Development and clinical validation of a one-step pentaplex real-time reverse transcription PCR assay for detection of hepatitis virus B, C, E, Treponema pallidum, and a human housekeeping gene

Development and clinical validation of a one-step pentaplex real-time reverse transcription PCR assay for detection of hepatitis virus B, C, E, Treponema pallidum, and a human housekeeping gene

Abstract Background With the safety of blood transfusion being a major public health concern, the development of a rapid, sensitive, specific, and cost-effective multiplex PCR assay for simultaneous detection of hepatitis B virus(HBV), hepatitis C virus (HCV), hepatitis E virus (HEV), and Treponema...

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Bibliographic Details
Main Authors: Miaomiao Li, Yan Lv, Dawei Cui, Yushan Xu, Mengjiao Lin, Xiaojun Zhang, Yongjun Wang, Cuifen Shen, Jue Xie
Format: Article
Language:English
Published: BMC 2023-05-01
Series:BMC Infectious Diseases
Subjects:
Blood safety
Hepatitis B Virus
Hepatitis C Virus
Hepatitis E Virus
Treponema pallidum
Pentaplex real-time RT-PCR
Online Access:https://doi.org/10.1186/s12879-023-08240-w

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