Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance
As advances in genome engineering inch the technology towards wider clinical use—slowed by technical and ethical hurdles—a newer offshoot, termed “epigenome engineering”, offers the ability to correct disease-causing changes in the DNA without changing its sequence and, thus, without some of the unf...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/5/1238 |
| _version_ | 1827741970520342528 |
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| author | Daniel M. Sapozhnikov Moshe Szyf |
| author_facet | Daniel M. Sapozhnikov Moshe Szyf |
| author_sort | Daniel M. Sapozhnikov |
| collection | DOAJ |
| description | As advances in genome engineering inch the technology towards wider clinical use—slowed by technical and ethical hurdles—a newer offshoot, termed “epigenome engineering”, offers the ability to correct disease-causing changes in the DNA without changing its sequence and, thus, without some of the unfavorable correlates of doing so. In this review, we note some of the shortcomings of epigenetic editing technology—specifically the risks involved in the introduction of epigenetic enzymes—and highlight an alternative epigenetic editing strategy using physical occlusion to modify epigenetic marks at target sites without a requirement for any epigenetic enzyme. This may prove to be a safer alternative for more specific epigenetic editing. |
| first_indexed | 2024-03-11T03:55:40Z |
| format | Article |
| id | doaj.art-5e9d4a9712a240ec96162e4833ef4cdc |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T03:55:40Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-5e9d4a9712a240ec96162e4833ef4cdc2023-11-18T00:34:03ZengMDPI AGBiomedicines2227-90592023-04-01115123810.3390/biomedicines11051238Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric HindranceDaniel M. Sapozhnikov0Moshe Szyf1Department of Pharmacology and Therapeutics, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC H3G 1Y6, CanadaDepartment of Pharmacology and Therapeutics, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC H3G 1Y6, CanadaAs advances in genome engineering inch the technology towards wider clinical use—slowed by technical and ethical hurdles—a newer offshoot, termed “epigenome engineering”, offers the ability to correct disease-causing changes in the DNA without changing its sequence and, thus, without some of the unfavorable correlates of doing so. In this review, we note some of the shortcomings of epigenetic editing technology—specifically the risks involved in the introduction of epigenetic enzymes—and highlight an alternative epigenetic editing strategy using physical occlusion to modify epigenetic marks at target sites without a requirement for any epigenetic enzyme. This may prove to be a safer alternative for more specific epigenetic editing.https://www.mdpi.com/2227-9059/11/5/1238epigenetic editingDNA methylationCRISPR/Cas9dCas9DNMT1DNMT3A |
| spellingShingle | Daniel M. Sapozhnikov Moshe Szyf Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance Biomedicines epigenetic editing DNA methylation CRISPR/Cas9 dCas9 DNMT1 DNMT3A |
| title | Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance |
| title_full | Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance |
| title_fullStr | Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance |
| title_full_unstemmed | Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance |
| title_short | Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance |
| title_sort | increasing specificity of targeted dna methylation editing by non enzymatic crispr dcas9 based steric hindrance |
| topic | epigenetic editing DNA methylation CRISPR/Cas9 dCas9 DNMT1 DNMT3A |
| url | https://www.mdpi.com/2227-9059/11/5/1238 |
| work_keys_str_mv | AT danielmsapozhnikov increasingspecificityoftargeteddnamethylationeditingbynonenzymaticcrisprdcas9basedsterichindrance AT mosheszyf increasingspecificityoftargeteddnamethylationeditingbynonenzymaticcrisprdcas9basedsterichindrance |