Analgesic effects of Marasmius androsaceus mycelia ethanol extract and possible mechanisms in mice
Marasmius androsaceus is a medicinal fungus mainly used to treat various forms of pain in China. This study investigated the analgesic effects of an ethanol extract of M. androsaceus (MAE) and its potential molecular mechanisms. Oral administration of MAE (50, 200, and 1000 mg/kg) had significant an...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Associação Brasileira de Divulgação Científica
2018-03-01
|
| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400613&lng=en&tlng=en |
| _version_ | 1828271991740694528 |
|---|---|
| author | Jia Song Xue Wang Yu Huang Yidi Qu Guirong Zhang Di Wang |
| author_facet | Jia Song Xue Wang Yu Huang Yidi Qu Guirong Zhang Di Wang |
| author_sort | Jia Song |
| collection | DOAJ |
| description | Marasmius androsaceus is a medicinal fungus mainly used to treat various forms of pain in China. This study investigated the analgesic effects of an ethanol extract of M. androsaceus (MAE) and its potential molecular mechanisms. Oral administration of MAE (50, 200, and 1000 mg/kg) had significant analgesic effects in an acid-induced writhing test, a formalin test, and a hot-plate test, with effectiveness similar to tramadol (the positive control drug). The autonomic activity test showed that MAE had no harmful effects on the central nervous system in mice. MAE resulted in significantly enhanced levels of noradrenalin and 5-hydroxytryptamine in serum but suppressed both of these neurotransmitters in the hypothalamus after 30 s of hot-plate stimulation. Co-administration with nimodipine (10 mg/kg; a Ca2+ channel blocker) strongly enhanced the analgesic effect in the hot-plate test compared to MAE alone. Moreover, MAE down-regulated the expression of calmodulin-dependent protein kinase II (CaMKII) in the hypothalamus after a 30-s thermal stimulus. These results suggested that the analgesic ability of MAE is related to the regulation of metabolism by monoamine neurotransmitters and Ca2+/CaMKII-mediated signaling, which can potentially aid the development of peripheral neuropathic pain treatments obtained from M. androsaceus. |
| first_indexed | 2024-04-13T06:00:28Z |
| format | Article |
| id | doaj.art-5e65a76f03dd48e0ac6b7d72d88080a8 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-13T06:00:28Z |
| publishDate | 2018-03-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-5e65a76f03dd48e0ac6b7d72d88080a82022-12-22T02:59:28ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2018-03-0151410.1590/1414-431x20177124S0100-879X2018000400613Analgesic effects of Marasmius androsaceus mycelia ethanol extract and possible mechanisms in miceJia SongXue WangYu HuangYidi QuGuirong ZhangDi WangMarasmius androsaceus is a medicinal fungus mainly used to treat various forms of pain in China. This study investigated the analgesic effects of an ethanol extract of M. androsaceus (MAE) and its potential molecular mechanisms. Oral administration of MAE (50, 200, and 1000 mg/kg) had significant analgesic effects in an acid-induced writhing test, a formalin test, and a hot-plate test, with effectiveness similar to tramadol (the positive control drug). The autonomic activity test showed that MAE had no harmful effects on the central nervous system in mice. MAE resulted in significantly enhanced levels of noradrenalin and 5-hydroxytryptamine in serum but suppressed both of these neurotransmitters in the hypothalamus after 30 s of hot-plate stimulation. Co-administration with nimodipine (10 mg/kg; a Ca2+ channel blocker) strongly enhanced the analgesic effect in the hot-plate test compared to MAE alone. Moreover, MAE down-regulated the expression of calmodulin-dependent protein kinase II (CaMKII) in the hypothalamus after a 30-s thermal stimulus. These results suggested that the analgesic ability of MAE is related to the regulation of metabolism by monoamine neurotransmitters and Ca2+/CaMKII-mediated signaling, which can potentially aid the development of peripheral neuropathic pain treatments obtained from M. androsaceus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400613&lng=en&tlng=enMarasmius androsaceusAnalgesic abilityMonoamine neurotransmittersCalcium channels |
| spellingShingle | Jia Song Xue Wang Yu Huang Yidi Qu Guirong Zhang Di Wang Analgesic effects of Marasmius androsaceus mycelia ethanol extract and possible mechanisms in mice Brazilian Journal of Medical and Biological Research Marasmius androsaceus Analgesic ability Monoamine neurotransmitters Calcium channels |
| title | Analgesic effects of Marasmius androsaceus mycelia ethanol extract and possible mechanisms in mice |
| title_full | Analgesic effects of Marasmius androsaceus mycelia ethanol extract and possible mechanisms in mice |
| title_fullStr | Analgesic effects of Marasmius androsaceus mycelia ethanol extract and possible mechanisms in mice |
| title_full_unstemmed | Analgesic effects of Marasmius androsaceus mycelia ethanol extract and possible mechanisms in mice |
| title_short | Analgesic effects of Marasmius androsaceus mycelia ethanol extract and possible mechanisms in mice |
| title_sort | analgesic effects of marasmius androsaceus mycelia ethanol extract and possible mechanisms in mice |
| topic | Marasmius androsaceus Analgesic ability Monoamine neurotransmitters Calcium channels |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400613&lng=en&tlng=en |
| work_keys_str_mv | AT jiasong analgesiceffectsofmarasmiusandrosaceusmyceliaethanolextractandpossiblemechanismsinmice AT xuewang analgesiceffectsofmarasmiusandrosaceusmyceliaethanolextractandpossiblemechanismsinmice AT yuhuang analgesiceffectsofmarasmiusandrosaceusmyceliaethanolextractandpossiblemechanismsinmice AT yidiqu analgesiceffectsofmarasmiusandrosaceusmyceliaethanolextractandpossiblemechanismsinmice AT guirongzhang analgesiceffectsofmarasmiusandrosaceusmyceliaethanolextractandpossiblemechanismsinmice AT diwang analgesiceffectsofmarasmiusandrosaceusmyceliaethanolextractandpossiblemechanismsinmice |