Effects of C/N ratio on the growth and protein accumulation of heterotrophic Chlorella in broken rice hydrolysate
Abstract Background Microalgae protein is considered as a sustainable alternative to animal protein in the future. Using waste for microalgal culture can upgrade low-value raw materials into high-value products, helping to offset the cost of microalgal protein production. In this study we explored t...
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-022-02204-z |
| _version_ | 1817986382154432512 |
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| author | Yihui Cai Ligong Zhai Xiaoman Fang Kangping Wu Yuhuan Liu Xian Cui Yunpu Wang Zhigang Yu Roger Ruan Tongying Liu Qi Zhang |
| author_facet | Yihui Cai Ligong Zhai Xiaoman Fang Kangping Wu Yuhuan Liu Xian Cui Yunpu Wang Zhigang Yu Roger Ruan Tongying Liu Qi Zhang |
| author_sort | Yihui Cai |
| collection | DOAJ |
| description | Abstract Background Microalgae protein is considered as a sustainable alternative to animal protein in the future. Using waste for microalgal culture can upgrade low-value raw materials into high-value products, helping to offset the cost of microalgal protein production. In this study we explored the feasibility of using microalgae heterotrophic fermentation to convert broken rice hydrolysate (BRH) into protein. Results The results showed that the increase of BRH supplemental ratio was beneficial to the increase of biomass production but not beneficial to the increase of intracellular protein content. To further improve protein production, the effect of C/N ratio on intracellular protein accumulation was studied. It was found that low C/N ratio was beneficial to the synthesis of glutamate in microalgae cells, which in turn promoted the anabolism of other amino acids and further the protein. When the C/N ratio was 12:1, the biomass productivity and protein content could reach a higher level, which were 0.90 g/L/day and 61.56%, respectively. The obtained Chlorella vulgaris biomass was rich in essential amino acids (41.80%), the essential amino acid index was as high as 89.07, and the lysine content could reach up to 4.05 g/100 g. Conclusions This study provides a theoretical basis and guidance for using Chlorella vulgaris as an industrial fermentation platform to convert broken rice into products with high nutritional value. |
| first_indexed | 2024-04-14T00:08:39Z |
| format | Article |
| id | doaj.art-5e962754ed0c455594cf209bf409bc1a |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-14T00:08:39Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-5e962754ed0c455594cf209bf409bc1a2022-12-22T02:23:25ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542022-10-0115111110.1186/s13068-022-02204-zEffects of C/N ratio on the growth and protein accumulation of heterotrophic Chlorella in broken rice hydrolysateYihui Cai0Ligong Zhai1Xiaoman Fang2Kangping Wu3Yuhuan Liu4Xian Cui5Yunpu Wang6Zhigang Yu7Roger Ruan8Tongying Liu9Qi Zhang10State Key Laboratory of Food Science and Technology, Nanchang UniversityCollege of Food Engineering, Anhui Science and Technology UniversityChina Coal Zhejiang Testing Technology Co, Ltd.State Key Laboratory of Food Science and Technology, Nanchang UniversityState Key Laboratory of Food Science and Technology, Nanchang UniversityState Key Laboratory of Food Science and Technology, Nanchang UniversityState Key Laboratory of Food Science and Technology, Nanchang UniversityAustralian Centre for Water and Environmental Biotechnology (Formerly AWMC), The University of QueenslandCenter for Biorefining and Department of Bioproducts and Biosystems Engineering, University of MinnesotaJiangxi Maternal and Child Health HospitalState Key Laboratory of Food Science and Technology, Nanchang UniversityAbstract Background Microalgae protein is considered as a sustainable alternative to animal protein in the future. Using waste for microalgal culture can upgrade low-value raw materials into high-value products, helping to offset the cost of microalgal protein production. In this study we explored the feasibility of using microalgae heterotrophic fermentation to convert broken rice hydrolysate (BRH) into protein. Results The results showed that the increase of BRH supplemental ratio was beneficial to the increase of biomass production but not beneficial to the increase of intracellular protein content. To further improve protein production, the effect of C/N ratio on intracellular protein accumulation was studied. It was found that low C/N ratio was beneficial to the synthesis of glutamate in microalgae cells, which in turn promoted the anabolism of other amino acids and further the protein. When the C/N ratio was 12:1, the biomass productivity and protein content could reach a higher level, which were 0.90 g/L/day and 61.56%, respectively. The obtained Chlorella vulgaris biomass was rich in essential amino acids (41.80%), the essential amino acid index was as high as 89.07, and the lysine content could reach up to 4.05 g/100 g. Conclusions This study provides a theoretical basis and guidance for using Chlorella vulgaris as an industrial fermentation platform to convert broken rice into products with high nutritional value.https://doi.org/10.1186/s13068-022-02204-zHeterotrophicChlorella vulgarisProteinBroken rice hydrolysateAmino acid |
| spellingShingle | Yihui Cai Ligong Zhai Xiaoman Fang Kangping Wu Yuhuan Liu Xian Cui Yunpu Wang Zhigang Yu Roger Ruan Tongying Liu Qi Zhang Effects of C/N ratio on the growth and protein accumulation of heterotrophic Chlorella in broken rice hydrolysate Biotechnology for Biofuels and Bioproducts Heterotrophic Chlorella vulgaris Protein Broken rice hydrolysate Amino acid |
| title | Effects of C/N ratio on the growth and protein accumulation of heterotrophic Chlorella in broken rice hydrolysate |
| title_full | Effects of C/N ratio on the growth and protein accumulation of heterotrophic Chlorella in broken rice hydrolysate |
| title_fullStr | Effects of C/N ratio on the growth and protein accumulation of heterotrophic Chlorella in broken rice hydrolysate |
| title_full_unstemmed | Effects of C/N ratio on the growth and protein accumulation of heterotrophic Chlorella in broken rice hydrolysate |
| title_short | Effects of C/N ratio on the growth and protein accumulation of heterotrophic Chlorella in broken rice hydrolysate |
| title_sort | effects of c n ratio on the growth and protein accumulation of heterotrophic chlorella in broken rice hydrolysate |
| topic | Heterotrophic Chlorella vulgaris Protein Broken rice hydrolysate Amino acid |
| url | https://doi.org/10.1186/s13068-022-02204-z |
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