Comprehensive Proteomics Analysis of In Vitro Canine Oviductal Cell-Derived Extracellular Vesicles
Dogs (Canis lupus familiaris) have unique and peculiar reproductive characteristics. While the interplay between in vitro oviductal cell-derived extracellular vesicles (OC-EVs) and cumulus-oocyte complexes in dogs has begun to be elucidated, no study has yet provided extensive information on the bio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/2/573 |
| _version_ | 1797395639425826816 |
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| author | Seok Hee Lee Saúl Lira-Albarrán Islam M Saadeldin |
| author_facet | Seok Hee Lee Saúl Lira-Albarrán Islam M Saadeldin |
| author_sort | Seok Hee Lee |
| collection | DOAJ |
| description | Dogs (Canis lupus familiaris) have unique and peculiar reproductive characteristics. While the interplay between in vitro oviductal cell-derived extracellular vesicles (OC-EVs) and cumulus-oocyte complexes in dogs has begun to be elucidated, no study has yet provided extensive information on the biological content and physiological function of OC-EVs and their role in canine oocyte development. Here, we aimed to provide the first comprehensive proteomic analysis of OC-EVs. We identified 398 proteins as present in all OC-EVs samples. The functional enrichment analysis using Gene Ontology terms and an Ingenuity Pathway Analysis revealed that the identified proteins were involved in several cellular metabolic processes, including translation, synthesis, expression, and protein metabolism. Notably, the proteins were also involved in critical canonical pathways with essential functions in oocyte and embryo development, such as ERK/MAPK, EIF2, PI3K/AKT, and mTOR signaling. These data would be an important resource for studying canine reproductive physiology and establishing a successful in vitro embryo production system in dogs. |
| first_indexed | 2024-03-09T00:37:26Z |
| format | Article |
| id | doaj.art-5e944f6723934b52a1596ced903154dc |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T00:37:26Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-5e944f6723934b52a1596ced903154dc2023-12-11T18:01:49ZengMDPI AGAnimals2076-26152021-02-0111257310.3390/ani11020573Comprehensive Proteomics Analysis of In Vitro Canine Oviductal Cell-Derived Extracellular VesiclesSeok Hee Lee0Saúl Lira-Albarrán1Islam M Saadeldin2Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, CA 94143, USACenter for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, San Francisco, CA 94143, USADepartment of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, EgyptDogs (Canis lupus familiaris) have unique and peculiar reproductive characteristics. While the interplay between in vitro oviductal cell-derived extracellular vesicles (OC-EVs) and cumulus-oocyte complexes in dogs has begun to be elucidated, no study has yet provided extensive information on the biological content and physiological function of OC-EVs and their role in canine oocyte development. Here, we aimed to provide the first comprehensive proteomic analysis of OC-EVs. We identified 398 proteins as present in all OC-EVs samples. The functional enrichment analysis using Gene Ontology terms and an Ingenuity Pathway Analysis revealed that the identified proteins were involved in several cellular metabolic processes, including translation, synthesis, expression, and protein metabolism. Notably, the proteins were also involved in critical canonical pathways with essential functions in oocyte and embryo development, such as ERK/MAPK, EIF2, PI3K/AKT, and mTOR signaling. These data would be an important resource for studying canine reproductive physiology and establishing a successful in vitro embryo production system in dogs.https://www.mdpi.com/2076-2615/11/2/573canine oviductexosomesextracellular vesiclesproteomics |
| spellingShingle | Seok Hee Lee Saúl Lira-Albarrán Islam M Saadeldin Comprehensive Proteomics Analysis of In Vitro Canine Oviductal Cell-Derived Extracellular Vesicles Animals canine oviduct exosomes extracellular vesicles proteomics |
| title | Comprehensive Proteomics Analysis of In Vitro Canine Oviductal Cell-Derived Extracellular Vesicles |
| title_full | Comprehensive Proteomics Analysis of In Vitro Canine Oviductal Cell-Derived Extracellular Vesicles |
| title_fullStr | Comprehensive Proteomics Analysis of In Vitro Canine Oviductal Cell-Derived Extracellular Vesicles |
| title_full_unstemmed | Comprehensive Proteomics Analysis of In Vitro Canine Oviductal Cell-Derived Extracellular Vesicles |
| title_short | Comprehensive Proteomics Analysis of In Vitro Canine Oviductal Cell-Derived Extracellular Vesicles |
| title_sort | comprehensive proteomics analysis of in vitro canine oviductal cell derived extracellular vesicles |
| topic | canine oviduct exosomes extracellular vesicles proteomics |
| url | https://www.mdpi.com/2076-2615/11/2/573 |
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