSTR terpenoid indole alkaloid tissue expression pattern subcellular localization |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2124 |
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