Potent antibacterial activity of Terminalia chebula-based herbal soap formulation against propionibacterium acnes and its cytotoxic evaluation on human skin fibroblast cells
Background: Recently, many approaches have been conducted in natural skin healthcare, with herbal remedies gaining prominence due to their perceived efficacy and fewer side effects. Herbal soaps, in particular, are noted for their antimicrobial, anti-inflammatory, and antioxidant properties, making...
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| Format: | Article |
| Language: | English |
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Creative Pharma Assent
2023-12-01
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| Series: | Journal of Applied Pharmaceutical Research |
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| Online Access: | https://japtronline.com/index.php/joapr/article/view/384 |
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| author | Mukhtar Almukashir Shamsi Saad Shamsi Salahaldin Alfurjany Enas Ali Heba Zidan |
| author_facet | Mukhtar Almukashir Shamsi Saad Shamsi Salahaldin Alfurjany Enas Ali Heba Zidan |
| author_sort | Mukhtar Almukashir |
| collection | DOAJ |
| description | Background: Recently, many approaches have been conducted in natural skin healthcare, with herbal remedies gaining prominence due to their perceived efficacy and fewer side effects. Herbal soaps, in particular, are noted for their antimicrobial, anti-inflammatory, and antioxidant properties, making them appealing for acne-causing bacteria such as Propionibacterium acnes, Terminalia chebula, a medicinal plant with a rich history of traditional medicinal properties, including antibacterial effects. However, limited information exists on its activity against acne-causing bacteria and its cytotoxicity on human skin cells. Methods: Our study demonstrated the anti-propionibacterium activity using a novel Terminalia chebula herbal soap formulation (TC-HSF), and to determine its cytotoxicity on Human skin fibroblast cells, Terminalia chebula aqueous extract was used to make herbal soap, the antibacterial activity was performed against wild isolated propionibacterium acne strain using well-diffusion method, MIC and time-kill Kinetics, several concentrations were evaluated for cytotoxicity on Human skin fibroblast cell lines.
Results Our findings indicate a potent activity of TC-HSF against P.acne at 0.18mg\ml with lower CFU\ml in tested intervals and high cell viability 98%, IC50 Value was 50mg\ml. Conclusion: Our study highlights the promising antibacterial efficacy of TC-HSF against Propionibacterium acnes and its cytotoxic effects on human skin fibroblast cells. The research adds valuable insights into the potential of TC-HSF for acne management and underscores the importance of dosage considerations in the formulation of herbal soap products. |
| first_indexed | 2024-03-08T12:48:18Z |
| format | Article |
| id | doaj.art-5e7bfd63cafb497fb5ee9ae17af3bf86 |
| institution | Directory Open Access Journal |
| issn | 2348-0335 |
| language | English |
| last_indexed | 2024-03-08T12:48:18Z |
| publishDate | 2023-12-01 |
| publisher | Creative Pharma Assent |
| record_format | Article |
| series | Journal of Applied Pharmaceutical Research |
| spelling | doaj.art-5e7bfd63cafb497fb5ee9ae17af3bf862024-01-21T03:00:26ZengCreative Pharma AssentJournal of Applied Pharmaceutical Research2348-03352023-12-01115010810.18231/j.joapr.2023.11.5.1.8385Potent antibacterial activity of Terminalia chebula-based herbal soap formulation against propionibacterium acnes and its cytotoxic evaluation on human skin fibroblast cellsMukhtar Almukashir0Shamsi Saad Shamsi1Salahaldin Alfurjany2Enas Ali3Heba Zidan4Department of Botany, Research Laboratory, Faculty of Science, Sebha University, SebhaDepartment of Microbiology, Libyan Medical Research Center, Sebha Department of Microbiology, Faculty of Science, Sebha University, SebhaDepartment of Botany, Research Laboratory, Faculty of Science, Sebha University, SebhaDepartment of Microbiology, Libyan Medical Research Center, SebhaDepartment of Microbiology, Libyan Medical Research Center, SebhaBackground: Recently, many approaches have been conducted in natural skin healthcare, with herbal remedies gaining prominence due to their perceived efficacy and fewer side effects. Herbal soaps, in particular, are noted for their antimicrobial, anti-inflammatory, and antioxidant properties, making them appealing for acne-causing bacteria such as Propionibacterium acnes, Terminalia chebula, a medicinal plant with a rich history of traditional medicinal properties, including antibacterial effects. However, limited information exists on its activity against acne-causing bacteria and its cytotoxicity on human skin cells. Methods: Our study demonstrated the anti-propionibacterium activity using a novel Terminalia chebula herbal soap formulation (TC-HSF), and to determine its cytotoxicity on Human skin fibroblast cells, Terminalia chebula aqueous extract was used to make herbal soap, the antibacterial activity was performed against wild isolated propionibacterium acne strain using well-diffusion method, MIC and time-kill Kinetics, several concentrations were evaluated for cytotoxicity on Human skin fibroblast cell lines. Results Our findings indicate a potent activity of TC-HSF against P.acne at 0.18mg\ml with lower CFU\ml in tested intervals and high cell viability 98%, IC50 Value was 50mg\ml. Conclusion: Our study highlights the promising antibacterial efficacy of TC-HSF against Propionibacterium acnes and its cytotoxic effects on human skin fibroblast cells. The research adds valuable insights into the potential of TC-HSF for acne management and underscores the importance of dosage considerations in the formulation of herbal soap products.https://japtronline.com/index.php/joapr/article/view/384terminalia chebula herbal soappropinobacteriumhuman skin fibroblast cells |
| spellingShingle | Mukhtar Almukashir Shamsi Saad Shamsi Salahaldin Alfurjany Enas Ali Heba Zidan Potent antibacterial activity of Terminalia chebula-based herbal soap formulation against propionibacterium acnes and its cytotoxic evaluation on human skin fibroblast cells Journal of Applied Pharmaceutical Research terminalia chebula herbal soap propinobacterium human skin fibroblast cells |
| title | Potent antibacterial activity of Terminalia chebula-based herbal soap formulation against propionibacterium acnes and its cytotoxic evaluation on human skin fibroblast cells |
| title_full | Potent antibacterial activity of Terminalia chebula-based herbal soap formulation against propionibacterium acnes and its cytotoxic evaluation on human skin fibroblast cells |
| title_fullStr | Potent antibacterial activity of Terminalia chebula-based herbal soap formulation against propionibacterium acnes and its cytotoxic evaluation on human skin fibroblast cells |
| title_full_unstemmed | Potent antibacterial activity of Terminalia chebula-based herbal soap formulation against propionibacterium acnes and its cytotoxic evaluation on human skin fibroblast cells |
| title_short | Potent antibacterial activity of Terminalia chebula-based herbal soap formulation against propionibacterium acnes and its cytotoxic evaluation on human skin fibroblast cells |
| title_sort | potent antibacterial activity of terminalia chebula based herbal soap formulation against propionibacterium acnes and its cytotoxic evaluation on human skin fibroblast cells |
| topic | terminalia chebula herbal soap propinobacterium human skin fibroblast cells |
| url | https://japtronline.com/index.php/joapr/article/view/384 |
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