Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System
Manipulating transfer RNAs (tRNAs) for emancipating sense codons to simplify genetic codons in a cell-free protein synthesis (CFPS) system can offer more flexibility and controllability. Here, we provide an overview of the tRNA complement protein synthesis system construction in the tRNA-depleted Pr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.891808/full |
| _version_ | 1811247756429230080 |
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| author | Jiaojiao Li Jiaojiao Li Mengtong Tang Mengtong Tang Hao Qi Hao Qi |
| author_facet | Jiaojiao Li Jiaojiao Li Mengtong Tang Mengtong Tang Hao Qi Hao Qi |
| author_sort | Jiaojiao Li |
| collection | DOAJ |
| description | Manipulating transfer RNAs (tRNAs) for emancipating sense codons to simplify genetic codons in a cell-free protein synthesis (CFPS) system can offer more flexibility and controllability. Here, we provide an overview of the tRNA complement protein synthesis system construction in the tRNA-depleted Protein synthesis Using purified Recombinant Elements (PURE) system or S30 extract. These designed polypeptide coding sequences reduce the genetic codon and contain only a single tRNA corresponding to a single amino acid in this presented system. Strategies for removing tRNAs from cell lysates and synthesizing tRNAs in vivo/vitro are summarized and discussed in detail. Furthermore, we point out the trend toward a minimized genetic codon for reducing codon redundancy by manipulating tRNAs in the different proteins. It is hoped that the tRNA complement protein synthesis system can facilitate the construction of minimal cells and expand the biomedical application scope of synthetic biology. |
| first_indexed | 2024-04-12T15:15:15Z |
| format | Article |
| id | doaj.art-5ea37e43ec9f431b86ee2263eb897cb2 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-12T15:15:15Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-5ea37e43ec9f431b86ee2263eb897cb22022-12-22T03:27:39ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-05-011010.3389/fbioe.2022.891808891808Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free SystemJiaojiao Li0Jiaojiao Li1Mengtong Tang2Mengtong Tang3Hao Qi4Hao Qi5School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaFrontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaFrontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaFrontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin, ChinaManipulating transfer RNAs (tRNAs) for emancipating sense codons to simplify genetic codons in a cell-free protein synthesis (CFPS) system can offer more flexibility and controllability. Here, we provide an overview of the tRNA complement protein synthesis system construction in the tRNA-depleted Protein synthesis Using purified Recombinant Elements (PURE) system or S30 extract. These designed polypeptide coding sequences reduce the genetic codon and contain only a single tRNA corresponding to a single amino acid in this presented system. Strategies for removing tRNAs from cell lysates and synthesizing tRNAs in vivo/vitro are summarized and discussed in detail. Furthermore, we point out the trend toward a minimized genetic codon for reducing codon redundancy by manipulating tRNAs in the different proteins. It is hoped that the tRNA complement protein synthesis system can facilitate the construction of minimal cells and expand the biomedical application scope of synthetic biology.https://www.frontiersin.org/articles/10.3389/fbioe.2022.891808/fullcell-free systemtransfer RNAcodon-reducedin vitro-transcriptionprotein synthesis |
| spellingShingle | Jiaojiao Li Jiaojiao Li Mengtong Tang Mengtong Tang Hao Qi Hao Qi Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System Frontiers in Bioengineering and Biotechnology cell-free system transfer RNA codon-reduced in vitro-transcription protein synthesis |
| title | Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System |
| title_full | Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System |
| title_fullStr | Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System |
| title_full_unstemmed | Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System |
| title_short | Codon-Reduced Protein Synthesis With Manipulating tRNA Components in Cell-Free System |
| title_sort | codon reduced protein synthesis with manipulating trna components in cell free system |
| topic | cell-free system transfer RNA codon-reduced in vitro-transcription protein synthesis |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.891808/full |
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