Investigating Buffer Effects on Lysozyme Adsorption to Borosilicate Glass
Protein adsorption refers to the accumulation and adherence of a protein to the surface of a solid, but without surface, penetration occurring. Proteins can adsorb to a variety of interfaces that are used in the manufacture, formulation, and storage of protein medicines. This can have unintended c...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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University of Huddersfield Press
2022-11-01
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| Series: | British Journal of Pharmacy |
| Subjects: | |
| Online Access: | https://www.bjpharm.org.uk/article/id/1157/ |
| _version_ | 1797825313424539648 |
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| author | John Donal Downey Abina Crean Katie B Ryan |
| author_facet | John Donal Downey Abina Crean Katie B Ryan |
| author_sort | John Donal Downey |
| collection | DOAJ |
| description |
Protein adsorption refers to the accumulation and adherence of a protein to the surface of a solid, but without surface, penetration occurring. Proteins can adsorb to a variety of interfaces that are used in the manufacture, formulation, and storage of protein medicines. This can have unintended consequences such as a loss of expensive protein products and aggregate formation. This study investigates the role of buffer composition, pH, and protein concentration on the adsorption of lysozyme to borosilicate glass. Using reverse-phase HPLC and differential scanning fluorimetry, we quantified the mass of adsorbed lysozyme and assessed the stability of lysozyme in each buffer system. The highest amount of adsorbed lysozyme occurred in the sodium phosphate and histidine-HCl buffers at pH 7.4 and stability analysis showed that lysozyme had the lowest melt temperature in these buffers. |
| first_indexed | 2024-03-13T10:52:16Z |
| format | Article |
| id | doaj.art-5e7ce2051f6b4524ab53b0ad9438a037 |
| institution | Directory Open Access Journal |
| issn | 2058-8356 |
| language | English |
| last_indexed | 2024-03-13T10:52:16Z |
| publishDate | 2022-11-01 |
| publisher | University of Huddersfield Press |
| record_format | Article |
| series | British Journal of Pharmacy |
| spelling | doaj.art-5e7ce2051f6b4524ab53b0ad9438a0372023-05-17T10:20:32ZengUniversity of Huddersfield PressBritish Journal of Pharmacy2058-83562022-11-017210.5920/bjpharm.1157Investigating Buffer Effects on Lysozyme Adsorption to Borosilicate GlassJohn Donal Downey0Abina Crean1Katie B Ryan2University College CorkUniversity College CorkUniversity College Cork Protein adsorption refers to the accumulation and adherence of a protein to the surface of a solid, but without surface, penetration occurring. Proteins can adsorb to a variety of interfaces that are used in the manufacture, formulation, and storage of protein medicines. This can have unintended consequences such as a loss of expensive protein products and aggregate formation. This study investigates the role of buffer composition, pH, and protein concentration on the adsorption of lysozyme to borosilicate glass. Using reverse-phase HPLC and differential scanning fluorimetry, we quantified the mass of adsorbed lysozyme and assessed the stability of lysozyme in each buffer system. The highest amount of adsorbed lysozyme occurred in the sodium phosphate and histidine-HCl buffers at pH 7.4 and stability analysis showed that lysozyme had the lowest melt temperature in these buffers. https://www.bjpharm.org.uk/article/id/1157/protein adsorptionbufferstabilityborosilicate |
| spellingShingle | John Donal Downey Abina Crean Katie B Ryan Investigating Buffer Effects on Lysozyme Adsorption to Borosilicate Glass British Journal of Pharmacy protein adsorption buffer stability borosilicate |
| title | Investigating Buffer Effects on Lysozyme Adsorption to Borosilicate Glass |
| title_full | Investigating Buffer Effects on Lysozyme Adsorption to Borosilicate Glass |
| title_fullStr | Investigating Buffer Effects on Lysozyme Adsorption to Borosilicate Glass |
| title_full_unstemmed | Investigating Buffer Effects on Lysozyme Adsorption to Borosilicate Glass |
| title_short | Investigating Buffer Effects on Lysozyme Adsorption to Borosilicate Glass |
| title_sort | investigating buffer effects on lysozyme adsorption to borosilicate glass |
| topic | protein adsorption buffer stability borosilicate |
| url | https://www.bjpharm.org.uk/article/id/1157/ |
| work_keys_str_mv | AT johndonaldowney investigatingbuffereffectsonlysozymeadsorptiontoborosilicateglass AT abinacrean investigatingbuffereffectsonlysozymeadsorptiontoborosilicateglass AT katiebryan investigatingbuffereffectsonlysozymeadsorptiontoborosilicateglass |