Restorative effects of Rg3-enriched Korean Red Ginseng and Persicaria tinctoria extract on oxazolone-induced ulcerative colitis in mice
Background: Ulcerative colitis (UC) is the large intestine disease that results in chronic inflammation and ulcers in the colon. Rg3-enriched Korean Red Ginseng extract (Rg3-RGE) is known for its pharmacological activities. Persicaria tinctoria (PT) is also used in the treatment of various inflammat...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845321001056 |
| _version_ | 1811188802033549312 |
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| author | H.M. Arif Ullah Evelyn Saba Yuan Yee Lee Seung-Bok Hong Sun-Hee Hyun Yi-Seong Kwak Chae-Kyu Park Sung Dae Kim Man Hee Rhee |
| author_facet | H.M. Arif Ullah Evelyn Saba Yuan Yee Lee Seung-Bok Hong Sun-Hee Hyun Yi-Seong Kwak Chae-Kyu Park Sung Dae Kim Man Hee Rhee |
| author_sort | H.M. Arif Ullah |
| collection | DOAJ |
| description | Background: Ulcerative colitis (UC) is the large intestine disease that results in chronic inflammation and ulcers in the colon. Rg3-enriched Korean Red Ginseng extract (Rg3-RGE) is known for its pharmacological activities. Persicaria tinctoria (PT) is also used in the treatment of various inflammatory diseases. The aim of this study is to investigate the attenuating effects of Rg3-RGE with PT on oxazolone (OXA)-induced UC in mice. Methods: A total of six groups of mice including control group, OXA (as model group, 1.5%) group, sulfasalazine (75 mg/kg) group, Rg3-RGE (20 mg/kg) group, PT (300 mg/kg) group, and Rg3-RGE (10 mg/kg) with PT (150 mg/kg) group. Data on the colon length, body weight, disease activity index (DAI), histological changes, nitric oxide (NO) assay, Real-time PCR of inflammatory factors, ELISA of inflammatory factors, Western blot, and flow cytometry analysis were obtained. Results: Overall, the combination treatment of Rg3-RGE and PT significantly improved the colon length and body weight and decreased the DAI in mice compared with the treatment with OXA. Additionally, the histological injury was also reduced by the combination treatment. Moreover, the NO production level and inflammatory mediators and cytokines were significantly downregulated in the Rg3-RGE with the PT group compared with the model group. Also, NLR family pyrin domain containing 3 (NLRP3) inflammasome and nuclear factor kappa B (NF-κB) were suppressed in the combination treatment group compared with the OXA group. Furthermore, the number of immune cell subtypes of CD4+ T-helper cells, CD19+ B-cells, and CD4+ and CD25+ regulatory T-cells (Tregs) was improved in the Rg3-RGE with the PT group compared with the OXA group. Conclusion: Overall, the mixture of Rg3-RGE and PT is an effective therapeutic treatment for UC. |
| first_indexed | 2024-04-11T14:24:23Z |
| format | Article |
| id | doaj.art-5e67c2d45d934080bbc93aa3c6e21f62 |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-04-11T14:24:23Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-5e67c2d45d934080bbc93aa3c6e21f622022-12-22T04:18:55ZengElsevierJournal of Ginseng Research1226-84532022-09-01465628635Restorative effects of Rg3-enriched Korean Red Ginseng and Persicaria tinctoria extract on oxazolone-induced ulcerative colitis in miceH.M. Arif Ullah0Evelyn Saba1Yuan Yee Lee2Seung-Bok Hong3Sun-Hee Hyun4Yi-Seong Kwak5Chae-Kyu Park6Sung Dae Kim7Man Hee Rhee8Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of KoreaDepartment of Veterinary Biomedical Sciences, Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah-Arid Agriculture University, PakistanDepartment of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of KoreaDepartment of Clinical Laboratory Science, Chungbuk Health & Science University, Chungbuk, Republic of KoreaR&D Headquarters, Korean Ginseng cooperation, Daejeon, Republic of KoreaR&D Headquarters, Korean Ginseng cooperation, Daejeon, Republic of KoreaR&D Headquarters, Korean Ginseng cooperation, Daejeon, Republic of KoreaDepartment of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of KoreaDepartment of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea; Corresponding author. Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea.Background: Ulcerative colitis (UC) is the large intestine disease that results in chronic inflammation and ulcers in the colon. Rg3-enriched Korean Red Ginseng extract (Rg3-RGE) is known for its pharmacological activities. Persicaria tinctoria (PT) is also used in the treatment of various inflammatory diseases. The aim of this study is to investigate the attenuating effects of Rg3-RGE with PT on oxazolone (OXA)-induced UC in mice. Methods: A total of six groups of mice including control group, OXA (as model group, 1.5%) group, sulfasalazine (75 mg/kg) group, Rg3-RGE (20 mg/kg) group, PT (300 mg/kg) group, and Rg3-RGE (10 mg/kg) with PT (150 mg/kg) group. Data on the colon length, body weight, disease activity index (DAI), histological changes, nitric oxide (NO) assay, Real-time PCR of inflammatory factors, ELISA of inflammatory factors, Western blot, and flow cytometry analysis were obtained. Results: Overall, the combination treatment of Rg3-RGE and PT significantly improved the colon length and body weight and decreased the DAI in mice compared with the treatment with OXA. Additionally, the histological injury was also reduced by the combination treatment. Moreover, the NO production level and inflammatory mediators and cytokines were significantly downregulated in the Rg3-RGE with the PT group compared with the model group. Also, NLR family pyrin domain containing 3 (NLRP3) inflammasome and nuclear factor kappa B (NF-κB) were suppressed in the combination treatment group compared with the OXA group. Furthermore, the number of immune cell subtypes of CD4+ T-helper cells, CD19+ B-cells, and CD4+ and CD25+ regulatory T-cells (Tregs) was improved in the Rg3-RGE with the PT group compared with the OXA group. Conclusion: Overall, the mixture of Rg3-RGE and PT is an effective therapeutic treatment for UC.http://www.sciencedirect.com/science/article/pii/S1226845321001056Korean Red GinsengGinsenoside Rg3Persicaria tinctoriaInflammationColitis |
| spellingShingle | H.M. Arif Ullah Evelyn Saba Yuan Yee Lee Seung-Bok Hong Sun-Hee Hyun Yi-Seong Kwak Chae-Kyu Park Sung Dae Kim Man Hee Rhee Restorative effects of Rg3-enriched Korean Red Ginseng and Persicaria tinctoria extract on oxazolone-induced ulcerative colitis in mice Journal of Ginseng Research Korean Red Ginseng Ginsenoside Rg3 Persicaria tinctoria Inflammation Colitis |
| title | Restorative effects of Rg3-enriched Korean Red Ginseng and Persicaria tinctoria extract on oxazolone-induced ulcerative colitis in mice |
| title_full | Restorative effects of Rg3-enriched Korean Red Ginseng and Persicaria tinctoria extract on oxazolone-induced ulcerative colitis in mice |
| title_fullStr | Restorative effects of Rg3-enriched Korean Red Ginseng and Persicaria tinctoria extract on oxazolone-induced ulcerative colitis in mice |
| title_full_unstemmed | Restorative effects of Rg3-enriched Korean Red Ginseng and Persicaria tinctoria extract on oxazolone-induced ulcerative colitis in mice |
| title_short | Restorative effects of Rg3-enriched Korean Red Ginseng and Persicaria tinctoria extract on oxazolone-induced ulcerative colitis in mice |
| title_sort | restorative effects of rg3 enriched korean red ginseng and persicaria tinctoria extract on oxazolone induced ulcerative colitis in mice |
| topic | Korean Red Ginseng Ginsenoside Rg3 Persicaria tinctoria Inflammation Colitis |
| url | http://www.sciencedirect.com/science/article/pii/S1226845321001056 |
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