Morphological, molecular, and biological characterization of bulb rot pathogens in stored Lanzhou lily and the in vitro antifungal efficacy of three plant essential oils
Lanzhou lily (Lilium davidii var. willmottiae) is an exclusive sweet lily variety indigenous to China, which is susceptible to bulbous rot caused by fungal infection during storage. This experiment tests the pathogenicity of the pure culture isolated from the diseased tissue was confirmed in accorda...
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1307966/full |
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| author | Chaoqun Liu Yinquan Wang Yinquan Wang Ling Jin Ling Jin Yan Wang Dongling Liu |
| author_facet | Chaoqun Liu Yinquan Wang Yinquan Wang Ling Jin Ling Jin Yan Wang Dongling Liu |
| author_sort | Chaoqun Liu |
| collection | DOAJ |
| description | Lanzhou lily (Lilium davidii var. willmottiae) is an exclusive sweet lily variety indigenous to China, which is susceptible to bulbous rot caused by fungal infection during storage. This experiment tests the pathogenicity of the pure culture isolated from the diseased tissue was confirmed in accordance with Koch's postulates, and the pathomycetes were identified based on their morphological and molecular characteristics. Furthermore, the biological characteristics of the pathogens were investigated, followed by an evaluation of the antifungal effects of three plant essential oils against them. The results showed that two strains of fungi were isolated from Lanzhou lily rot, which were identified as Fusarium oxysporum Schl. and Aspergillus sydowii (Bain. Et sart.). In addition, the pathogenicity of these two strains of fungi was demonstrated that only F. oxysporum induced rot with similar symptoms during the post-harvest storage period. The biological characteristics of F. oxysporum indicated the potato maltose agar and lily dextrose agar were identified as the most suitable media. Sucrose was determined to be the optimal carbon source, while ammonium nitrate was found to be the best nitrogen source for the growth of F. oxysporum. Mycelial growth and sporulation of F. oxysporum occurred at an optimum pH value of 6. Total darkness facilitated mycelial growth and conidial germination. The ideal temperature for growth was found to be 28°C, while relative humidity did not significantly impact mycelial growth; however, a relative humidity of 55% was most favorable for spore production. Among the three essential oils tested, cinnamon essential oil displayed superior antifungal efficacy against F. oxysporum, whereas angelica essential oil and tea tree essential oil also exhibited moderate inhibitory effects against this pathogen. This research provides valuable theoretical insights for disease control during the storage and transportation of Lanzhou lily. |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-24T11:20:14Z |
| publishDate | 2024-04-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-5e8b554b734f48e1bd4a9877cfa2931c2024-04-11T04:47:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-04-011510.3389/fmicb.2024.13079661307966Morphological, molecular, and biological characterization of bulb rot pathogens in stored Lanzhou lily and the in vitro antifungal efficacy of three plant essential oilsChaoqun Liu0Yinquan Wang1Yinquan Wang2Ling Jin3Ling Jin4Yan Wang5Dongling Liu6College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, ChinaCollege of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, ChinaNorthwest Collaborative Innovation Center for Traditional Chinese Medicine Co-Constructed by Gansu Province and MOE of PRC, Lanzhou, ChinaCollege of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, ChinaNorthwest Collaborative Innovation Center for Traditional Chinese Medicine Co-Constructed by Gansu Province and MOE of PRC, Lanzhou, ChinaCollege of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, ChinaCollege of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, ChinaLanzhou lily (Lilium davidii var. willmottiae) is an exclusive sweet lily variety indigenous to China, which is susceptible to bulbous rot caused by fungal infection during storage. This experiment tests the pathogenicity of the pure culture isolated from the diseased tissue was confirmed in accordance with Koch's postulates, and the pathomycetes were identified based on their morphological and molecular characteristics. Furthermore, the biological characteristics of the pathogens were investigated, followed by an evaluation of the antifungal effects of three plant essential oils against them. The results showed that two strains of fungi were isolated from Lanzhou lily rot, which were identified as Fusarium oxysporum Schl. and Aspergillus sydowii (Bain. Et sart.). In addition, the pathogenicity of these two strains of fungi was demonstrated that only F. oxysporum induced rot with similar symptoms during the post-harvest storage period. The biological characteristics of F. oxysporum indicated the potato maltose agar and lily dextrose agar were identified as the most suitable media. Sucrose was determined to be the optimal carbon source, while ammonium nitrate was found to be the best nitrogen source for the growth of F. oxysporum. Mycelial growth and sporulation of F. oxysporum occurred at an optimum pH value of 6. Total darkness facilitated mycelial growth and conidial germination. The ideal temperature for growth was found to be 28°C, while relative humidity did not significantly impact mycelial growth; however, a relative humidity of 55% was most favorable for spore production. Among the three essential oils tested, cinnamon essential oil displayed superior antifungal efficacy against F. oxysporum, whereas angelica essential oil and tea tree essential oil also exhibited moderate inhibitory effects against this pathogen. This research provides valuable theoretical insights for disease control during the storage and transportation of Lanzhou lily.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1307966/fullLilium davidii var. willmottiae (E. H. Wilson) Raffillbulb rot diseaseFusarium oxysporumbiological characteristicsanti-fungal effectplant essential oils |
| spellingShingle | Chaoqun Liu Yinquan Wang Yinquan Wang Ling Jin Ling Jin Yan Wang Dongling Liu Morphological, molecular, and biological characterization of bulb rot pathogens in stored Lanzhou lily and the in vitro antifungal efficacy of three plant essential oils Frontiers in Microbiology Lilium davidii var. willmottiae (E. H. Wilson) Raffill bulb rot disease Fusarium oxysporum biological characteristics anti-fungal effect plant essential oils |
| title | Morphological, molecular, and biological characterization of bulb rot pathogens in stored Lanzhou lily and the in vitro antifungal efficacy of three plant essential oils |
| title_full | Morphological, molecular, and biological characterization of bulb rot pathogens in stored Lanzhou lily and the in vitro antifungal efficacy of three plant essential oils |
| title_fullStr | Morphological, molecular, and biological characterization of bulb rot pathogens in stored Lanzhou lily and the in vitro antifungal efficacy of three plant essential oils |
| title_full_unstemmed | Morphological, molecular, and biological characterization of bulb rot pathogens in stored Lanzhou lily and the in vitro antifungal efficacy of three plant essential oils |
| title_short | Morphological, molecular, and biological characterization of bulb rot pathogens in stored Lanzhou lily and the in vitro antifungal efficacy of three plant essential oils |
| title_sort | morphological molecular and biological characterization of bulb rot pathogens in stored lanzhou lily and the in vitro antifungal efficacy of three plant essential oils |
| topic | Lilium davidii var. willmottiae (E. H. Wilson) Raffill bulb rot disease Fusarium oxysporum biological characteristics anti-fungal effect plant essential oils |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1307966/full |
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