Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis
Variation in DNA methylation enables plants to inherit traits independently of changes to DNA sequence. Here, we have screened an Arabidopsis population of epigenetic recombinant inbred lines (epiRILs) for resistance against Hyaloperonospora arabidopsidis (Hpa). These lines share the same genetic ba...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-01-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/40655 |
| _version_ | 1818028306524536832 |
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| author | Leonardo Furci Ritushree Jain Joost Stassen Oliver Berkowitz James Whelan David Roquis Victoire Baillet Vincent Colot Frank Johannes Jurriaan Ton |
| author_facet | Leonardo Furci Ritushree Jain Joost Stassen Oliver Berkowitz James Whelan David Roquis Victoire Baillet Vincent Colot Frank Johannes Jurriaan Ton |
| author_sort | Leonardo Furci |
| collection | DOAJ |
| description | Variation in DNA methylation enables plants to inherit traits independently of changes to DNA sequence. Here, we have screened an Arabidopsis population of epigenetic recombinant inbred lines (epiRILs) for resistance against Hyaloperonospora arabidopsidis (Hpa). These lines share the same genetic background, but show variation in heritable patterns of DNA methylation. We identified four epigenetic quantitative trait loci (epiQTLs) that provide quantitative resistance without reducing plant growth or resistance to other (a)biotic stresses. Phenotypic characterisation and RNA-sequencing analysis revealed that Hpa-resistant epiRILs are primed to activate defence responses at the relatively early stages of infection. Collectively, our results show that hypomethylation at selected pericentromeric regions is sufficient to provide quantitative disease resistance, which is associated with genome-wide priming of defence-related genes. Based on comparisons of global gene expression and DNA methylation between the wild-type and resistant epiRILs, we discuss mechanisms by which the pericentromeric epiQTLs could regulate the defence-related transcriptome. |
| first_indexed | 2024-12-10T05:01:42Z |
| format | Article |
| id | doaj.art-5e90b98e654b4fb2bbda04254883800c |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T05:01:42Z |
| publishDate | 2019-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5e90b98e654b4fb2bbda04254883800c2022-12-22T02:01:23ZengeLife Sciences Publications LtdeLife2050-084X2019-01-01810.7554/eLife.40655Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in ArabidopsisLeonardo Furci0https://orcid.org/0000-0003-2155-6289Ritushree Jain1Joost Stassen2https://orcid.org/0000-0001-5483-325XOliver Berkowitz3https://orcid.org/0000-0002-7671-6983James Whelan4David Roquis5Victoire Baillet6Vincent Colot7https://orcid.org/0000-0002-6382-1610Frank Johannes8Jurriaan Ton9https://orcid.org/0000-0002-8512-2802P3 Centre for Plant and Soil Biology, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, United KingdomP3 Centre for Plant and Soil Biology, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, United KingdomP3 Centre for Plant and Soil Biology, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, United KingdomDepartment of Animal, Plant and Soil Science, ARC Centre of Excellence in Plant Energy Biology, La Trobe University, Melbourne, AustraliaDepartment of Animal, Plant and Soil Science, ARC Centre of Excellence in Plant Energy Biology, La Trobe University, Melbourne, AustraliaDepartment of Plant Sciences, Technical University of Munich, Freising, Germany; Institute for Advanced Study, Technical University of Munich, Garching, GermanyInstitut de Biologie de l’Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), PSL Université Paris, Paris, FranceInstitut de Biologie de l’Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), PSL Université Paris, Paris, FranceDepartment of Plant Sciences, Technical University of Munich, Freising, Germany; Institute for Advanced Study, Technical University of Munich, Garching, GermanyP3 Centre for Plant and Soil Biology, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, United KingdomVariation in DNA methylation enables plants to inherit traits independently of changes to DNA sequence. Here, we have screened an Arabidopsis population of epigenetic recombinant inbred lines (epiRILs) for resistance against Hyaloperonospora arabidopsidis (Hpa). These lines share the same genetic background, but show variation in heritable patterns of DNA methylation. We identified four epigenetic quantitative trait loci (epiQTLs) that provide quantitative resistance without reducing plant growth or resistance to other (a)biotic stresses. Phenotypic characterisation and RNA-sequencing analysis revealed that Hpa-resistant epiRILs are primed to activate defence responses at the relatively early stages of infection. Collectively, our results show that hypomethylation at selected pericentromeric regions is sufficient to provide quantitative disease resistance, which is associated with genome-wide priming of defence-related genes. Based on comparisons of global gene expression and DNA methylation between the wild-type and resistant epiRILs, we discuss mechanisms by which the pericentromeric epiQTLs could regulate the defence-related transcriptome.https://elifesciences.org/articles/40655Arabidopsisplant immune signallingplant-microbe interactions |
| spellingShingle | Leonardo Furci Ritushree Jain Joost Stassen Oliver Berkowitz James Whelan David Roquis Victoire Baillet Vincent Colot Frank Johannes Jurriaan Ton Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis eLife Arabidopsis plant immune signalling plant-microbe interactions |
| title | Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis |
| title_full | Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis |
| title_fullStr | Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis |
| title_full_unstemmed | Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis |
| title_short | Identification and characterisation of hypomethylated DNA loci controlling quantitative resistance in Arabidopsis |
| title_sort | identification and characterisation of hypomethylated dna loci controlling quantitative resistance in arabidopsis |
| topic | Arabidopsis plant immune signalling plant-microbe interactions |
| url | https://elifesciences.org/articles/40655 |
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