Methamphetamine-induced conditioned place preference in LG/J and SM/J mouse strains and an F45/F46 advanced intercross line
The conditioned place preference (CPP) test is frequently used to evaluate the rewarding properties of drugs of abuse in mice. Despite its widespread use in transgenic and knockout experiments, there are few forward genetic studies using CPP to identify novel genes contributing to drug reward. In...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2012-07-01
|
| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fgene.2012.00126/full |
| _version_ | 1819170670094843904 |
|---|---|
| author | Camron D Bryant Loren A Kole Michael A Guido Riyan eCheng Abraham A Palmer Abraham A Palmer |
| author_facet | Camron D Bryant Loren A Kole Michael A Guido Riyan eCheng Abraham A Palmer Abraham A Palmer |
| author_sort | Camron D Bryant |
| collection | DOAJ |
| description | The conditioned place preference (CPP) test is frequently used to evaluate the rewarding properties of drugs of abuse in mice. Despite its widespread use in transgenic and knockout experiments, there are few forward genetic studies using CPP to identify novel genes contributing to drug reward. In this study, we tested LG/J and SM/J inbred strains and the parents/offspring of 10 families of an F45/F46 advanced intercross line (AIL) for methamphetamine-induced CPP (MA-CPP) once per week over two weeks. Both LG/J and SM/J mice exhibited significant MA-CPP that was not significantly different between the two strains. Furthermore, LG/J mice showed significantly less acute MA-induced locomotor activity as well as locomotor sensitization following subsequent MA injections. AIL mice (N = 105) segregating LG/J and SM/J alleles also demonstrated significant MA-CPP that was equal in magnitude between the first and second week of training. Importantly, MA-CPP in AIL mice did not correlate with drug-free or MA-induced locomotor activity, indicating that MA-CPP was not confounded by test session activity and implying that MA-CPP is genetically distinct from acute psychomotor sensitivity. We estimated the heritability of MA-CPP and locomotor phenotypes using midparent-offspring regression and maximum likelihood estimates derived from the kinship coefficients of the AIL pedigree. Heritability estimates of MA-CPP were low (0-0.21) and variable (S.E. = 0-0.33) which reflected our poor power to estimate heritability using only 10 midparent-offspring observations. In sum, we established a short-term protocol for MA-CPP in AIL mice that could reveal LG/J and SM/J alleles important for MA reward. The use of highly recombinant genetic populations like AIL should facilitate the identification of these genes and may have implications for understanding psychostimulant abuse in humans. |
| first_indexed | 2024-12-22T19:39:05Z |
| format | Article |
| id | doaj.art-5e807e8120a741018da9b3f8412d4d1d |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-22T19:39:05Z |
| publishDate | 2012-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-5e807e8120a741018da9b3f8412d4d1d2022-12-21T18:14:55ZengFrontiers Media S.A.Frontiers in Genetics1664-80212012-07-01310.3389/fgene.2012.0012627185Methamphetamine-induced conditioned place preference in LG/J and SM/J mouse strains and an F45/F46 advanced intercross lineCamron D Bryant0Loren A Kole1Michael A Guido2Riyan eCheng3Abraham A Palmer4Abraham A Palmer5The University of ChicagoThe University of ChicagoThe University of ChicagoThe University of ChicagoThe University of ChicagoThe University of ChicagoThe conditioned place preference (CPP) test is frequently used to evaluate the rewarding properties of drugs of abuse in mice. Despite its widespread use in transgenic and knockout experiments, there are few forward genetic studies using CPP to identify novel genes contributing to drug reward. In this study, we tested LG/J and SM/J inbred strains and the parents/offspring of 10 families of an F45/F46 advanced intercross line (AIL) for methamphetamine-induced CPP (MA-CPP) once per week over two weeks. Both LG/J and SM/J mice exhibited significant MA-CPP that was not significantly different between the two strains. Furthermore, LG/J mice showed significantly less acute MA-induced locomotor activity as well as locomotor sensitization following subsequent MA injections. AIL mice (N = 105) segregating LG/J and SM/J alleles also demonstrated significant MA-CPP that was equal in magnitude between the first and second week of training. Importantly, MA-CPP in AIL mice did not correlate with drug-free or MA-induced locomotor activity, indicating that MA-CPP was not confounded by test session activity and implying that MA-CPP is genetically distinct from acute psychomotor sensitivity. We estimated the heritability of MA-CPP and locomotor phenotypes using midparent-offspring regression and maximum likelihood estimates derived from the kinship coefficients of the AIL pedigree. Heritability estimates of MA-CPP were low (0-0.21) and variable (S.E. = 0-0.33) which reflected our poor power to estimate heritability using only 10 midparent-offspring observations. In sum, we established a short-term protocol for MA-CPP in AIL mice that could reveal LG/J and SM/J alleles important for MA reward. The use of highly recombinant genetic populations like AIL should facilitate the identification of these genes and may have implications for understanding psychostimulant abuse in humans.http://journal.frontiersin.org/Journal/10.3389/fgene.2012.00126/fullAmphetamineLocomotionAddictionReinforcementDrug abusePsychostimulants |
| spellingShingle | Camron D Bryant Loren A Kole Michael A Guido Riyan eCheng Abraham A Palmer Abraham A Palmer Methamphetamine-induced conditioned place preference in LG/J and SM/J mouse strains and an F45/F46 advanced intercross line Frontiers in Genetics Amphetamine Locomotion Addiction Reinforcement Drug abuse Psychostimulants |
| title | Methamphetamine-induced conditioned place preference in LG/J and SM/J mouse strains and an F45/F46 advanced intercross line |
| title_full | Methamphetamine-induced conditioned place preference in LG/J and SM/J mouse strains and an F45/F46 advanced intercross line |
| title_fullStr | Methamphetamine-induced conditioned place preference in LG/J and SM/J mouse strains and an F45/F46 advanced intercross line |
| title_full_unstemmed | Methamphetamine-induced conditioned place preference in LG/J and SM/J mouse strains and an F45/F46 advanced intercross line |
| title_short | Methamphetamine-induced conditioned place preference in LG/J and SM/J mouse strains and an F45/F46 advanced intercross line |
| title_sort | methamphetamine induced conditioned place preference in lg j and sm j mouse strains and an f45 f46 advanced intercross line |
| topic | Amphetamine Locomotion Addiction Reinforcement Drug abuse Psychostimulants |
| url | http://journal.frontiersin.org/Journal/10.3389/fgene.2012.00126/full |
| work_keys_str_mv | AT camrondbryant methamphetamineinducedconditionedplacepreferenceinlgjandsmjmousestrainsandanf45f46advancedintercrossline AT lorenakole methamphetamineinducedconditionedplacepreferenceinlgjandsmjmousestrainsandanf45f46advancedintercrossline AT michaelaguido methamphetamineinducedconditionedplacepreferenceinlgjandsmjmousestrainsandanf45f46advancedintercrossline AT riyanecheng methamphetamineinducedconditionedplacepreferenceinlgjandsmjmousestrainsandanf45f46advancedintercrossline AT abrahamapalmer methamphetamineinducedconditionedplacepreferenceinlgjandsmjmousestrainsandanf45f46advancedintercrossline AT abrahamapalmer methamphetamineinducedconditionedplacepreferenceinlgjandsmjmousestrainsandanf45f46advancedintercrossline |