Metabolites Analysis of Anti-Myocardial Ischemia Active Components of <i>Saussurea involucrata</i> Based on Gut Microbiota—Drug Interaction
<i>Saussurea involucrata</i> has been reported to have potential therapeutic effects against myocardial ischemia. The pharmacological effects of oral natural medicines may be influenced by the participation of gut microbiota. In this study, we aimed to investigate the bidirectional regul...
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/13/7457 |
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| author | Hang Yu Jie Fu Hui-Hui Guo Li-Bin Pan Hui Xu Zheng-Wei Zhang Jia-Chun Hu Xin-Yu Yang Hao-Jian Zhang Meng-Meng Bu Yuan Lin Jian-Dong Jiang Yan Wang |
| author_facet | Hang Yu Jie Fu Hui-Hui Guo Li-Bin Pan Hui Xu Zheng-Wei Zhang Jia-Chun Hu Xin-Yu Yang Hao-Jian Zhang Meng-Meng Bu Yuan Lin Jian-Dong Jiang Yan Wang |
| author_sort | Hang Yu |
| collection | DOAJ |
| description | <i>Saussurea involucrata</i> has been reported to have potential therapeutic effects against myocardial ischemia. The pharmacological effects of oral natural medicines may be influenced by the participation of gut microbiota. In this study, we aimed to investigate the bidirectional regulation of gut microbiota and the main components of <i>Saussurea involucrata</i>. We first established a quantitative method for the four main components (chlorogenic acid, syringin, acanthoside B, rutin) which were chosen by fingerprint using liquid chromatography tandem mass spectrometry (LC-MS/MS), and found that gut microbiota has a strong metabolic effect on them. Meanwhile, we identified five major rat gut microbiota metabolites (M1–M5) using liquid chromatography tandem time-of-flight mass spectrometry (LC/MS<sup>n</sup>-IT-TOF). The metabolic properties of metabolites in vitro were preliminarily elucidated by LC-MS/MS for the first time. These five metabolites of <i>Saussurea involucrata</i> may all have potential contributions to the treatment of myocardial ischemia. Furthermore, the four main components (10 μg/mL) can significantly stimulate intestinal bacteria to produce short chain fatty acids in vitro, respectively, which can further contribute to the effect in myocardial ischemia. In this study, the therapeutic effect against myocardial ischemia of <i>Saussurea involucrata</i> was first reported to be related to the intestinal flora, which can be useful in understanding the effective substances of <i>Saussurea involucrata</i>. |
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| last_indexed | 2024-03-09T21:46:44Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5e6f9a1072ba466e94f67e3205168e962023-11-23T20:14:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012313745710.3390/ijms23137457Metabolites Analysis of Anti-Myocardial Ischemia Active Components of <i>Saussurea involucrata</i> Based on Gut Microbiota—Drug InteractionHang Yu0Jie Fu1Hui-Hui Guo2Li-Bin Pan3Hui Xu4Zheng-Wei Zhang5Jia-Chun Hu6Xin-Yu Yang7Hao-Jian Zhang8Meng-Meng Bu9Yuan Lin10Jian-Dong Jiang11Yan Wang12State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, ChinaState Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, China<i>Saussurea involucrata</i> has been reported to have potential therapeutic effects against myocardial ischemia. The pharmacological effects of oral natural medicines may be influenced by the participation of gut microbiota. In this study, we aimed to investigate the bidirectional regulation of gut microbiota and the main components of <i>Saussurea involucrata</i>. We first established a quantitative method for the four main components (chlorogenic acid, syringin, acanthoside B, rutin) which were chosen by fingerprint using liquid chromatography tandem mass spectrometry (LC-MS/MS), and found that gut microbiota has a strong metabolic effect on them. Meanwhile, we identified five major rat gut microbiota metabolites (M1–M5) using liquid chromatography tandem time-of-flight mass spectrometry (LC/MS<sup>n</sup>-IT-TOF). The metabolic properties of metabolites in vitro were preliminarily elucidated by LC-MS/MS for the first time. These five metabolites of <i>Saussurea involucrata</i> may all have potential contributions to the treatment of myocardial ischemia. Furthermore, the four main components (10 μg/mL) can significantly stimulate intestinal bacteria to produce short chain fatty acids in vitro, respectively, which can further contribute to the effect in myocardial ischemia. In this study, the therapeutic effect against myocardial ischemia of <i>Saussurea involucrata</i> was first reported to be related to the intestinal flora, which can be useful in understanding the effective substances of <i>Saussurea involucrata</i>.https://www.mdpi.com/1422-0067/23/13/7457<i>Saussurea involucrata</i>gut microbiota<i>Tian-Shan-Xue-Lian</i>LC-MS/MSLC/MS<sup>n</sup>-IT-TOFmetabolite |
| spellingShingle | Hang Yu Jie Fu Hui-Hui Guo Li-Bin Pan Hui Xu Zheng-Wei Zhang Jia-Chun Hu Xin-Yu Yang Hao-Jian Zhang Meng-Meng Bu Yuan Lin Jian-Dong Jiang Yan Wang Metabolites Analysis of Anti-Myocardial Ischemia Active Components of <i>Saussurea involucrata</i> Based on Gut Microbiota—Drug Interaction International Journal of Molecular Sciences <i>Saussurea involucrata</i> gut microbiota <i>Tian-Shan-Xue-Lian</i> LC-MS/MS LC/MS<sup>n</sup>-IT-TOF metabolite |
| title | Metabolites Analysis of Anti-Myocardial Ischemia Active Components of <i>Saussurea involucrata</i> Based on Gut Microbiota—Drug Interaction |
| title_full | Metabolites Analysis of Anti-Myocardial Ischemia Active Components of <i>Saussurea involucrata</i> Based on Gut Microbiota—Drug Interaction |
| title_fullStr | Metabolites Analysis of Anti-Myocardial Ischemia Active Components of <i>Saussurea involucrata</i> Based on Gut Microbiota—Drug Interaction |
| title_full_unstemmed | Metabolites Analysis of Anti-Myocardial Ischemia Active Components of <i>Saussurea involucrata</i> Based on Gut Microbiota—Drug Interaction |
| title_short | Metabolites Analysis of Anti-Myocardial Ischemia Active Components of <i>Saussurea involucrata</i> Based on Gut Microbiota—Drug Interaction |
| title_sort | metabolites analysis of anti myocardial ischemia active components of i saussurea involucrata i based on gut microbiota drug interaction |
| topic | <i>Saussurea involucrata</i> gut microbiota <i>Tian-Shan-Xue-Lian</i> LC-MS/MS LC/MS<sup>n</sup>-IT-TOF metabolite |
| url | https://www.mdpi.com/1422-0067/23/13/7457 |
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