Multi-Enzymatic Synthesis of Lactobionic Acid Using Wood-Degrading Enzymes Produced by White Rot Fungi
Enzymes produced by white rot fungi are involved in the synthesis of secondary metabolites with valuable biotechnological properties. One of these metabolites is lactobionic acid (LBA). The aim of this study was to characterize a novel enzyme system consisting of a cellobiose dehydrogenase from <...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/13/4/469 |
| _version_ | 1797604376456462336 |
|---|---|
| author | Wiktoria Piątek-Gołda Justyna Sulej Marcin Grąz Piotr Waśko Ewa Janik-Zabrotowicz Monika Osińska-Jaroszuk |
| author_facet | Wiktoria Piątek-Gołda Justyna Sulej Marcin Grąz Piotr Waśko Ewa Janik-Zabrotowicz Monika Osińska-Jaroszuk |
| author_sort | Wiktoria Piątek-Gołda |
| collection | DOAJ |
| description | Enzymes produced by white rot fungi are involved in the synthesis of secondary metabolites with valuable biotechnological properties. One of these metabolites is lactobionic acid (LBA). The aim of this study was to characterize a novel enzyme system consisting of a cellobiose dehydrogenase from <i>Phlebia lindtneri</i> (PlCDH), a laccase from <i>Cerrena unicolor</i> (CuLAC), a redox mediator (ABTS or DCPIP), and lactose as a substrate. We used quantitative (HPLC) and qualitative methods (TLC, FTIR) to characterise the obtained LBA. The free radical scavenging effect of the synthesised LBA was assessed with the DPPH method. Bactericidal properties were tested against Gram-negative and Gram-positive bacteria. We obtained LBA in all the systems tested; however, the study showed that the temperature of 50 °C with the addition of ABTS was the most advantageous condition for the synthesis of lactobionic acid. A mixture with 13 mM LBA synthesised at 50 °C with DCPIP showed the best antioxidant properties (40% higher compared with the commercial reagent). Furthermore, LBA had an inhibitory effect on all the bacteria tested, but the effect was better against Gram-negative bacteria with growth inhibition no lower than 70%. Summarizing the obtained data, lactobionic acid derived in a multienzymatic system is a compound with great biotechnological potential. |
| first_indexed | 2024-03-11T04:45:38Z |
| format | Article |
| id | doaj.art-5e9d7c870e3c4c9c8698e6e8b076ffac |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T04:45:38Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-5e9d7c870e3c4c9c8698e6e8b076ffac2023-11-17T20:23:55ZengMDPI AGMetabolites2218-19892023-03-0113446910.3390/metabo13040469Multi-Enzymatic Synthesis of Lactobionic Acid Using Wood-Degrading Enzymes Produced by White Rot FungiWiktoria Piątek-Gołda0Justyna Sulej1Marcin Grąz2Piotr Waśko3Ewa Janik-Zabrotowicz4Monika Osińska-Jaroszuk5Department of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska University, 19 Akademicka St., 20-033 Lublin, PolandDepartment of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska University, 19 Akademicka St., 20-033 Lublin, PolandDepartment of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska University, 19 Akademicka St., 20-033 Lublin, PolandDepartment of Plant Physiology and Biophysics, Institute of Biological Sciences, Maria Curie-Sklodowska University, 19 Akademicka St., 20-033 Lublin, PolandCore Facility of Biospectroscopy, Institute of Biological Sciences, Maria Curie-Sklodowska University, 19 Akademicka St., 20-033 Lublin, PolandDepartment of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Sklodowska University, 19 Akademicka St., 20-033 Lublin, PolandEnzymes produced by white rot fungi are involved in the synthesis of secondary metabolites with valuable biotechnological properties. One of these metabolites is lactobionic acid (LBA). The aim of this study was to characterize a novel enzyme system consisting of a cellobiose dehydrogenase from <i>Phlebia lindtneri</i> (PlCDH), a laccase from <i>Cerrena unicolor</i> (CuLAC), a redox mediator (ABTS or DCPIP), and lactose as a substrate. We used quantitative (HPLC) and qualitative methods (TLC, FTIR) to characterise the obtained LBA. The free radical scavenging effect of the synthesised LBA was assessed with the DPPH method. Bactericidal properties were tested against Gram-negative and Gram-positive bacteria. We obtained LBA in all the systems tested; however, the study showed that the temperature of 50 °C with the addition of ABTS was the most advantageous condition for the synthesis of lactobionic acid. A mixture with 13 mM LBA synthesised at 50 °C with DCPIP showed the best antioxidant properties (40% higher compared with the commercial reagent). Furthermore, LBA had an inhibitory effect on all the bacteria tested, but the effect was better against Gram-negative bacteria with growth inhibition no lower than 70%. Summarizing the obtained data, lactobionic acid derived in a multienzymatic system is a compound with great biotechnological potential.https://www.mdpi.com/2218-1989/13/4/469oxidoreductive enzymeslactobionic acidenzymatic oxidationsecondary metabolite |
| spellingShingle | Wiktoria Piątek-Gołda Justyna Sulej Marcin Grąz Piotr Waśko Ewa Janik-Zabrotowicz Monika Osińska-Jaroszuk Multi-Enzymatic Synthesis of Lactobionic Acid Using Wood-Degrading Enzymes Produced by White Rot Fungi Metabolites oxidoreductive enzymes lactobionic acid enzymatic oxidation secondary metabolite |
| title | Multi-Enzymatic Synthesis of Lactobionic Acid Using Wood-Degrading Enzymes Produced by White Rot Fungi |
| title_full | Multi-Enzymatic Synthesis of Lactobionic Acid Using Wood-Degrading Enzymes Produced by White Rot Fungi |
| title_fullStr | Multi-Enzymatic Synthesis of Lactobionic Acid Using Wood-Degrading Enzymes Produced by White Rot Fungi |
| title_full_unstemmed | Multi-Enzymatic Synthesis of Lactobionic Acid Using Wood-Degrading Enzymes Produced by White Rot Fungi |
| title_short | Multi-Enzymatic Synthesis of Lactobionic Acid Using Wood-Degrading Enzymes Produced by White Rot Fungi |
| title_sort | multi enzymatic synthesis of lactobionic acid using wood degrading enzymes produced by white rot fungi |
| topic | oxidoreductive enzymes lactobionic acid enzymatic oxidation secondary metabolite |
| url | https://www.mdpi.com/2218-1989/13/4/469 |
| work_keys_str_mv | AT wiktoriapiatekgołda multienzymaticsynthesisoflactobionicacidusingwooddegradingenzymesproducedbywhiterotfungi AT justynasulej multienzymaticsynthesisoflactobionicacidusingwooddegradingenzymesproducedbywhiterotfungi AT marcingraz multienzymaticsynthesisoflactobionicacidusingwooddegradingenzymesproducedbywhiterotfungi AT piotrwasko multienzymaticsynthesisoflactobionicacidusingwooddegradingenzymesproducedbywhiterotfungi AT ewajanikzabrotowicz multienzymaticsynthesisoflactobionicacidusingwooddegradingenzymesproducedbywhiterotfungi AT monikaosinskajaroszuk multienzymaticsynthesisoflactobionicacidusingwooddegradingenzymesproducedbywhiterotfungi |