Microbiome Composition and Microbial Community Structure in Mosquito Vectors <i>Aedes aegypti</i> and <i>Aedes albopictus</i> in Northeastern Thailand, a Dengue-Endemic Area
Bacterial content in mosquito larvae and adults is altered by dynamic interactions during life and varies substantially in variety and composition depending on mosquito biology and ecology. This study aimed to identify the microbiota in <i>Aedes aegypti</i> and <i>Aedes albopictus&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/2/184 |
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| author | Rutchanee Rodpai Patcharaporn Boonroumkaew Lakkhana Sadaow Oranuch Sanpool Penchom Janwan Tongjit Thanchomnang Pewpan M. Intapan Wanchai Maleewong |
| author_facet | Rutchanee Rodpai Patcharaporn Boonroumkaew Lakkhana Sadaow Oranuch Sanpool Penchom Janwan Tongjit Thanchomnang Pewpan M. Intapan Wanchai Maleewong |
| author_sort | Rutchanee Rodpai |
| collection | DOAJ |
| description | Bacterial content in mosquito larvae and adults is altered by dynamic interactions during life and varies substantially in variety and composition depending on mosquito biology and ecology. This study aimed to identify the microbiota in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> and in water from their breeding sites in northeastern Thailand, a dengue-endemic area. Bacterial diversity in field-collected aquatic larvae and subsequently emerged adults of both species from several locations were examined. The microbiota was characterized based on analysis of DNA sequences from the V3-V4 region of the 16S rRNA gene and exhibited changes during development, from the mosquito larval stage to the adult stage. <i>Aedes aegypti</i> contained a significantly higher number of bacterial genera than did <i>Ae. albopictus</i>, except for the genus <i>Wolbachia</i>, which was present at significantly higher frequencies in male <i>Ae. albopictus</i> (<i>p</i> < 0.05). Our findings also indicate likely transstadial transmission from larva to adult and give better understanding of the microbial diversity in these mosquitoes, informing future control programs against mosquito-borne diseases. |
| first_indexed | 2024-03-11T08:38:39Z |
| format | Article |
| id | doaj.art-5e63c03ee7a644b1bae11dab8db9e6b3 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T08:38:39Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-5e63c03ee7a644b1bae11dab8db9e6b32023-11-16T21:14:59ZengMDPI AGInsects2075-44502023-02-0114218410.3390/insects14020184Microbiome Composition and Microbial Community Structure in Mosquito Vectors <i>Aedes aegypti</i> and <i>Aedes albopictus</i> in Northeastern Thailand, a Dengue-Endemic AreaRutchanee Rodpai0Patcharaporn Boonroumkaew1Lakkhana Sadaow2Oranuch Sanpool3Penchom Janwan4Tongjit Thanchomnang5Pewpan M. Intapan6Wanchai Maleewong7Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80161, ThailandFaculty of Medicine, Mahasarakham University, Maha Sarakham 44000, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandBacterial content in mosquito larvae and adults is altered by dynamic interactions during life and varies substantially in variety and composition depending on mosquito biology and ecology. This study aimed to identify the microbiota in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> and in water from their breeding sites in northeastern Thailand, a dengue-endemic area. Bacterial diversity in field-collected aquatic larvae and subsequently emerged adults of both species from several locations were examined. The microbiota was characterized based on analysis of DNA sequences from the V3-V4 region of the 16S rRNA gene and exhibited changes during development, from the mosquito larval stage to the adult stage. <i>Aedes aegypti</i> contained a significantly higher number of bacterial genera than did <i>Ae. albopictus</i>, except for the genus <i>Wolbachia</i>, which was present at significantly higher frequencies in male <i>Ae. albopictus</i> (<i>p</i> < 0.05). Our findings also indicate likely transstadial transmission from larva to adult and give better understanding of the microbial diversity in these mosquitoes, informing future control programs against mosquito-borne diseases.https://www.mdpi.com/2075-4450/14/2/184microbiota<i>Aedes aegypti</i><i>Aedes albopictus</i>northeastern Thailanddengue vectormosquito vector |
| spellingShingle | Rutchanee Rodpai Patcharaporn Boonroumkaew Lakkhana Sadaow Oranuch Sanpool Penchom Janwan Tongjit Thanchomnang Pewpan M. Intapan Wanchai Maleewong Microbiome Composition and Microbial Community Structure in Mosquito Vectors <i>Aedes aegypti</i> and <i>Aedes albopictus</i> in Northeastern Thailand, a Dengue-Endemic Area Insects microbiota <i>Aedes aegypti</i> <i>Aedes albopictus</i> northeastern Thailand dengue vector mosquito vector |
| title | Microbiome Composition and Microbial Community Structure in Mosquito Vectors <i>Aedes aegypti</i> and <i>Aedes albopictus</i> in Northeastern Thailand, a Dengue-Endemic Area |
| title_full | Microbiome Composition and Microbial Community Structure in Mosquito Vectors <i>Aedes aegypti</i> and <i>Aedes albopictus</i> in Northeastern Thailand, a Dengue-Endemic Area |
| title_fullStr | Microbiome Composition and Microbial Community Structure in Mosquito Vectors <i>Aedes aegypti</i> and <i>Aedes albopictus</i> in Northeastern Thailand, a Dengue-Endemic Area |
| title_full_unstemmed | Microbiome Composition and Microbial Community Structure in Mosquito Vectors <i>Aedes aegypti</i> and <i>Aedes albopictus</i> in Northeastern Thailand, a Dengue-Endemic Area |
| title_short | Microbiome Composition and Microbial Community Structure in Mosquito Vectors <i>Aedes aegypti</i> and <i>Aedes albopictus</i> in Northeastern Thailand, a Dengue-Endemic Area |
| title_sort | microbiome composition and microbial community structure in mosquito vectors i aedes aegypti i and i aedes albopictus i in northeastern thailand a dengue endemic area |
| topic | microbiota <i>Aedes aegypti</i> <i>Aedes albopictus</i> northeastern Thailand dengue vector mosquito vector |
| url | https://www.mdpi.com/2075-4450/14/2/184 |
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