Comparative proteomic analysis of mustard lung as a complicated disease using systems biology approach
Abstract During Iraq-Iran conflict, chemical weapons, particularly SM gas, were used numerous times, whose aftereffects are still present. This study aimed to compare serum proteome in the chronic ML (n = 10) and HC (n = 10). TMT label-based quantitative proteomics was used to examine serums from tw...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Pulmonary Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12890-022-02240-3 |
| _version_ | 1828281133952925696 |
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| author | Shahram Parvin Masoud Arabfard Ali Ghazvini Mostafa Ghanei Ali Najafi |
| author_facet | Shahram Parvin Masoud Arabfard Ali Ghazvini Mostafa Ghanei Ali Najafi |
| author_sort | Shahram Parvin |
| collection | DOAJ |
| description | Abstract During Iraq-Iran conflict, chemical weapons, particularly SM gas, were used numerous times, whose aftereffects are still present. This study aimed to compare serum proteome in the chronic ML (n = 10) and HC (n = 10). TMT label-based quantitative proteomics was used to examine serums from two groups. Among total significant proteins, 14 proteins were upregulated (log2 ≥ FC 0.5, p 0.05), and 6 proteins were downregulated (log2 ≤ FC − 0.5, p 0.05). By helping PPI network, and EA, 11 main pathways connected to significantly different protein expression levels were discovered, including inflammatory and cell adhesion signaling pathways. It may be deduced that the wounded organs of exposed individuals experience poor repair cycles of cell degeneration and regeneration because certain repair signals were elevated while other structural and adhesion molecules were downregulated. The systems biology approach can help enhance our basic knowledge of biological processes, and contribute to a deeper understanding of pathophysiological mechanisms, as well as the identification of potential biomarkers of disease. |
| first_indexed | 2024-04-13T08:10:17Z |
| format | Article |
| id | doaj.art-5e5e6c37e904492c868132c2e056433a |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-04-13T08:10:17Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-5e5e6c37e904492c868132c2e056433a2022-12-22T02:55:00ZengBMCBMC Pulmonary Medicine1471-24662022-11-0122111010.1186/s12890-022-02240-3Comparative proteomic analysis of mustard lung as a complicated disease using systems biology approachShahram Parvin0Masoud Arabfard1Ali Ghazvini2Mostafa Ghanei3Ali Najafi4Education Office, Pasteur Institute of IranChemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical SciencesChemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical SciencesChemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical SciencesMolecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical SciencesAbstract During Iraq-Iran conflict, chemical weapons, particularly SM gas, were used numerous times, whose aftereffects are still present. This study aimed to compare serum proteome in the chronic ML (n = 10) and HC (n = 10). TMT label-based quantitative proteomics was used to examine serums from two groups. Among total significant proteins, 14 proteins were upregulated (log2 ≥ FC 0.5, p 0.05), and 6 proteins were downregulated (log2 ≤ FC − 0.5, p 0.05). By helping PPI network, and EA, 11 main pathways connected to significantly different protein expression levels were discovered, including inflammatory and cell adhesion signaling pathways. It may be deduced that the wounded organs of exposed individuals experience poor repair cycles of cell degeneration and regeneration because certain repair signals were elevated while other structural and adhesion molecules were downregulated. The systems biology approach can help enhance our basic knowledge of biological processes, and contribute to a deeper understanding of pathophysiological mechanisms, as well as the identification of potential biomarkers of disease.https://doi.org/10.1186/s12890-022-02240-3Mustard lungSystems biologyProteomicsEnrichment analysis |
| spellingShingle | Shahram Parvin Masoud Arabfard Ali Ghazvini Mostafa Ghanei Ali Najafi Comparative proteomic analysis of mustard lung as a complicated disease using systems biology approach BMC Pulmonary Medicine Mustard lung Systems biology Proteomics Enrichment analysis |
| title | Comparative proteomic analysis of mustard lung as a complicated disease using systems biology approach |
| title_full | Comparative proteomic analysis of mustard lung as a complicated disease using systems biology approach |
| title_fullStr | Comparative proteomic analysis of mustard lung as a complicated disease using systems biology approach |
| title_full_unstemmed | Comparative proteomic analysis of mustard lung as a complicated disease using systems biology approach |
| title_short | Comparative proteomic analysis of mustard lung as a complicated disease using systems biology approach |
| title_sort | comparative proteomic analysis of mustard lung as a complicated disease using systems biology approach |
| topic | Mustard lung Systems biology Proteomics Enrichment analysis |
| url | https://doi.org/10.1186/s12890-022-02240-3 |
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