Dietary Cysteamine Supplementation Remarkably Increased Feed Efficiency and Shifted Rumen Fermentation toward Glucogenic Propionate Production via Enrichment of <i>Prevotella</i> in Feedlot Lambs
Cysteamine (CS) is an essential nutritional regulator that improves the productive performance of animals by regulating somatotropic hormone secretion. To investigate the fattening potential and effects of CS on rumen microbial fermentation, 48 feedlot lambs were randomly assigned to four groups and...
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MDPI AG
2022-05-01
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| Series: | Microorganisms |
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| author | Qi-Chao Wu Wei-Kang Wang Fan Zhang Wen-Juan Li Yan-Lu Wang Liang-Kang Lv Hong-Jian Yang |
| author_facet | Qi-Chao Wu Wei-Kang Wang Fan Zhang Wen-Juan Li Yan-Lu Wang Liang-Kang Lv Hong-Jian Yang |
| author_sort | Qi-Chao Wu |
| collection | DOAJ |
| description | Cysteamine (CS) is an essential nutritional regulator that improves the productive performance of animals by regulating somatotropic hormone secretion. To investigate the fattening potential and effects of CS on rumen microbial fermentation, 48 feedlot lambs were randomly assigned to four groups and fed diets supplemented with different CS concentrations (0, 20, 40, and 60 mg/kg BW). An increase in dietary CS concentrations linearly increased the average daily gain (ADG) and dry matter intake (<i>p</i> < 0.05) but decreased the feed-to-gain ratio (<i>p</i> < 0.01). For the serum hormone, increasing the dietary CS concentration linearly decreased somatostatin and leptin concentration (<i>p</i> < 0.01) but linearly increased the concentration of growth hormone and insulin-like growth factor 1 (<i>p</i> < 0.01). Regarding rumen fermentation, ruminal pH, ammonia-N, and butyrate content did not differ among the four treatments, although dietary CS supplementation linearly increased microbial protein and propionate and decreased the amount of acetate (<i>p</i> < 0.05). Furthermore, an increase in dietary CS concentrations quadratically decreased the estimated methane production and methane production per kg ADG (<i>p</i> < 0.05). High-throughput sequencing revealed that increased dietary CS concentrations quadratically increased <i>Prevotella</i> (<i>p</i> < 0.05), and <i>Prevotella</i> and <i>norank_f__norank_o__Clostridia_UCG-014</i> were positively correlated with growth performance and rumen fermentation in a Spearman correlation analysis (r > 0.55, <i>p</i> < 0.05). Overall, a CS concentration higher than 20 mg/kg BW produced growth-promoting effects by inhibiting somatostatin concentrations and shifting the rumen toward glucogenic propionate fermentation by enriching <i>Prevotella</i>. In addition, <i>Prevotella</i> and <i>norank_f__norank_o__Clostridia_UCG-014</i> were positively correlated with growth performance in lambs. |
| first_indexed | 2024-03-09T22:59:59Z |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T22:59:59Z |
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| spelling | doaj.art-5ea2cd358dc34ca39ab47c0ce8ac22a62023-11-23T18:03:04ZengMDPI AGMicroorganisms2076-26072022-05-01106110510.3390/microorganisms10061105Dietary Cysteamine Supplementation Remarkably Increased Feed Efficiency and Shifted Rumen Fermentation toward Glucogenic Propionate Production via Enrichment of <i>Prevotella</i> in Feedlot LambsQi-Chao Wu0Wei-Kang Wang1Fan Zhang2Wen-Juan Li3Yan-Lu Wang4Liang-Kang Lv5Hong-Jian Yang6State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaCysteamine (CS) is an essential nutritional regulator that improves the productive performance of animals by regulating somatotropic hormone secretion. To investigate the fattening potential and effects of CS on rumen microbial fermentation, 48 feedlot lambs were randomly assigned to four groups and fed diets supplemented with different CS concentrations (0, 20, 40, and 60 mg/kg BW). An increase in dietary CS concentrations linearly increased the average daily gain (ADG) and dry matter intake (<i>p</i> < 0.05) but decreased the feed-to-gain ratio (<i>p</i> < 0.01). For the serum hormone, increasing the dietary CS concentration linearly decreased somatostatin and leptin concentration (<i>p</i> < 0.01) but linearly increased the concentration of growth hormone and insulin-like growth factor 1 (<i>p</i> < 0.01). Regarding rumen fermentation, ruminal pH, ammonia-N, and butyrate content did not differ among the four treatments, although dietary CS supplementation linearly increased microbial protein and propionate and decreased the amount of acetate (<i>p</i> < 0.05). Furthermore, an increase in dietary CS concentrations quadratically decreased the estimated methane production and methane production per kg ADG (<i>p</i> < 0.05). High-throughput sequencing revealed that increased dietary CS concentrations quadratically increased <i>Prevotella</i> (<i>p</i> < 0.05), and <i>Prevotella</i> and <i>norank_f__norank_o__Clostridia_UCG-014</i> were positively correlated with growth performance and rumen fermentation in a Spearman correlation analysis (r > 0.55, <i>p</i> < 0.05). Overall, a CS concentration higher than 20 mg/kg BW produced growth-promoting effects by inhibiting somatostatin concentrations and shifting the rumen toward glucogenic propionate fermentation by enriching <i>Prevotella</i>. In addition, <i>Prevotella</i> and <i>norank_f__norank_o__Clostridia_UCG-014</i> were positively correlated with growth performance in lambs.https://www.mdpi.com/2076-2607/10/6/1105cysteaminefeedlot lambrumen microorganismblood metabolitesfermentation |
| spellingShingle | Qi-Chao Wu Wei-Kang Wang Fan Zhang Wen-Juan Li Yan-Lu Wang Liang-Kang Lv Hong-Jian Yang Dietary Cysteamine Supplementation Remarkably Increased Feed Efficiency and Shifted Rumen Fermentation toward Glucogenic Propionate Production via Enrichment of <i>Prevotella</i> in Feedlot Lambs Microorganisms cysteamine feedlot lamb rumen microorganism blood metabolites fermentation |
| title | Dietary Cysteamine Supplementation Remarkably Increased Feed Efficiency and Shifted Rumen Fermentation toward Glucogenic Propionate Production via Enrichment of <i>Prevotella</i> in Feedlot Lambs |
| title_full | Dietary Cysteamine Supplementation Remarkably Increased Feed Efficiency and Shifted Rumen Fermentation toward Glucogenic Propionate Production via Enrichment of <i>Prevotella</i> in Feedlot Lambs |
| title_fullStr | Dietary Cysteamine Supplementation Remarkably Increased Feed Efficiency and Shifted Rumen Fermentation toward Glucogenic Propionate Production via Enrichment of <i>Prevotella</i> in Feedlot Lambs |
| title_full_unstemmed | Dietary Cysteamine Supplementation Remarkably Increased Feed Efficiency and Shifted Rumen Fermentation toward Glucogenic Propionate Production via Enrichment of <i>Prevotella</i> in Feedlot Lambs |
| title_short | Dietary Cysteamine Supplementation Remarkably Increased Feed Efficiency and Shifted Rumen Fermentation toward Glucogenic Propionate Production via Enrichment of <i>Prevotella</i> in Feedlot Lambs |
| title_sort | dietary cysteamine supplementation remarkably increased feed efficiency and shifted rumen fermentation toward glucogenic propionate production via enrichment of i prevotella i in feedlot lambs |
| topic | cysteamine feedlot lamb rumen microorganism blood metabolites fermentation |
| url | https://www.mdpi.com/2076-2607/10/6/1105 |
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