INFLUENCE OF PHYSICOCHEMICAL FACTORS ON DEHYDROGENASE ACTIVITY OF BACILLUS SUBTILIS IMB B-7023
Aim. The investigation of the influence of some physicochemical factors on dehydrogenase activity of Bacillus subtilis IMB B-7023. Methods. The bacteria were cultivated in the periodic process. Dehydrogenase activity was evaluated on reduction of 2,3,5-tetrazolium chloride to triphenilphormazane, th...
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| Format: | Article |
| Language: | English |
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Odessa I. I. Mechnikov National University
2015-08-01
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| Series: | Mìkrobìologìâ ì Bìotehnologìâ |
| Subjects: | |
| Online Access: | http://mbt.onu.edu.ua/article/view/48425 |
| _version_ | 1818560009774039040 |
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| author | І. О. Герасименко І. К. Курдиш |
| author_facet | І. О. Герасименко І. К. Курдиш |
| author_sort | І. О. Герасименко |
| collection | DOAJ |
| description | Aim. The investigation of the influence of some physicochemical factors on dehydrogenase activity of Bacillus subtilis IMB B-7023. Methods. The bacteria were cultivated in the periodic process. Dehydrogenase activity was evaluated on reduction of 2,3,5-tetrazolium chloride to triphenilphormazane, the concentration of which was determined by photocolorymetrical methods. Results. It was defined that dehydrogenase activity reached the greatest significance in the given strain at the temperature of 37 °C in the media having pH 7.0. The highest indices of dehydrogenase activity have been obtained in the content of 10 g/l glucose in it. It was determined that ions of Mg2+ and Ca2+ have stimulated dehydrogenase activity of Bacillus subtilis, and ions of Fe3+, on the contrary, suppressed it. Сonclusion. Actual presence of high sensitiveness of the dehydrogenase complex of these bacteria to physicochemical factors of the environment can be used for making up the prognoses of the given strain activity under certain factors of the environment in its use as a complex bacterial preparation in agroecosystems. |
| first_indexed | 2024-12-14T00:32:53Z |
| format | Article |
| id | doaj.art-5e846512f2aa47ab81df9420c9a2c0cc |
| institution | Directory Open Access Journal |
| issn | 2076-0558 2307-4663 |
| language | English |
| last_indexed | 2024-12-14T00:32:53Z |
| publishDate | 2015-08-01 |
| publisher | Odessa I. I. Mechnikov National University |
| record_format | Article |
| series | Mìkrobìologìâ ì Bìotehnologìâ |
| spelling | doaj.art-5e846512f2aa47ab81df9420c9a2c0cc2022-12-21T23:24:46ZengOdessa I. I. Mechnikov National UniversityMìkrobìologìâ ì Bìotehnologìâ2076-05582307-46632015-08-0104(28)616810.18524/2307-4663.2014.4(28).4842548425INFLUENCE OF PHYSICOCHEMICAL FACTORS ON DEHYDROGENASE ACTIVITY OF BACILLUS SUBTILIS IMB B-7023І. О. Герасименко0І. К. Курдиш1Інститут мікробіології і вірусології НАН УкраїниІнститут мікробіології і вірусології НАН УкраїниAim. The investigation of the influence of some physicochemical factors on dehydrogenase activity of Bacillus subtilis IMB B-7023. Methods. The bacteria were cultivated in the periodic process. Dehydrogenase activity was evaluated on reduction of 2,3,5-tetrazolium chloride to triphenilphormazane, the concentration of which was determined by photocolorymetrical methods. Results. It was defined that dehydrogenase activity reached the greatest significance in the given strain at the temperature of 37 °C in the media having pH 7.0. The highest indices of dehydrogenase activity have been obtained in the content of 10 g/l glucose in it. It was determined that ions of Mg2+ and Ca2+ have stimulated dehydrogenase activity of Bacillus subtilis, and ions of Fe3+, on the contrary, suppressed it. Сonclusion. Actual presence of high sensitiveness of the dehydrogenase complex of these bacteria to physicochemical factors of the environment can be used for making up the prognoses of the given strain activity under certain factors of the environment in its use as a complex bacterial preparation in agroecosystems.http://mbt.onu.edu.ua/article/view/48425bacillus subtilisфізико-хімічні факторидегідрогеназна активність |
| spellingShingle | І. О. Герасименко І. К. Курдиш INFLUENCE OF PHYSICOCHEMICAL FACTORS ON DEHYDROGENASE ACTIVITY OF BACILLUS SUBTILIS IMB B-7023 Mìkrobìologìâ ì Bìotehnologìâ bacillus subtilis фізико-хімічні фактори дегідрогеназна активність |
| title | INFLUENCE OF PHYSICOCHEMICAL FACTORS ON DEHYDROGENASE ACTIVITY OF BACILLUS SUBTILIS IMB B-7023 |
| title_full | INFLUENCE OF PHYSICOCHEMICAL FACTORS ON DEHYDROGENASE ACTIVITY OF BACILLUS SUBTILIS IMB B-7023 |
| title_fullStr | INFLUENCE OF PHYSICOCHEMICAL FACTORS ON DEHYDROGENASE ACTIVITY OF BACILLUS SUBTILIS IMB B-7023 |
| title_full_unstemmed | INFLUENCE OF PHYSICOCHEMICAL FACTORS ON DEHYDROGENASE ACTIVITY OF BACILLUS SUBTILIS IMB B-7023 |
| title_short | INFLUENCE OF PHYSICOCHEMICAL FACTORS ON DEHYDROGENASE ACTIVITY OF BACILLUS SUBTILIS IMB B-7023 |
| title_sort | influence of physicochemical factors on dehydrogenase activity of bacillus subtilis imb b 7023 |
| topic | bacillus subtilis фізико-хімічні фактори дегідрогеназна активність |
| url | http://mbt.onu.edu.ua/article/view/48425 |
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