Oral supplementation of Ilex paraguariensis extract and eggshell membrane exhibit beneficial effects on the skin of mature male rats
Context: Ilex paraguariensis (IP), a South American plant mostly consumed as an infusion, contains several phytocompounds with demonstrated positive effects on skin health and photoprotective properties. Eggshell membrane (ESM), a by-product of the poultry industry, also presents photoprotective pro...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2023-03-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol11/jppres22.1509_11.2.216.pdf |
| _version_ | 1797833191760855040 |
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| author | Maria R. Ramirez Ignacio Rintoul Manuel Leiva José S. Molli Juan C. Yori |
| author_facet | Maria R. Ramirez Ignacio Rintoul Manuel Leiva José S. Molli Juan C. Yori |
| author_sort | Maria R. Ramirez |
| collection | DOAJ |
| description | Context: Ilex paraguariensis (IP), a South American plant mostly consumed as an infusion, contains several phytocompounds with demonstrated positive effects on skin health and photoprotective properties. Eggshell membrane (ESM), a by-product of the poultry industry, also presents photoprotective properties. Both IP and ESM contain compounds that absorb ultraviolet radiation (UVR) and stop the mechanisms by which UVR damages the skin. The synergistic effects of these natural products on the reparation of skin structure and dermal collagen production deserves to be investigated.
Aims: To determine to what extent the oral supplementation of IP and ESM, administrated individually or in combination, promotes beneficial effects on the skin of mature Wistar rats.
Methods: The chemical composition of IP and ESM and the spectrophotometric solar protection factor (SPF) of the IP extract were characterized. In vitro, antioxidant and anti-inflammatory effects were assessed using erythrocytes. In vivo investigation consisted of macro and microscopic image analysis and diagnosis of the skin of the dorsal region of rats.
Results: The IP extract showed low SPF capacity combined with a protective effect against AAPH-induced erythrocyte haemolysis. IP extract inhibited erythrocyte haemolysis induced by both hypotonic solution and heat. The antioxidant and anti-inflammatory activity of the IP could be explained in terms of its membrane-stabilizing properties. Histologic analysis showed an increase in the collagen content of the skin of supplemented rats. A clear synergistic increase in collagen levels resulted when IP and ESM were administered together.
Conclusions: IP and ESM are beneficial for skin health, present synergistic effects, and could be used as ingredients in oral cosmetics. |
| first_indexed | 2024-04-09T14:19:28Z |
| format | Article |
| id | doaj.art-5ea0fdda43aa43888ea8d1c715bc5cba |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-04-09T14:19:28Z |
| publishDate | 2023-03-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-5ea0fdda43aa43888ea8d1c715bc5cba2023-05-05T01:25:46ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502023-03-0111221622810.56499/jppres22.1509_11.2.216Oral supplementation of Ilex paraguariensis extract and eggshell membrane exhibit beneficial effects on the skin of mature male ratsMaria R. Ramirez0https://orcid.org/0000-0001-9989-7978Ignacio Rintoul1Manuel Leiva2José S. Molli3Juan C. Yori4Instituto Universitario de Ciencias de la Salud, Fundación Barceló, Centeno 710, Santo Tome CP 3340, Argentina. Consejo Nacional de Investigaciones Científicas Técnicas (CONICET), Buenos Aires C1033AAJ, Argentina.Consejo Nacional de Investigaciones Científicas Técnicas (CONICET), Buenos Aires C1033AAJ, Argentina. Facultad deIngeniería Química. Universidad Nacional del Litoral, Colectora Ruta Nac, 168, Santa Fe CP 3000, Argentina.Instituto Universitario de Ciencias de la Salud, Fundación Barceló, Centeno 710, Santo Tome CP 3340, Argentina. Consejo Nacional de Investigaciones Científicas Técnicas (CONICET), Buenos Aires C1033AAJ, Argentina. Facultad deIngeniería Química. Universidad Nacional del Litoral, Colectora Ruta Nac, 168, Santa Fe CP 3000, Argentina.Consejo Nacional de Investigaciones Científicas Técnicas (CONICET), Buenos Aires C1033AAJ, Argentina. Facultad deIngeniería Química. Universidad Nacional del Litoral, Colectora Ruta Nac, 168, Santa Fe CP 3000, Argentina.Context: Ilex paraguariensis (IP), a South American plant mostly consumed as an infusion, contains several phytocompounds with demonstrated positive effects on skin health and photoprotective properties. Eggshell membrane (ESM), a by-product of the poultry industry, also presents photoprotective properties. Both IP and ESM contain compounds that absorb ultraviolet radiation (UVR) and stop the mechanisms by which UVR damages the skin. The synergistic effects of these natural products on the reparation of skin structure and dermal collagen production deserves to be investigated. Aims: To determine to what extent the oral supplementation of IP and ESM, administrated individually or in combination, promotes beneficial effects on the skin of mature Wistar rats. Methods: The chemical composition of IP and ESM and the spectrophotometric solar protection factor (SPF) of the IP extract were characterized. In vitro, antioxidant and anti-inflammatory effects were assessed using erythrocytes. In vivo investigation consisted of macro and microscopic image analysis and diagnosis of the skin of the dorsal region of rats. Results: The IP extract showed low SPF capacity combined with a protective effect against AAPH-induced erythrocyte haemolysis. IP extract inhibited erythrocyte haemolysis induced by both hypotonic solution and heat. The antioxidant and anti-inflammatory activity of the IP could be explained in terms of its membrane-stabilizing properties. Histologic analysis showed an increase in the collagen content of the skin of supplemented rats. A clear synergistic increase in collagen levels resulted when IP and ESM were administered together. Conclusions: IP and ESM are beneficial for skin health, present synergistic effects, and could be used as ingredients in oral cosmetics.https://jppres.com/jppres/pdf/vol11/jppres22.1509_11.2.216.pdfchemopreventive capacitymembrane-stabilizing propertyskin structure |
| spellingShingle | Maria R. Ramirez Ignacio Rintoul Manuel Leiva José S. Molli Juan C. Yori Oral supplementation of Ilex paraguariensis extract and eggshell membrane exhibit beneficial effects on the skin of mature male rats Journal of Pharmacy & Pharmacognosy Research chemopreventive capacity membrane-stabilizing property skin structure |
| title | Oral supplementation of Ilex paraguariensis extract and eggshell membrane exhibit beneficial effects on the skin of mature male rats |
| title_full | Oral supplementation of Ilex paraguariensis extract and eggshell membrane exhibit beneficial effects on the skin of mature male rats |
| title_fullStr | Oral supplementation of Ilex paraguariensis extract and eggshell membrane exhibit beneficial effects on the skin of mature male rats |
| title_full_unstemmed | Oral supplementation of Ilex paraguariensis extract and eggshell membrane exhibit beneficial effects on the skin of mature male rats |
| title_short | Oral supplementation of Ilex paraguariensis extract and eggshell membrane exhibit beneficial effects on the skin of mature male rats |
| title_sort | oral supplementation of ilex paraguariensis extract and eggshell membrane exhibit beneficial effects on the skin of mature male rats |
| topic | chemopreventive capacity membrane-stabilizing property skin structure |
| url | https://jppres.com/jppres/pdf/vol11/jppres22.1509_11.2.216.pdf |
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