Characterization of a novel Fluoride resistant bacterial isolate and its capability of Fluoride bioremediation
A Gram positive rod shaped bacterium designated as isolate H1 with Fluoride resistance up to 4 g/L sodium fluoride (NaF) in LB (Luria-Bertani) agar was isolated from a ground water sample of Narketpally area, Nalgonda district, Telangana, India. The colonies of isolate H1 were off white in color. Gr...
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AIMS Press
2022-11-01
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| Series: | AIMS Microbiology |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/microbiol.2022031?viewType=HTML |
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| author | M Thirumala E Sai Krishna P Sindhu Priya S Vishnuvardhan Reddy |
| author_facet | M Thirumala E Sai Krishna P Sindhu Priya S Vishnuvardhan Reddy |
| author_sort | M Thirumala |
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| description | A Gram positive rod shaped bacterium designated as isolate H1 with Fluoride resistance up to 4 g/L sodium fluoride (NaF) in LB (Luria-Bertani) agar was isolated from a ground water sample of Narketpally area, Nalgonda district, Telangana, India. The colonies of isolate H1 were off white in color. Growth patterns of isolate H1 were observed at two different concentrations, 100 and 250 ppm, of NaF and also without NaF in the medium. In cases where NaF was present in the media, the lag phases of the growth curves were extended when compared to the absence of NaF. Optimum pH required for the organism's growth was 8. Isolate H1 required a temperature of 37 °C with 150 rpm and 2% NaCl for its optimal growth in the medium without NaF. Meanwhile, isolate H1 could thrive in a diverse pH range, i.e., pH 5–10, and at an NaCl concentration of up to 11% in the medium with NaF. Based on morphological, biochemical and molecular characterization, isolate H1 was identified as belonging to the genus <italic>Bacillus</italic>. It showed 98.47% 16S rDNA gene sequence similarity with <italic>Bacillus australimaris</italic> NH71_1<sup>T</sup>. Isolate H1 showed high fluoride removals of 22.5% and 38.2% with 100 and 250 mg/L of NaF in the LB broth when incubated at pH 8 and a temperature of 37 °C with 150 rpm for 3 day. Hence, this organism could be a promising isolate to apply for defluoridation of ground water in fluoride contaminthe ated areas. |
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| language | English |
| last_indexed | 2024-04-11T00:13:09Z |
| publishDate | 2022-11-01 |
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| series | AIMS Microbiology |
| spelling | doaj.art-5e9594a6a2ad4000b297594e3a43d0bd2023-01-09T05:55:47ZengAIMS PressAIMS Microbiology2471-18882022-11-018447048310.3934/microbiol.2022031Characterization of a novel Fluoride resistant bacterial isolate and its capability of Fluoride bioremediationM Thirumala0E Sai Krishna 1P Sindhu Priya 2 S Vishnuvardhan Reddy31. Microbial Ecology Laboratory, Department of Biochemistry, UCS, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda-508254, Telangana, India1. Microbial Ecology Laboratory, Department of Biochemistry, UCS, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda-508254, Telangana, India 2. Microbztech Labs Pvt. Ltd., Cherlapally, Nalgonda-508001, Telangana, India1. Microbial Ecology Laboratory, Department of Biochemistry, UCS, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda-508254, Telangana, India 2. Microbztech Labs Pvt. Ltd., Cherlapally, Nalgonda-508001, Telangana, India1. Microbial Ecology Laboratory, Department of Biochemistry, UCS, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda-508254, Telangana, India 2. Microbztech Labs Pvt. Ltd., Cherlapally, Nalgonda-508001, Telangana, IndiaA Gram positive rod shaped bacterium designated as isolate H1 with Fluoride resistance up to 4 g/L sodium fluoride (NaF) in LB (Luria-Bertani) agar was isolated from a ground water sample of Narketpally area, Nalgonda district, Telangana, India. The colonies of isolate H1 were off white in color. Growth patterns of isolate H1 were observed at two different concentrations, 100 and 250 ppm, of NaF and also without NaF in the medium. In cases where NaF was present in the media, the lag phases of the growth curves were extended when compared to the absence of NaF. Optimum pH required for the organism's growth was 8. Isolate H1 required a temperature of 37 °C with 150 rpm and 2% NaCl for its optimal growth in the medium without NaF. Meanwhile, isolate H1 could thrive in a diverse pH range, i.e., pH 5–10, and at an NaCl concentration of up to 11% in the medium with NaF. Based on morphological, biochemical and molecular characterization, isolate H1 was identified as belonging to the genus <italic>Bacillus</italic>. It showed 98.47% 16S rDNA gene sequence similarity with <italic>Bacillus australimaris</italic> NH71_1<sup>T</sup>. Isolate H1 showed high fluoride removals of 22.5% and 38.2% with 100 and 250 mg/L of NaF in the LB broth when incubated at pH 8 and a temperature of 37 °C with 150 rpm for 3 day. Hence, this organism could be a promising isolate to apply for defluoridation of ground water in fluoride contaminthe ated areas.https://www.aimspress.com/article/doi/10.3934/microbiol.2022031?viewType=HTMLdefluoridationfluoride resistancebacillus sp.sodium fluoride16s rdna |
| spellingShingle | M Thirumala E Sai Krishna P Sindhu Priya S Vishnuvardhan Reddy Characterization of a novel Fluoride resistant bacterial isolate and its capability of Fluoride bioremediation AIMS Microbiology defluoridation fluoride resistance bacillus sp. sodium fluoride 16s rdna |
| title | Characterization of a novel Fluoride resistant bacterial isolate and its capability of Fluoride bioremediation |
| title_full | Characterization of a novel Fluoride resistant bacterial isolate and its capability of Fluoride bioremediation |
| title_fullStr | Characterization of a novel Fluoride resistant bacterial isolate and its capability of Fluoride bioremediation |
| title_full_unstemmed | Characterization of a novel Fluoride resistant bacterial isolate and its capability of Fluoride bioremediation |
| title_short | Characterization of a novel Fluoride resistant bacterial isolate and its capability of Fluoride bioremediation |
| title_sort | characterization of a novel fluoride resistant bacterial isolate and its capability of fluoride bioremediation |
| topic | defluoridation fluoride resistance bacillus sp. sodium fluoride 16s rdna |
| url | https://www.aimspress.com/article/doi/10.3934/microbiol.2022031?viewType=HTML |
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