Protoplast as a tool to address questions in plant physiology
A protoplast is a plant cell from which the cell wall has been removed by enzyme treatment. Tremendous utilization of protoplast in molecular analysis of plant growth and development has been observed in the past decades and the system has paved its way to significantly facilitate the comprehensive...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Alexandru Ioan Cuza University of Iasi
2018-12-01
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| Series: | Journal of Plant Development |
| Subjects: | |
| Online Access: | http://www.plant-journal.uaic.ro/docs/2018/9.pdf |
| _version_ | 1811316087696916480 |
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| author | Tim XING Lining TIAN |
| author_facet | Tim XING Lining TIAN |
| author_sort | Tim XING |
| collection | DOAJ |
| description | A protoplast is a plant cell from which the cell wall has been removed by enzyme treatment. Tremendous utilization of protoplast in molecular analysis of plant growth and development has been observed in the past decades and the system has paved its way to significantly facilitate the comprehensive understanding of the complexity of underlying mechanisms. However, it should be kept in mind that a plant, like all systems, is composed of networks of interdependent components that integrate the system into a unified whole. In this mini review, we will re-explore protoplast approach in answering plant physiology questions through discussion of its application in the study of (1) photosynthesis and chloroplast-related process; (2) pollen tube growth; (3) sieve tube element protoplast for long distance translocation; (4) new regulatory metabolites from guard cell protoplast. This in vitro approach may open the way to further meaningful results at organismal level. |
| first_indexed | 2024-04-13T11:42:40Z |
| format | Article |
| id | doaj.art-5e7b03c07921473db3f57cecbaab99cb |
| institution | Directory Open Access Journal |
| issn | 2065-3158 2066-9917 |
| language | English |
| last_indexed | 2024-04-13T11:42:40Z |
| publishDate | 2018-12-01 |
| publisher | Alexandru Ioan Cuza University of Iasi |
| record_format | Article |
| series | Journal of Plant Development |
| spelling | doaj.art-5e7b03c07921473db3f57cecbaab99cb2022-12-22T02:48:16ZengAlexandru Ioan Cuza University of IasiJournal of Plant Development2065-31582066-99172018-12-01259910510.33628/jpd.2018.25.1.99Protoplast as a tool to address questions in plant physiologyTim XING0Lining TIAN1Department of Biology and Institute of Biochemistry, Carleton University, Ottawa–Canada K1S 5B6Agriculture and Agri-Food Canada, London Research and Development Centre, London, ON – Canada N5V 4T3A protoplast is a plant cell from which the cell wall has been removed by enzyme treatment. Tremendous utilization of protoplast in molecular analysis of plant growth and development has been observed in the past decades and the system has paved its way to significantly facilitate the comprehensive understanding of the complexity of underlying mechanisms. However, it should be kept in mind that a plant, like all systems, is composed of networks of interdependent components that integrate the system into a unified whole. In this mini review, we will re-explore protoplast approach in answering plant physiology questions through discussion of its application in the study of (1) photosynthesis and chloroplast-related process; (2) pollen tube growth; (3) sieve tube element protoplast for long distance translocation; (4) new regulatory metabolites from guard cell protoplast. This in vitro approach may open the way to further meaningful results at organismal level.http://www.plant-journal.uaic.ro/docs/2018/9.pdfguard cellpollen tubeprotoplastphotosynthesissieve tube elementstomata |
| spellingShingle | Tim XING Lining TIAN Protoplast as a tool to address questions in plant physiology Journal of Plant Development guard cell pollen tube protoplast photosynthesis sieve tube element stomata |
| title | Protoplast as a tool to address questions in plant physiology |
| title_full | Protoplast as a tool to address questions in plant physiology |
| title_fullStr | Protoplast as a tool to address questions in plant physiology |
| title_full_unstemmed | Protoplast as a tool to address questions in plant physiology |
| title_short | Protoplast as a tool to address questions in plant physiology |
| title_sort | protoplast as a tool to address questions in plant physiology |
| topic | guard cell pollen tube protoplast photosynthesis sieve tube element stomata |
| url | http://www.plant-journal.uaic.ro/docs/2018/9.pdf |
| work_keys_str_mv | AT timxing protoplastasatooltoaddressquestionsinplantphysiology AT liningtian protoplastasatooltoaddressquestionsinplantphysiology |