Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas
Aim: To investigate whether the aberrant expression of microRNA (miR)-196a and miR-196b can be used as potential prognostic markers of human osteosarcoma. Methods: Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis was performed to detect the expression levels...
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MDPI AG
2014-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/15/4/6544 |
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| author | Chun Zhang Cong Yao Haopeng Li Guoyu Wang Xijng He |
| author_facet | Chun Zhang Cong Yao Haopeng Li Guoyu Wang Xijng He |
| author_sort | Chun Zhang |
| collection | DOAJ |
| description | Aim: To investigate whether the aberrant expression of microRNA (miR)-196a and miR-196b can be used as potential prognostic markers of human osteosarcoma. Methods: Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis was performed to detect the expression levels of miR-196a and miR-196b in osteosarcoma tissues and patients’ sera. Results: Expression levels of miR-196a and miR-196b in osteosarcoma tissues were both significantly higher than those in noncancerous bone tissues (both p < 0.001), in line with which, the serum levels of the two miRNAs were also markedly upregulated in patients with osteosarcomas compared with healthy controls (both p < 0.001). Then, the elevation of serum miR-196a and miR-196b levels both more frequently occurred in osteosarcoma patients with high tumor grade (p = 0.008 and 0.01, respectively), positive metastasis (p = 0.001 and 0.006, respectively) and recurrence (p = 0.001 and 0.006, respectively). Moreover, high serum miR-196a, high serum miR-196b and conjoined expression of miR-196a/miR-196b were all independent prognostic factors for OS (overall survival) and DFS (disease-free survival) of osteosarcoma patients. Conclusion: Our present data indicate the involvement of miR-196a and miR-196b upregulation in the pathogenesis of osteosarcoma. More importantly, the altered levels of circulating miR-196a and miR-196b might have great potential to serve as novel and non-invasive prognostic factors for this malignancy. |
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| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T13:33:44Z |
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| spelling | doaj.art-5ea70ad2320a4536b33474843cb0d8d32022-12-22T02:44:50ZengMDPI AGInternational Journal of Molecular Sciences1422-00672014-04-011546544655510.3390/ijms15046544ijms15046544Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with OsteosarcomasChun Zhang0Cong Yao1Haopeng Li2Guoyu Wang3Xijng He4Department of Orthopedic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, ChinaDepartment of Aesthetic Plastic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, ChinaDepartment of Orthopedic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, ChinaDepartment of Orthopedic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, ChinaDepartment of Orthopedic Surgery, the Second Hospital Affiliated to Xi'an Jiao Tong University School of Medicine, Xi'an 710004, Shaanxi, ChinaAim: To investigate whether the aberrant expression of microRNA (miR)-196a and miR-196b can be used as potential prognostic markers of human osteosarcoma. Methods: Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis was performed to detect the expression levels of miR-196a and miR-196b in osteosarcoma tissues and patients’ sera. Results: Expression levels of miR-196a and miR-196b in osteosarcoma tissues were both significantly higher than those in noncancerous bone tissues (both p < 0.001), in line with which, the serum levels of the two miRNAs were also markedly upregulated in patients with osteosarcomas compared with healthy controls (both p < 0.001). Then, the elevation of serum miR-196a and miR-196b levels both more frequently occurred in osteosarcoma patients with high tumor grade (p = 0.008 and 0.01, respectively), positive metastasis (p = 0.001 and 0.006, respectively) and recurrence (p = 0.001 and 0.006, respectively). Moreover, high serum miR-196a, high serum miR-196b and conjoined expression of miR-196a/miR-196b were all independent prognostic factors for OS (overall survival) and DFS (disease-free survival) of osteosarcoma patients. Conclusion: Our present data indicate the involvement of miR-196a and miR-196b upregulation in the pathogenesis of osteosarcoma. More importantly, the altered levels of circulating miR-196a and miR-196b might have great potential to serve as novel and non-invasive prognostic factors for this malignancy.http://www.mdpi.com/1422-0067/15/4/6544osteosarcomamicroRNA-196amicroRNA-196bserumclinicopathological featuresoverall survivaldisease-free survival |
| spellingShingle | Chun Zhang Cong Yao Haopeng Li Guoyu Wang Xijng He Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas International Journal of Molecular Sciences osteosarcoma microRNA-196a microRNA-196b serum clinicopathological features overall survival disease-free survival |
| title | Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas |
| title_full | Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas |
| title_fullStr | Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas |
| title_full_unstemmed | Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas |
| title_short | Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas |
| title_sort | combined elevation of microrna 196a and microrna 196b in sera predicts unfavorable prognosis in patients with osteosarcomas |
| topic | osteosarcoma microRNA-196a microRNA-196b serum clinicopathological features overall survival disease-free survival |
| url | http://www.mdpi.com/1422-0067/15/4/6544 |
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