Development of Schistosoma mansoni worms in mice analyzed by bright field and confocal microscopy
The blood flukes of mammals (Digenea: Schistosomatidae) are among trematodes unique whose adult worms have separeted sexes which are dissimilar in appearance. The developmental features, growth and organogenesis of Schistosoma mansoni were studied in Swiss Webster mice by a digital system for image...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2006-10-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900040 |
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| author | Carla de Lamare Biolchini Renata Heisler Neves Maarten Hulstijn Delir Corrêa Gomes José Roberto Machado-Silva |
| author_facet | Carla de Lamare Biolchini Renata Heisler Neves Maarten Hulstijn Delir Corrêa Gomes José Roberto Machado-Silva |
| author_sort | Carla de Lamare Biolchini |
| collection | DOAJ |
| description | The blood flukes of mammals (Digenea: Schistosomatidae) are among trematodes unique whose adult worms have separeted sexes which are dissimilar in appearance. The developmental features, growth and organogenesis of Schistosoma mansoni were studied in Swiss Webster mice by a digital system for image analysis and confocal microscopy. Data so far obtained showed two phases with significative morphological changes at 3-4 weeks post-infection, and a gradual similar development onwards in the reproductive system and tegument. Our male-dependent phase demonstrated that mating occurs before sexual maturing. At week three, the majority of male worms (59%) had formed the gynaecophoric canal although testicular lobes and tegumental tubercles were absent. By this time, 33% females had an incipient ovary (without cellular differentiation). At week four, 77.2% males presented testicular lobes with few germinative cells while 26% had developing tegumental tubercles. The immature ovary was observed in 69% females. Suckers followed different pattern of growth between male and females. The size of oral and ventral suckers from six-week-old male worms grew abruptly (3.0 fold) more than that of three-week-old. In female worms, maximum growth was attained at week four, reducing in size thereafter. From sixth week onwards, all specimens showed the fully developed reproductive system. Probably, these features are morphological traits which schistosome has experienced from hermaphrodite to dioecy. |
| first_indexed | 2024-03-12T18:22:05Z |
| format | Article |
| id | doaj.art-5e7133d862474c3f9ffbea121f615fb2 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:22:05Z |
| publishDate | 2006-10-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-5e7133d862474c3f9ffbea121f615fb22023-08-02T08:46:56ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602006-10-0110126126510.1590/S0074-02762006000900040Development of Schistosoma mansoni worms in mice analyzed by bright field and confocal microscopyCarla de Lamare BiolchiniRenata Heisler NevesMaarten HulstijnDelir Corrêa GomesJosé Roberto Machado-SilvaThe blood flukes of mammals (Digenea: Schistosomatidae) are among trematodes unique whose adult worms have separeted sexes which are dissimilar in appearance. The developmental features, growth and organogenesis of Schistosoma mansoni were studied in Swiss Webster mice by a digital system for image analysis and confocal microscopy. Data so far obtained showed two phases with significative morphological changes at 3-4 weeks post-infection, and a gradual similar development onwards in the reproductive system and tegument. Our male-dependent phase demonstrated that mating occurs before sexual maturing. At week three, the majority of male worms (59%) had formed the gynaecophoric canal although testicular lobes and tegumental tubercles were absent. By this time, 33% females had an incipient ovary (without cellular differentiation). At week four, 77.2% males presented testicular lobes with few germinative cells while 26% had developing tegumental tubercles. The immature ovary was observed in 69% females. Suckers followed different pattern of growth between male and females. The size of oral and ventral suckers from six-week-old male worms grew abruptly (3.0 fold) more than that of three-week-old. In female worms, maximum growth was attained at week four, reducing in size thereafter. From sixth week onwards, all specimens showed the fully developed reproductive system. Probably, these features are morphological traits which schistosome has experienced from hermaphrodite to dioecy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900040DigeneaSchistosoma mansonidevelopment |
| spellingShingle | Carla de Lamare Biolchini Renata Heisler Neves Maarten Hulstijn Delir Corrêa Gomes José Roberto Machado-Silva Development of Schistosoma mansoni worms in mice analyzed by bright field and confocal microscopy Memorias do Instituto Oswaldo Cruz Digenea Schistosoma mansoni development |
| title | Development of Schistosoma mansoni worms in mice analyzed by bright field and confocal microscopy |
| title_full | Development of Schistosoma mansoni worms in mice analyzed by bright field and confocal microscopy |
| title_fullStr | Development of Schistosoma mansoni worms in mice analyzed by bright field and confocal microscopy |
| title_full_unstemmed | Development of Schistosoma mansoni worms in mice analyzed by bright field and confocal microscopy |
| title_short | Development of Schistosoma mansoni worms in mice analyzed by bright field and confocal microscopy |
| title_sort | development of schistosoma mansoni worms in mice analyzed by bright field and confocal microscopy |
| topic | Digenea Schistosoma mansoni development |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000900040 |
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