| Summary: | A dinuclear Pt(II) complex with putrescine as bridging polyamine ligand ([Pt<sub>2</sub>Put<sub>2</sub>(NH<sub>3</sub>)<sub>4</sub>]Cl<sub>4</sub>) was synthesized and assessed as to its potential anticancer activity against a human non-small cell lung cancer line (A549), as well as towards non-cancer cells (BEAS-2B). This effect was evaluated through in vitro cytotoxicity assays (MTT and SRB) coupled to microFTIR and microRaman spectroscopies, the former delivering information on growth-inhibiting and cytotoxic abilities while the latter provided very specific information on the metabolic impact of the metal agent (at the sub-cellular level). Regarding cancer cells, a major impact of [Pt<sub>2</sub>Put<sub>2</sub>(NH<sub>3</sub>)<sub>4</sub>]Cl<sub>4</sub> was evidenced on cellular proteins and lipids, as compared to DNA, particularly via the Amide I and Amide II signals. The effect of the chelate on non-malignant cells was lower than on malignant ones, evidencing a promising low toxicity towards healthy cells.
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