Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications
Abstract The pathogenesis and complications of type 2 diabetes (T2DM) are closely linked with defective glucose metabolism, obesity, cardiovascular disease and an inability to mount an effective immune response to certain pathogenic organisms. Perturbations in key innate immune receptors known as To...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-07230-8 |
| _version_ | 1818749224642150400 |
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| author | Saket Gupta Ashwini Maratha Jakub Siednienko Anandan Natarajan Thusitha Gajanayake Shu Hoashi Sinéad Miggin |
| author_facet | Saket Gupta Ashwini Maratha Jakub Siednienko Anandan Natarajan Thusitha Gajanayake Shu Hoashi Sinéad Miggin |
| author_sort | Saket Gupta |
| collection | DOAJ |
| description | Abstract The pathogenesis and complications of type 2 diabetes (T2DM) are closely linked with defective glucose metabolism, obesity, cardiovascular disease and an inability to mount an effective immune response to certain pathogenic organisms. Perturbations in key innate immune receptors known as Toll-like receptors (TLRs) and inflammatory mediators such as IL-6, TNFα and IL-1β have been linked with T2DM. Herein, we sought to establish whether patients with T2DM and underlying complications exhibit perturbations in cytokine and TLR expression. Serum cytokine and mRNA levels of cytokines/TLRs in monocytes (M) and neutrophils (N) were measured in a cohort of 112 diabetic patients: good glycaemic control without complications (GC), good glycaemic control with complications (GCC), poor glycaemic control without complications (PC) and poor glycaemic control with complications (PCC) and compared them with 34 non-diabetic volunteers (NGT). Serum cytokine levels were normal in all study participants. In the GC group, cytokine and TLR gene expression were enhanced compared to NGT. In contrast, suppressed cytokine and TLR gene expression were evident in PC, GCC & PCC groups when compared to the GC. In conclusion, whereas serum pro-inflammatory cytokine levels are unaltered in T2DM patients, differences in inflammatory gene profiles exist among the T2DM patient groups. |
| first_indexed | 2024-12-18T04:00:23Z |
| format | Article |
| id | doaj.art-5e5f494fbf684e968ca4a373c602bbd9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:00:23Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5e5f494fbf684e968ca4a373c602bbd92022-12-21T21:21:42ZengNature PortfolioScientific Reports2045-23222017-08-017111010.1038/s41598-017-07230-8Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complicationsSaket Gupta0Ashwini Maratha1Jakub Siednienko2Anandan Natarajan3Thusitha Gajanayake4Shu Hoashi5Sinéad Miggin6Midlands Regional HospitalImmune Signalling laboratory, Institute of Immunology, Department of Biology, Maynooth UniversityImmune Signalling laboratory, Institute of Immunology, Department of Biology, Maynooth UniversityMidlands Regional HospitalImmune Signalling laboratory, Institute of Immunology, Department of Biology, Maynooth UniversityMidlands Regional HospitalImmune Signalling laboratory, Institute of Immunology, Department of Biology, Maynooth UniversityAbstract The pathogenesis and complications of type 2 diabetes (T2DM) are closely linked with defective glucose metabolism, obesity, cardiovascular disease and an inability to mount an effective immune response to certain pathogenic organisms. Perturbations in key innate immune receptors known as Toll-like receptors (TLRs) and inflammatory mediators such as IL-6, TNFα and IL-1β have been linked with T2DM. Herein, we sought to establish whether patients with T2DM and underlying complications exhibit perturbations in cytokine and TLR expression. Serum cytokine and mRNA levels of cytokines/TLRs in monocytes (M) and neutrophils (N) were measured in a cohort of 112 diabetic patients: good glycaemic control without complications (GC), good glycaemic control with complications (GCC), poor glycaemic control without complications (PC) and poor glycaemic control with complications (PCC) and compared them with 34 non-diabetic volunteers (NGT). Serum cytokine levels were normal in all study participants. In the GC group, cytokine and TLR gene expression were enhanced compared to NGT. In contrast, suppressed cytokine and TLR gene expression were evident in PC, GCC & PCC groups when compared to the GC. In conclusion, whereas serum pro-inflammatory cytokine levels are unaltered in T2DM patients, differences in inflammatory gene profiles exist among the T2DM patient groups.https://doi.org/10.1038/s41598-017-07230-8 |
| spellingShingle | Saket Gupta Ashwini Maratha Jakub Siednienko Anandan Natarajan Thusitha Gajanayake Shu Hoashi Sinéad Miggin Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications Scientific Reports |
| title | Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications |
| title_full | Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications |
| title_fullStr | Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications |
| title_full_unstemmed | Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications |
| title_short | Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications |
| title_sort | analysis of inflammatory cytokine and tlr expression levels in type 2 diabetes with complications |
| url | https://doi.org/10.1038/s41598-017-07230-8 |
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