Transcriptome Profile Reveals Differences between Remote and Ischemic Myocardium after Acute Myocardial Infarction in a Swine Model
Acute myocardial infarction (AMI) is the consequence of an acute interruption of myocardial blood flow delimiting an area with ischemic necrosis. The loss of cardiomyocytes initiates cardiac remodeling in the myocardium, leading to molecular changes in an attempt to recover myocardial function. The...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/3/340 |
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| author | María Pulido María Ángeles de Pedro Verónica Álvarez Ana María Marchena Virginia Blanco-Blázquez Claudia Báez-Díaz Verónica Crisóstomo Javier G. Casado Francisco Miguel Sánchez-Margallo Esther López |
| author_facet | María Pulido María Ángeles de Pedro Verónica Álvarez Ana María Marchena Virginia Blanco-Blázquez Claudia Báez-Díaz Verónica Crisóstomo Javier G. Casado Francisco Miguel Sánchez-Margallo Esther López |
| author_sort | María Pulido |
| collection | DOAJ |
| description | Acute myocardial infarction (AMI) is the consequence of an acute interruption of myocardial blood flow delimiting an area with ischemic necrosis. The loss of cardiomyocytes initiates cardiac remodeling in the myocardium, leading to molecular changes in an attempt to recover myocardial function. The purpose of this study was to unravel the differences in the molecular profile between ischemic and remote myocardium after AMI in an experimental model. To mimic human myocardial infarction, healthy pigs were subjected to occlusion of the mid-left anterior descending coronary artery, and myocardial tissue was collected from ischemic and remote zones for omics techniques. Comparative transcriptome analysis of both areas was accurately validated by proteomic analysis, resulting in mitochondrion-related biological processes being the most impaired mechanisms in the infarcted area. Moreover, Immune system process-related genes were up-regulated in the remote tissue, mainly due to the increase of neutrophil migration in this area. These results provide valuable information regarding differentially expressed genes and their biological functions between ischemic and remote myocardium after AMI, which could be useful for establishing therapeutic targets for the development of new treatments. |
| first_indexed | 2024-03-11T06:54:29Z |
| format | Article |
| id | doaj.art-5e6c604bc0384f78a4c2d1de7ff77c00 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T06:54:29Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-5e6c604bc0384f78a4c2d1de7ff77c002023-11-17T09:40:46ZengMDPI AGBiology2079-77372023-02-0112334010.3390/biology12030340Transcriptome Profile Reveals Differences between Remote and Ischemic Myocardium after Acute Myocardial Infarction in a Swine ModelMaría Pulido0María Ángeles de Pedro1Verónica Álvarez2Ana María Marchena3Virginia Blanco-Blázquez4Claudia Báez-Díaz5Verónica Crisóstomo6Javier G. Casado7Francisco Miguel Sánchez-Margallo8Esther López9Jesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, SpainJesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, SpainJesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, SpainJesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, SpainJesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, SpainJesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, SpainJesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, SpainRICORS-TERAV Network, ISCIII, 28029 Madrid, SpainJesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, SpainJesús Usón Minimally Invasive Surgery Centre, Carretera Nacional 521, Km 41.8, 10071 Cáceres, SpainAcute myocardial infarction (AMI) is the consequence of an acute interruption of myocardial blood flow delimiting an area with ischemic necrosis. The loss of cardiomyocytes initiates cardiac remodeling in the myocardium, leading to molecular changes in an attempt to recover myocardial function. The purpose of this study was to unravel the differences in the molecular profile between ischemic and remote myocardium after AMI in an experimental model. To mimic human myocardial infarction, healthy pigs were subjected to occlusion of the mid-left anterior descending coronary artery, and myocardial tissue was collected from ischemic and remote zones for omics techniques. Comparative transcriptome analysis of both areas was accurately validated by proteomic analysis, resulting in mitochondrion-related biological processes being the most impaired mechanisms in the infarcted area. Moreover, Immune system process-related genes were up-regulated in the remote tissue, mainly due to the increase of neutrophil migration in this area. These results provide valuable information regarding differentially expressed genes and their biological functions between ischemic and remote myocardium after AMI, which could be useful for establishing therapeutic targets for the development of new treatments.https://www.mdpi.com/2079-7737/12/3/340acute myocardial infarctionischemic arearemote areaswine animal modeltranscriptome profiling |
| spellingShingle | María Pulido María Ángeles de Pedro Verónica Álvarez Ana María Marchena Virginia Blanco-Blázquez Claudia Báez-Díaz Verónica Crisóstomo Javier G. Casado Francisco Miguel Sánchez-Margallo Esther López Transcriptome Profile Reveals Differences between Remote and Ischemic Myocardium after Acute Myocardial Infarction in a Swine Model Biology acute myocardial infarction ischemic area remote area swine animal model transcriptome profiling |
| title | Transcriptome Profile Reveals Differences between Remote and Ischemic Myocardium after Acute Myocardial Infarction in a Swine Model |
| title_full | Transcriptome Profile Reveals Differences between Remote and Ischemic Myocardium after Acute Myocardial Infarction in a Swine Model |
| title_fullStr | Transcriptome Profile Reveals Differences between Remote and Ischemic Myocardium after Acute Myocardial Infarction in a Swine Model |
| title_full_unstemmed | Transcriptome Profile Reveals Differences between Remote and Ischemic Myocardium after Acute Myocardial Infarction in a Swine Model |
| title_short | Transcriptome Profile Reveals Differences between Remote and Ischemic Myocardium after Acute Myocardial Infarction in a Swine Model |
| title_sort | transcriptome profile reveals differences between remote and ischemic myocardium after acute myocardial infarction in a swine model |
| topic | acute myocardial infarction ischemic area remote area swine animal model transcriptome profiling |
| url | https://www.mdpi.com/2079-7737/12/3/340 |
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