Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces
Coupling of cells to biomaterials is a prerequisite for most biomedical applications; e.g., neuroelectrodes can only stimulate brain tissue in vivo if the electric signal is transferred to neurons attached to the electrodes’ surface. Besides, cell survival in vitro also depends on the interaction of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/8588 |
| _version_ | 1797523634391089152 |
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| author | Alice Abend Chelsie Steele Heinz-Georg Jahnke Mareike Zink |
| author_facet | Alice Abend Chelsie Steele Heinz-Georg Jahnke Mareike Zink |
| author_sort | Alice Abend |
| collection | DOAJ |
| description | Coupling of cells to biomaterials is a prerequisite for most biomedical applications; e.g., neuroelectrodes can only stimulate brain tissue in vivo if the electric signal is transferred to neurons attached to the electrodes’ surface. Besides, cell survival in vitro also depends on the interaction of cells with the underlying substrate materials; in vitro assays such as multielectrode arrays determine cellular behavior by electrical coupling to the adherent cells. In our study, we investigated the interaction of neurons and glial cells with different electrode materials such as TiN and nanocolumnar TiN surfaces in contrast to gold and ITO substrates. Employing single-cell force spectroscopy, we quantified short-term interaction forces between neuron-like cells (SH-SY5Y cells) and glial cells (U-87 MG cells) for the different materials and contact times. Additionally, results were compared to the spreading dynamics of cells for different culture times as a function of the underlying substrate. The adhesion behavior of glial cells was almost independent of the biomaterial and the maximum growth areas were already seen after one day; however, adhesion dynamics of neurons relied on culture material and time. Neurons spread much better on TiN and nanocolumnar TiN and also formed more neurites after three days in culture. Our designed nanocolumnar TiN offers the possibility for building miniaturized microelectrode arrays for impedance spectroscopy without losing detection sensitivity due to a lowered self-impedance of the electrode. Hence, our results show that this biomaterial promotes adhesion and spreading of neurons and glial cells, which are important for many biomedical applications in vitro and in vivo. |
| first_indexed | 2024-03-10T08:44:44Z |
| format | Article |
| id | doaj.art-5e6afb7947fe44a09be994a700f320b5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:44Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5e6afb7947fe44a09be994a700f320b52023-11-22T07:57:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216858810.3390/ijms22168588Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine InterfacesAlice Abend0Chelsie Steele1Heinz-Georg Jahnke2Mareike Zink3Research Group Biotechnology and Biomedicine, Faculty of Physics and Earth Sciences, Peter Debye Institute for Soft Matter Physics, Leipzig University, Linnéstraße 5, 04103 Leipzig, GermanyResearch Group Biotechnology and Biomedicine, Faculty of Physics and Earth Sciences, Peter Debye Institute for Soft Matter Physics, Leipzig University, Linnéstraße 5, 04103 Leipzig, GermanyCentre for Biotechnology and Biomedicine, Molecular Biological-Biochemical Processing Technology, Leipzig University, Deutscher Platz 5, 04103 Leipzig, GermanyResearch Group Biotechnology and Biomedicine, Faculty of Physics and Earth Sciences, Peter Debye Institute for Soft Matter Physics, Leipzig University, Linnéstraße 5, 04103 Leipzig, GermanyCoupling of cells to biomaterials is a prerequisite for most biomedical applications; e.g., neuroelectrodes can only stimulate brain tissue in vivo if the electric signal is transferred to neurons attached to the electrodes’ surface. Besides, cell survival in vitro also depends on the interaction of cells with the underlying substrate materials; in vitro assays such as multielectrode arrays determine cellular behavior by electrical coupling to the adherent cells. In our study, we investigated the interaction of neurons and glial cells with different electrode materials such as TiN and nanocolumnar TiN surfaces in contrast to gold and ITO substrates. Employing single-cell force spectroscopy, we quantified short-term interaction forces between neuron-like cells (SH-SY5Y cells) and glial cells (U-87 MG cells) for the different materials and contact times. Additionally, results were compared to the spreading dynamics of cells for different culture times as a function of the underlying substrate. The adhesion behavior of glial cells was almost independent of the biomaterial and the maximum growth areas were already seen after one day; however, adhesion dynamics of neurons relied on culture material and time. Neurons spread much better on TiN and nanocolumnar TiN and also formed more neurites after three days in culture. Our designed nanocolumnar TiN offers the possibility for building miniaturized microelectrode arrays for impedance spectroscopy without losing detection sensitivity due to a lowered self-impedance of the electrode. Hence, our results show that this biomaterial promotes adhesion and spreading of neurons and glial cells, which are important for many biomedical applications in vitro and in vivo.https://www.mdpi.com/1422-0067/22/16/8588neuronsglial cellselectrode materialscell adhesioncell spreadingTiN |
| spellingShingle | Alice Abend Chelsie Steele Heinz-Georg Jahnke Mareike Zink Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces International Journal of Molecular Sciences neurons glial cells electrode materials cell adhesion cell spreading TiN |
| title | Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces |
| title_full | Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces |
| title_fullStr | Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces |
| title_full_unstemmed | Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces |
| title_short | Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces |
| title_sort | adhesion of neurons and glial cells with nanocolumnar tin films for brain machine interfaces |
| topic | neurons glial cells electrode materials cell adhesion cell spreading TiN |
| url | https://www.mdpi.com/1422-0067/22/16/8588 |
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