In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from Nepal

Wild edible mushrooms are becoming endangered all over the world. Very few wild edible mushrooms are found in natural habitat. Volvariella bombycina is an edible and medicinal mushroom. The mushroom was collected in natural habitat growing on Populus tree. Mycelium of the mushroom was developed in P...

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Main Authors: Pradip Kumar Chaudhary, Mitesh Shrestha, Bal Hari Poudel, Mahesh Kumar Adhikari
Format: Article
Language:English
Published: Biotechnology Society of Nepal 2017-12-01
Series:Nepal Journal of Biotechnology
Subjects:
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author Pradip Kumar Chaudhary
Mitesh Shrestha
Bal Hari Poudel
Mahesh Kumar Adhikari
author_facet Pradip Kumar Chaudhary
Mitesh Shrestha
Bal Hari Poudel
Mahesh Kumar Adhikari
author_sort Pradip Kumar Chaudhary
collection DOAJ
description Wild edible mushrooms are becoming endangered all over the world. Very few wild edible mushrooms are found in natural habitat. Volvariella bombycina is an edible and medicinal mushroom. The mushroom was collected in natural habitat growing on Populus tree. Mycelium of the mushroom was developed in PDA slant tubes by tissue culture method, incubated at 25°C for 1-2 weeks. Spawn was developed in wheat grains after incubation at 25°C for 2-3 weeks. Substrates were formulated for the development of fruiting bodies by combination of paddy straw, saw dust and rice husk. Fruiting bodies of V. bombycina was cultivated in these substrates after incubation at 28 ± 2°C for 2-4 weeks. The work describes the optimized process for in vitro culture of wild edible mushroom Volvariella bomybycina.
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spelling doaj.art-5ea68f17d220449ab8d2fed27e5881742022-12-21T22:09:34ZengBiotechnology Society of NepalNepal Journal of Biotechnology2091-11302467-93132017-12-0151273110.3126/njb.v5i1.18867In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from NepalPradip Kumar Chaudhary0Mitesh Shrestha1Bal Hari Poudel2Mahesh Kumar Adhikari3Central Department of Biotechnology, Tribhuvan University and Nepal Academy of Science and Technology, KhumaltarCentral Department of Biotechnology, Tribhuvan University and Nepal Academy of Science and Technology, KhumaltarCentral Department of Biotechnology, Tribhuvan UniversityCentral Department of Biotechnology, Tribhuvan University and National Herbarium & Plant Laboratories, Department of Plant Resources, Ministry of Forests and Soil ConservationWild edible mushrooms are becoming endangered all over the world. Very few wild edible mushrooms are found in natural habitat. Volvariella bombycina is an edible and medicinal mushroom. The mushroom was collected in natural habitat growing on Populus tree. Mycelium of the mushroom was developed in PDA slant tubes by tissue culture method, incubated at 25°C for 1-2 weeks. Spawn was developed in wheat grains after incubation at 25°C for 2-3 weeks. Substrates were formulated for the development of fruiting bodies by combination of paddy straw, saw dust and rice husk. Fruiting bodies of V. bombycina was cultivated in these substrates after incubation at 28 ± 2°C for 2-4 weeks. The work describes the optimized process for in vitro culture of wild edible mushroom Volvariella bomybycina.mushroomwildediblecultivationvolvariella bombycina
spellingShingle Pradip Kumar Chaudhary
Mitesh Shrestha
Bal Hari Poudel
Mahesh Kumar Adhikari
In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from Nepal
Nepal Journal of Biotechnology
mushroom
wild
edible
cultivation
volvariella bombycina
title In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from Nepal
title_full In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from Nepal
title_fullStr In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from Nepal
title_full_unstemmed In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from Nepal
title_short In vitro Cultivation of Newly Reported Wild Edible Mushroom Volvariella bomybycina from Nepal
title_sort in vitro cultivation of newly reported wild edible mushroom volvariella bomybycina from nepal
topic mushroom
wild
edible
cultivation
volvariella bombycina
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AT miteshshrestha invitrocultivationofnewlyreportedwildediblemushroomvolvariellabomybycinafromnepal
AT balharipoudel invitrocultivationofnewlyreportedwildediblemushroomvolvariellabomybycinafromnepal
AT maheshkumaradhikari invitrocultivationofnewlyreportedwildediblemushroomvolvariellabomybycinafromnepal