Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma
Abstract Background Long noncoding RNA (lncRNA) Linc00337 has been implicated in lung, gastric, colorectal and esophageal squamous cell carcinoma progression via various mechanisms; however, its clinicopathological significance and role in pancreatic ductal adenocarcinoma (PDAC) progression remains...
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| Format: | Article |
| Language: | English |
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BMC
2020-10-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13046-020-01725-5 |
| _version_ | 1819091115475730432 |
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| author | Huakai Wang Shiyong Yu Huan Peng Yijun Shu Wenjie Zhang Qi Zhu Yingxia Wu Yijun Xu Jiqi Yan Honggang Xiang |
| author_facet | Huakai Wang Shiyong Yu Huan Peng Yijun Shu Wenjie Zhang Qi Zhu Yingxia Wu Yijun Xu Jiqi Yan Honggang Xiang |
| author_sort | Huakai Wang |
| collection | DOAJ |
| description | Abstract Background Long noncoding RNA (lncRNA) Linc00337 has been implicated in lung, gastric, colorectal and esophageal squamous cell carcinoma progression via various mechanisms; however, its clinicopathological significance and role in pancreatic ductal adenocarcinoma (PDAC) progression remains largely unknown. Methods Multiple approaches such as bioinformatic analysis, Transfection, quantitative real-time-PCR, Western blotting, animal studies, RNA-immunoprecipitation (RIP), RNA-pulldown and RNA-Fluorescence in situ hybridization (RNA-FISH) and were utilized to explore the role of Linc00337 in PDAC. Results Here we identified Linc00337 is an oncogenic lncRNA during PDAC progression. We found that the expression of Linc00337 is elevated in PDAC tissues and the higher Linc00337 predicts dismal prognosis. Functionally, Linc00337 promotes PDAC cell proliferation and cell cycle transition both in vitro and in vivo. Mechanistically, Linc00337 binds to E2F1 and functions as an E2F1 coactivator to trigger the targets expression during PDAC progression. Conclusion Our results demonstrate a reciprocal regulation mechanism between Linc00337 and E2F1 in PDAC progression and report the clinical value of Linc00337 for PDAC prognosis and treatment. |
| first_indexed | 2024-12-21T22:34:36Z |
| format | Article |
| id | doaj.art-5e95c6a1cf174f7a92b01e9fd5f833b9 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-21T22:34:36Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-5e95c6a1cf174f7a92b01e9fd5f833b92022-12-21T18:47:59ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662020-10-0139111410.1186/s13046-020-01725-5Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinomaHuakai Wang0Shiyong Yu1Huan Peng2Yijun Shu3Wenjie Zhang4Qi Zhu5Yingxia Wu6Yijun Xu7Jiqi Yan8Honggang Xiang9Department of General Surgery Pudong New Area People’s Hospital Pudong New AreaDepartment of General Surgery Pudong New Area People’s Hospital Pudong New AreaDepartment of General Surgery Pudong New Area People’s Hospital Pudong New AreaDepartment of General Surgery Pudong New Area People’s Hospital Pudong New AreaDepartment of General Surgery, Xinhua Hospital affiliated to Shanghai Jiaotong University School of MedicineDepartment of General Surgery Pudong New Area People’s Hospital Pudong New AreaDepartment of General Surgery Pudong New Area People’s Hospital Pudong New AreaDepartment of General Surgery Pudong New Area People’s Hospital Pudong New AreaDepartment of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of MedicineDepartment of General Surgery Pudong New Area People’s Hospital Pudong New AreaAbstract Background Long noncoding RNA (lncRNA) Linc00337 has been implicated in lung, gastric, colorectal and esophageal squamous cell carcinoma progression via various mechanisms; however, its clinicopathological significance and role in pancreatic ductal adenocarcinoma (PDAC) progression remains largely unknown. Methods Multiple approaches such as bioinformatic analysis, Transfection, quantitative real-time-PCR, Western blotting, animal studies, RNA-immunoprecipitation (RIP), RNA-pulldown and RNA-Fluorescence in situ hybridization (RNA-FISH) and were utilized to explore the role of Linc00337 in PDAC. Results Here we identified Linc00337 is an oncogenic lncRNA during PDAC progression. We found that the expression of Linc00337 is elevated in PDAC tissues and the higher Linc00337 predicts dismal prognosis. Functionally, Linc00337 promotes PDAC cell proliferation and cell cycle transition both in vitro and in vivo. Mechanistically, Linc00337 binds to E2F1 and functions as an E2F1 coactivator to trigger the targets expression during PDAC progression. Conclusion Our results demonstrate a reciprocal regulation mechanism between Linc00337 and E2F1 in PDAC progression and report the clinical value of Linc00337 for PDAC prognosis and treatment.http://link.springer.com/article/10.1186/s13046-020-01725-5PDACLong noncoding RNALinc00337E2F1Cell cycleCell proliferation |
| spellingShingle | Huakai Wang Shiyong Yu Huan Peng Yijun Shu Wenjie Zhang Qi Zhu Yingxia Wu Yijun Xu Jiqi Yan Honggang Xiang Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma Journal of Experimental & Clinical Cancer Research PDAC Long noncoding RNA Linc00337 E2F1 Cell cycle Cell proliferation |
| title | Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma |
| title_full | Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma |
| title_fullStr | Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma |
| title_full_unstemmed | Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma |
| title_short | Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma |
| title_sort | long noncoding rna linc00337 functions as an e2f1 co activator and promotes cell proliferation in pancreatic ductal adenocarcinoma |
| topic | PDAC Long noncoding RNA Linc00337 E2F1 Cell cycle Cell proliferation |
| url | http://link.springer.com/article/10.1186/s13046-020-01725-5 |
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