Evaluation of Chitosan /Organoclay Composite as Bone Tissue Engineering Scaffold
I n this study, a scaffold composed of chitosan C and organoclay OC was prepared by utilizing freeze-drying method. The composition of resulting composite scaffold was evaluated using FTIR and SEM. The in vivo cytocompatibility of the composite was evaluated in vivo, by seeding MC3T3-E1 cells on...
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| Format: | Article |
| Language: | English |
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Hitit University
2017-06-01
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| Series: | Hittite Journal of Science and Engineering |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1506835 |
| _version_ | 1797662729327083520 |
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| author | Aysel Koc Demir |
| author_facet | Aysel Koc Demir |
| author_sort | Aysel Koc Demir |
| collection | DOAJ |
| description | I n this study, a scaffold composed of chitosan C and organoclay OC was prepared by utilizing freeze-drying method. The composition of resulting composite scaffold was evaluated using FTIR and SEM. The in vivo cytocompatibility of the composite was evaluated in vivo, by seeding MC3T3-E1 cells on composite scaffolds. In-vitro cell viability and proliferation were investigated using the MTT assay, while cell-scaffold constructs were evaluated using scanning electron microscopy. Preliminary in vivo experiments were also performed to evaluate tissue compatibility and ectopic boneforming potential of C/OC composite scaffolds with MC3T3-E1 cells. The findings suggest that C/OC construct supported the proliferation of MC3T3-E1 cells, presented histobiocompatibility and promoted formation of calcified matrix. Based on the results, we conclude that the characterized C/OC composite system may have potential for use in bone tissue engineering applications |
| first_indexed | 2024-03-11T19:03:18Z |
| format | Article |
| id | doaj.art-5e9f5d2f3bbf4f2386964a3dd6bcf981 |
| institution | Directory Open Access Journal |
| issn | 2148-4171 |
| language | English |
| last_indexed | 2024-03-11T19:03:18Z |
| publishDate | 2017-06-01 |
| publisher | Hitit University |
| record_format | Article |
| series | Hittite Journal of Science and Engineering |
| spelling | doaj.art-5e9f5d2f3bbf4f2386964a3dd6bcf9812023-10-10T11:17:26ZengHitit UniversityHittite Journal of Science and Engineering2148-41712017-06-0141454910.17350/HJSE19030000047150Evaluation of Chitosan /Organoclay Composite as Bone Tissue Engineering ScaffoldAysel Koc Demir0Ankara University, Department of Biochemistry, Ankara, TURKEYI n this study, a scaffold composed of chitosan C and organoclay OC was prepared by utilizing freeze-drying method. The composition of resulting composite scaffold was evaluated using FTIR and SEM. The in vivo cytocompatibility of the composite was evaluated in vivo, by seeding MC3T3-E1 cells on composite scaffolds. In-vitro cell viability and proliferation were investigated using the MTT assay, while cell-scaffold constructs were evaluated using scanning electron microscopy. Preliminary in vivo experiments were also performed to evaluate tissue compatibility and ectopic boneforming potential of C/OC composite scaffolds with MC3T3-E1 cells. The findings suggest that C/OC construct supported the proliferation of MC3T3-E1 cells, presented histobiocompatibility and promoted formation of calcified matrix. Based on the results, we conclude that the characterized C/OC composite system may have potential for use in bone tissue engineering applicationshttps://dergipark.org.tr/tr/download/article-file/1506835bone tissue engineeringchitosanorganoclaycomposite scaffoldorganically modified calcium-smectite |
| spellingShingle | Aysel Koc Demir Evaluation of Chitosan /Organoclay Composite as Bone Tissue Engineering Scaffold Hittite Journal of Science and Engineering bone tissue engineering chitosan organoclay composite scaffold organically modified calcium-smectite |
| title | Evaluation of Chitosan /Organoclay Composite as Bone Tissue Engineering Scaffold |
| title_full | Evaluation of Chitosan /Organoclay Composite as Bone Tissue Engineering Scaffold |
| title_fullStr | Evaluation of Chitosan /Organoclay Composite as Bone Tissue Engineering Scaffold |
| title_full_unstemmed | Evaluation of Chitosan /Organoclay Composite as Bone Tissue Engineering Scaffold |
| title_short | Evaluation of Chitosan /Organoclay Composite as Bone Tissue Engineering Scaffold |
| title_sort | evaluation of chitosan organoclay composite as bone tissue engineering scaffold |
| topic | bone tissue engineering chitosan organoclay composite scaffold organically modified calcium-smectite |
| url | https://dergipark.org.tr/tr/download/article-file/1506835 |
| work_keys_str_mv | AT ayselkocdemir evaluationofchitosanorganoclaycompositeasbonetissueengineeringscaffold |