Use of alternative extender added of fructose aiming the cryopreservation of boar semen
This study aimed to analyze skimmed milk powder (SMP) and fructose in a new cooling curve to freeze boar semen. A total of 49 semen samples from seven boars were cryopreserved using the new curve with addition of glucose and fructose to the refrigerating diluents: Beltsville Thawing Solution (BTS +...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2018-04-01
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| Series: | Brazilian Journal of Veterinary Research and Animal Science |
| Subjects: | |
| Online Access: | http://www.revistas.usp.br/bjvras/article/view/115739 |
| _version_ | 1818177510788038656 |
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| author | Ludymila Furtado Cantanhêde Eduardo Nunes de Freitas Tatyane Bandeira Barros Daianny Barboza Guimarães Aline Viana Dias Ricardo Toniolli |
| author_facet | Ludymila Furtado Cantanhêde Eduardo Nunes de Freitas Tatyane Bandeira Barros Daianny Barboza Guimarães Aline Viana Dias Ricardo Toniolli |
| author_sort | Ludymila Furtado Cantanhêde |
| collection | DOAJ |
| description | This study aimed to analyze skimmed milk powder (SMP) and fructose in a new cooling curve to freeze boar semen. A total of 49 semen samples from seven boars were cryopreserved using the new curve with addition of glucose and fructose to the refrigerating diluents: Beltsville Thawing Solution (BTS + G; BTS + F) and Skimmed milk powder (SMP + G; SMP + F), totaling four experimental groups for analysis. To finish the curve, aliquots of semen were packaged in 0.5 ml straws and kept in liquid nitrogen. During the cooling curve, SMP mean spermatic vigor and motility were greater than the BTS (p < 0.05). After thawing, a decrease of spermatic force and motility in both extenders was observed, where the BTS presented spermatic vigor (2.1 ± 0.55) and motility (38 ± 21.8), presenting better results (p < 0.05). There was no statistical difference between sugars added to the BTS and SMP in spermatic force and motility (p > 0.05), although the use of fructose allowed an equalization of motility between the SMP and BTS (p > 0.05). Functionality of membrane was better preserved with the addition of fructose, in both extenders. The rate of sperm viability was significantly higher in extender containing glucose and SMP (71.8 ± 12.5). The percentage of intact acrosome was higher on the treatment containing glucose, independent of the extender (BTS + G: 81.8 ± 7.2, SMP + G: 81.4 ± 14.2). To conclude, the results suggest that the BTS is still the best option to cryopreserve and fructose could be used in boar semen cryopreservation in new cooling curve. |
| first_indexed | 2024-12-11T20:33:14Z |
| format | Article |
| id | doaj.art-5e80c700f151476b9d14a97d25ebba03 |
| institution | Directory Open Access Journal |
| issn | 1413-9596 1678-4456 |
| language | English |
| last_indexed | 2024-12-11T20:33:14Z |
| publishDate | 2018-04-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Veterinary Research and Animal Science |
| spelling | doaj.art-5e80c700f151476b9d14a97d25ebba032022-12-22T00:51:44ZengUniversidade de São PauloBrazilian Journal of Veterinary Research and Animal Science1413-95961678-44562018-04-0155110.11606/issn.1678-4456.bjvras.2018.115739Use of alternative extender added of fructose aiming the cryopreservation of boar semenLudymila Furtado Cantanhêde0Eduardo Nunes de Freitas1Tatyane Bandeira Barros2Daianny Barboza Guimarães3Aline Viana Dias4Ricardo Toniolli5Universidade Estadual do Ceará, Faculdade de Veterinária, Programa de Pós-Graduação em Ciências VeterináriasUniversidade Estadual do Ceará, Faculdade de Veterinária, Programa de Pós-Graduação em Ciências VeterináriasUniversidade Estadual do Ceará, Faculdade de Veterinária, Programa de Pós-Graduação em Ciências VeterináriasUniversidade Estadual do Ceará, Faculdade de Veterinária, Programa de Pós-Graduação em Ciências VeterináriasUniversidade Estadual do Ceará, Faculdade de Veterinária, Programa de Pós-Graduação em Ciências VeterináriasUniversidade Estadual do Ceará, Faculdade de Veterinária, Laboratório de Reprodução Suína e Tecnologia de SêmenThis study aimed to analyze skimmed milk powder (SMP) and fructose in a new cooling curve to freeze boar semen. A total of 49 semen samples from seven boars were cryopreserved using the new curve with addition of glucose and fructose to the refrigerating diluents: Beltsville Thawing Solution (BTS + G; BTS + F) and Skimmed milk powder (SMP + G; SMP + F), totaling four experimental groups for analysis. To finish the curve, aliquots of semen were packaged in 0.5 ml straws and kept in liquid nitrogen. During the cooling curve, SMP mean spermatic vigor and motility were greater than the BTS (p < 0.05). After thawing, a decrease of spermatic force and motility in both extenders was observed, where the BTS presented spermatic vigor (2.1 ± 0.55) and motility (38 ± 21.8), presenting better results (p < 0.05). There was no statistical difference between sugars added to the BTS and SMP in spermatic force and motility (p > 0.05), although the use of fructose allowed an equalization of motility between the SMP and BTS (p > 0.05). Functionality of membrane was better preserved with the addition of fructose, in both extenders. The rate of sperm viability was significantly higher in extender containing glucose and SMP (71.8 ± 12.5). The percentage of intact acrosome was higher on the treatment containing glucose, independent of the extender (BTS + G: 81.8 ± 7.2, SMP + G: 81.4 ± 14.2). To conclude, the results suggest that the BTS is still the best option to cryopreserve and fructose could be used in boar semen cryopreservation in new cooling curve.http://www.revistas.usp.br/bjvras/article/view/115739CryopreservationCooling curveSpermAlternative extender |
| spellingShingle | Ludymila Furtado Cantanhêde Eduardo Nunes de Freitas Tatyane Bandeira Barros Daianny Barboza Guimarães Aline Viana Dias Ricardo Toniolli Use of alternative extender added of fructose aiming the cryopreservation of boar semen Brazilian Journal of Veterinary Research and Animal Science Cryopreservation Cooling curve Sperm Alternative extender |
| title | Use of alternative extender added of fructose aiming the cryopreservation of boar semen |
| title_full | Use of alternative extender added of fructose aiming the cryopreservation of boar semen |
| title_fullStr | Use of alternative extender added of fructose aiming the cryopreservation of boar semen |
| title_full_unstemmed | Use of alternative extender added of fructose aiming the cryopreservation of boar semen |
| title_short | Use of alternative extender added of fructose aiming the cryopreservation of boar semen |
| title_sort | use of alternative extender added of fructose aiming the cryopreservation of boar semen |
| topic | Cryopreservation Cooling curve Sperm Alternative extender |
| url | http://www.revistas.usp.br/bjvras/article/view/115739 |
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