Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells
Summary: Vascularization is critical for organ homeostasis and function, but cell-based technologies that promote vascular regeneration are limited. This protocol describes steps to generate human pluripotent stem cell (hPSC)-derived vascular progenitors of the mesothelium lineage. This technology h...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-06-01
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| Series: | STAR Protocols |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720300186 |
| _version_ | 1818114952176599040 |
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| author | Luoman Chen Stephen Dalton |
| author_facet | Luoman Chen Stephen Dalton |
| author_sort | Luoman Chen |
| collection | DOAJ |
| description | Summary: Vascularization is critical for organ homeostasis and function, but cell-based technologies that promote vascular regeneration are limited. This protocol describes steps to generate human pluripotent stem cell (hPSC)-derived vascular progenitors of the mesothelium lineage. This technology has several advantages for the generation of vascular cells. First and foremost, MesoT cells are multipotent progenitors that can generate smooth muscle cells and endothelial cells. MesoT cells therefore have potential utility in tissue repair, tissue engineering, and in vascularization of laboratory grown organs.For complete details on the use and execution of this protocol, please refer to Colunga et al. (2019). |
| first_indexed | 2024-12-11T03:58:54Z |
| format | Article |
| id | doaj.art-5e94b442f691464884fa6978538e308f |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-11T03:58:54Z |
| publishDate | 2020-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-5e94b442f691464884fa6978538e308f2022-12-22T01:21:41ZengElsevierSTAR Protocols2666-16672020-06-0111100031Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem CellsLuoman Chen0Stephen Dalton1Center for Molecular Medicine, 325 Riverbend Road, Athens, GA 30605, USA; Department of Biochemistry and Molecular Biology, 325 Riverbend Road, Athens, GA 30605, USA; Corresponding authorCenter for Molecular Medicine, 325 Riverbend Road, Athens, GA 30605, USA; Department of Biochemistry and Molecular Biology, 325 Riverbend Road, Athens, GA 30605, USA; Corresponding authorSummary: Vascularization is critical for organ homeostasis and function, but cell-based technologies that promote vascular regeneration are limited. This protocol describes steps to generate human pluripotent stem cell (hPSC)-derived vascular progenitors of the mesothelium lineage. This technology has several advantages for the generation of vascular cells. First and foremost, MesoT cells are multipotent progenitors that can generate smooth muscle cells and endothelial cells. MesoT cells therefore have potential utility in tissue repair, tissue engineering, and in vascularization of laboratory grown organs.For complete details on the use and execution of this protocol, please refer to Colunga et al. (2019).http://www.sciencedirect.com/science/article/pii/S2666166720300186 |
| spellingShingle | Luoman Chen Stephen Dalton Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells STAR Protocols |
| title | Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells |
| title_full | Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells |
| title_fullStr | Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells |
| title_full_unstemmed | Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells |
| title_short | Multipotent Vascular Progenitor Cells of the Mesothelium Lineage Generated from Human Pluripotent Stem Cells |
| title_sort | multipotent vascular progenitor cells of the mesothelium lineage generated from human pluripotent stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720300186 |
| work_keys_str_mv | AT luomanchen multipotentvascularprogenitorcellsofthemesotheliumlineagegeneratedfromhumanpluripotentstemcells AT stephendalton multipotentvascularprogenitorcellsofthemesotheliumlineagegeneratedfromhumanpluripotentstemcells |