Influence of the H1 Antihistamine Mepyramine on the Antibacterial Effect of Florfenicol in Pigs
The effect of florfenicol against <i>Escherichia coli</i> (<i>E. coli</i>) was investigated in vivo to confirm results of an in vitro study of Bruer et al. (2019), which has shown positive effects of various antibacterial agents in combination with the antihistamine mepyramin...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/8/9/197 |
| _version_ | 1797516992124551168 |
|---|---|
| author | Gustav G. Bruer Daria Gödecke Manfred Kietzmann Jessica Meißner |
| author_facet | Gustav G. Bruer Daria Gödecke Manfred Kietzmann Jessica Meißner |
| author_sort | Gustav G. Bruer |
| collection | DOAJ |
| description | The effect of florfenicol against <i>Escherichia coli</i> (<i>E. coli</i>) was investigated in vivo to confirm results of an in vitro study of Bruer et al. (2019), which has shown positive effects of various antibacterial agents in combination with the antihistamine mepyramine (MEP). Therefore, pigs were treated in three different settings: An untreated control group, 10 mg/kg florfenicol (FFC) and 10 mg/kg FFC in combination with 20 mg/kg MEP. <i>E. coli</i> were isolated from faecal samples and analyzed in growth quantity and resistance to FFC. The FFC medication induced an increased number of resistant <i>E. coli</i> strains isolated from faecal samples. The number of colonies detected after cultivation of animal samples treated with 10 mg/kg FFC was higher than the number of colonies after treatment with 10 mg/kg FFC in combination with of FFC and MEP. Furthermore, the effect of both compounds was examined on bacterial susceptibility of <i>Pasteurella multocida</i> in vitro, where the combination of FFC with MEP resulted in a diminished minimum inhibitory concentration. We confirmed the development of bacterial resistance in the intestine as non-target tissue caused by the use of the antibacterial agent florfenicol. Moreover, the combination of FFC with an antihistamine like MEP offers a possibility to enhance the efficacy of an antibacterial treatment and modifies the effect on gut microbiota. |
| first_indexed | 2024-03-10T07:08:39Z |
| format | Article |
| id | doaj.art-5ea66fd3b23a4298b2aa57a25c03dbc9 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-10T07:08:39Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-5ea66fd3b23a4298b2aa57a25c03dbc92023-11-22T15:36:03ZengMDPI AGVeterinary Sciences2306-73812021-09-018919710.3390/vetsci8090197Influence of the H1 Antihistamine Mepyramine on the Antibacterial Effect of Florfenicol in PigsGustav G. Bruer0Daria Gödecke1Manfred Kietzmann2Jessica Meißner3Chemical Safety and Toxicology, Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), 30625 Hannover, GermanyDepartment of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, GermanyDepartment of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, GermanyDepartment of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, GermanyThe effect of florfenicol against <i>Escherichia coli</i> (<i>E. coli</i>) was investigated in vivo to confirm results of an in vitro study of Bruer et al. (2019), which has shown positive effects of various antibacterial agents in combination with the antihistamine mepyramine (MEP). Therefore, pigs were treated in three different settings: An untreated control group, 10 mg/kg florfenicol (FFC) and 10 mg/kg FFC in combination with 20 mg/kg MEP. <i>E. coli</i> were isolated from faecal samples and analyzed in growth quantity and resistance to FFC. The FFC medication induced an increased number of resistant <i>E. coli</i> strains isolated from faecal samples. The number of colonies detected after cultivation of animal samples treated with 10 mg/kg FFC was higher than the number of colonies after treatment with 10 mg/kg FFC in combination with of FFC and MEP. Furthermore, the effect of both compounds was examined on bacterial susceptibility of <i>Pasteurella multocida</i> in vitro, where the combination of FFC with MEP resulted in a diminished minimum inhibitory concentration. We confirmed the development of bacterial resistance in the intestine as non-target tissue caused by the use of the antibacterial agent florfenicol. Moreover, the combination of FFC with an antihistamine like MEP offers a possibility to enhance the efficacy of an antibacterial treatment and modifies the effect on gut microbiota.https://www.mdpi.com/2306-7381/8/9/197<i>Escherichia coli</i>antihistamineantibacterialpig |
| spellingShingle | Gustav G. Bruer Daria Gödecke Manfred Kietzmann Jessica Meißner Influence of the H1 Antihistamine Mepyramine on the Antibacterial Effect of Florfenicol in Pigs Veterinary Sciences <i>Escherichia coli</i> antihistamine antibacterial pig |
| title | Influence of the H1 Antihistamine Mepyramine on the Antibacterial Effect of Florfenicol in Pigs |
| title_full | Influence of the H1 Antihistamine Mepyramine on the Antibacterial Effect of Florfenicol in Pigs |
| title_fullStr | Influence of the H1 Antihistamine Mepyramine on the Antibacterial Effect of Florfenicol in Pigs |
| title_full_unstemmed | Influence of the H1 Antihistamine Mepyramine on the Antibacterial Effect of Florfenicol in Pigs |
| title_short | Influence of the H1 Antihistamine Mepyramine on the Antibacterial Effect of Florfenicol in Pigs |
| title_sort | influence of the h1 antihistamine mepyramine on the antibacterial effect of florfenicol in pigs |
| topic | <i>Escherichia coli</i> antihistamine antibacterial pig |
| url | https://www.mdpi.com/2306-7381/8/9/197 |
| work_keys_str_mv | AT gustavgbruer influenceoftheh1antihistaminemepyramineontheantibacterialeffectofflorfenicolinpigs AT dariagodecke influenceoftheh1antihistaminemepyramineontheantibacterialeffectofflorfenicolinpigs AT manfredkietzmann influenceoftheh1antihistaminemepyramineontheantibacterialeffectofflorfenicolinpigs AT jessicameißner influenceoftheh1antihistaminemepyramineontheantibacterialeffectofflorfenicolinpigs |