Release of airborne antibiotic resistance genes from municipal solid waste transfer stations
Abstract With urbanization, municipal solid waste (MSW) transfer station as an important link in the process of refuse collection and transportation is closer to residents’ living areas. At present, studies on airborne antibiotic resistance genes (ARGs) generated during MSW management are still few...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | Sustainable Environment Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42834-022-00137-8 |
| _version_ | 1811305825193426944 |
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| author | Fan Lü Wei Wang Tianyu Hu Haowen Duan Liming Shao Hua Zhang Pinjing He |
| author_facet | Fan Lü Wei Wang Tianyu Hu Haowen Duan Liming Shao Hua Zhang Pinjing He |
| author_sort | Fan Lü |
| collection | DOAJ |
| description | Abstract With urbanization, municipal solid waste (MSW) transfer station as an important link in the process of refuse collection and transportation is closer to residents’ living areas. At present, studies on airborne antibiotic resistance genes (ARGs) generated during MSW management are still few and not comprehensive enough. In this study, metagenomics and high-throughput sequencing methods were used to analyze the composition and content of bioaerosol and airborne ARGs in the waste reception hall and the top vent of three MSW transfer stations. 265 ARG subtypes were detected. The main subtypes were bacA, multidrug_transporter, mexW, sul2 and macB, and the main types were multidrug resistance genes and bacitracin resistance genes. Different microbes and ARGs showed diverse release characteristics, but in general, the concentration of bacteria and ARGs at the top vent of the transfer station was higher than that in the waste reception hall, and fungi were more sensitive than bacteria when passing through the odor treatment system connecting between the top vent and the waste reception hall. For ARGs, daily intake of a worker was calculated to be 1.08 × 1010–2.79 × 1010 copies d− 1 and environmental release was 2.88 × 109–9.49 × 109 copies m− 3. Therefore, control measures for airborne ARGs and pathogenic microorganisms are urgently needed to ensure the health of workers and surrounding residents. |
| first_indexed | 2024-04-13T08:33:20Z |
| format | Article |
| id | doaj.art-5e84244a66dc4c0d81445a03b991f377 |
| institution | Directory Open Access Journal |
| issn | 2468-2039 |
| language | English |
| last_indexed | 2024-04-13T08:33:20Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Sustainable Environment Research |
| spelling | doaj.art-5e84244a66dc4c0d81445a03b991f3772022-12-22T02:54:11ZengBMCSustainable Environment Research2468-20392022-05-0132111310.1186/s42834-022-00137-8Release of airborne antibiotic resistance genes from municipal solid waste transfer stationsFan Lü0Wei Wang1Tianyu Hu2Haowen Duan3Liming Shao4Hua Zhang5Pinjing He6State Key Laboratory of Pollution Control and Resource Reuse, Tongji UniversityState Key Laboratory of Pollution Control and Resource Reuse, Tongji UniversityState Key Laboratory of Pollution Control and Resource Reuse, Tongji UniversityState Key Laboratory of Pollution Control and Resource Reuse, Tongji UniversityInstitute of Waste Treatment and Reclamation, Tongji UniversityState Key Laboratory of Pollution Control and Resource Reuse, Tongji UniversityInstitute of Waste Treatment and Reclamation, Tongji UniversityAbstract With urbanization, municipal solid waste (MSW) transfer station as an important link in the process of refuse collection and transportation is closer to residents’ living areas. At present, studies on airborne antibiotic resistance genes (ARGs) generated during MSW management are still few and not comprehensive enough. In this study, metagenomics and high-throughput sequencing methods were used to analyze the composition and content of bioaerosol and airborne ARGs in the waste reception hall and the top vent of three MSW transfer stations. 265 ARG subtypes were detected. The main subtypes were bacA, multidrug_transporter, mexW, sul2 and macB, and the main types were multidrug resistance genes and bacitracin resistance genes. Different microbes and ARGs showed diverse release characteristics, but in general, the concentration of bacteria and ARGs at the top vent of the transfer station was higher than that in the waste reception hall, and fungi were more sensitive than bacteria when passing through the odor treatment system connecting between the top vent and the waste reception hall. For ARGs, daily intake of a worker was calculated to be 1.08 × 1010–2.79 × 1010 copies d− 1 and environmental release was 2.88 × 109–9.49 × 109 copies m− 3. Therefore, control measures for airborne ARGs and pathogenic microorganisms are urgently needed to ensure the health of workers and surrounding residents.https://doi.org/10.1186/s42834-022-00137-8MSW collection & transportationAirborne antibiotic resistance genesBioaerosolDaily intakeMetagenomicsHigh-throughput sequencing |
| spellingShingle | Fan Lü Wei Wang Tianyu Hu Haowen Duan Liming Shao Hua Zhang Pinjing He Release of airborne antibiotic resistance genes from municipal solid waste transfer stations Sustainable Environment Research MSW collection & transportation Airborne antibiotic resistance genes Bioaerosol Daily intake Metagenomics High-throughput sequencing |
| title | Release of airborne antibiotic resistance genes from municipal solid waste transfer stations |
| title_full | Release of airborne antibiotic resistance genes from municipal solid waste transfer stations |
| title_fullStr | Release of airborne antibiotic resistance genes from municipal solid waste transfer stations |
| title_full_unstemmed | Release of airborne antibiotic resistance genes from municipal solid waste transfer stations |
| title_short | Release of airborne antibiotic resistance genes from municipal solid waste transfer stations |
| title_sort | release of airborne antibiotic resistance genes from municipal solid waste transfer stations |
| topic | MSW collection & transportation Airborne antibiotic resistance genes Bioaerosol Daily intake Metagenomics High-throughput sequencing |
| url | https://doi.org/10.1186/s42834-022-00137-8 |
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