In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis
The aim of the present paper was to evaluate the larvicidal effect of Enterococcus faecalis CECT7121 (Ef7121) on the Toxocara canis cycle both in vitro and in vivo. For the in vitro experiments, T. canis larvae were incubated with the supernatants of Ef7121 (EI) and mutant Ef7121 (EIm), in a pre-cul...
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Fundação Oswaldo Cruz (FIOCRUZ)
2010-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500003 |
| _version_ | 1797712671057903616 |
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| author | Paula G Chiodo Mónica D Sparo Betina C Pezzani Marta C Minvielle Juan A Basualdo |
| author_facet | Paula G Chiodo Mónica D Sparo Betina C Pezzani Marta C Minvielle Juan A Basualdo |
| author_sort | Paula G Chiodo |
| collection | DOAJ |
| description | The aim of the present paper was to evaluate the larvicidal effect of Enterococcus faecalis CECT7121 (Ef7121) on the Toxocara canis cycle both in vitro and in vivo. For the in vitro experiments, T. canis larvae were incubated with the supernatants of Ef7121 (EI) and mutant Ef7121 (EIm), in a pre-culture of Ef7121 (EII) and in a fresh culture with Ef7121 (EIII) and the Ef7121 mutant strain (EIIIm). The viability of the larvae was calculated after a 48 h incubation. A significant reduction of the viability of T. canis larvae was observed in EI, EII and EIII. A decrease of this inhibitory effect was observed in EIm and EIIIm (p = 0.008). In the in vivo experiments, mice were orally inoculated with three doses of Ef7121. To study the probiotic persistence in the intestine, the animals were sacrificed every four days and their intestines were dissected. The initial average bacterial levels were 9.7 x 10(4) for Ef7121 (colony forming units/g). At the end of the assay the levels were 1.46 x 10(4). No bacterial translocation was detected in mesenteric lymphatic nodules and spleen. Ef7121 interference with the biological cycle was evaluated in mice challenged with T. canis. The interference was significant when the mice were challenged with probiotic and T. canis simultaneously (p = 0.001), but it was not significant when the challenge was performed 15 days after administration of the bacterial inoculum (p = 0.06). In conclusion, Ef7121 possessed in vitro and in vivo larvicidal activity. |
| first_indexed | 2024-03-12T07:25:10Z |
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| id | doaj.art-5ea7b3684f5d4bfea900c70689b84785 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:25:10Z |
| publishDate | 2010-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-5ea7b3684f5d4bfea900c70689b847852023-09-02T22:10:41ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602010-08-01105561562010.1590/S0074-02762010000500003In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canisPaula G ChiodoMónica D SparoBetina C PezzaniMarta C MinvielleJuan A BasualdoThe aim of the present paper was to evaluate the larvicidal effect of Enterococcus faecalis CECT7121 (Ef7121) on the Toxocara canis cycle both in vitro and in vivo. For the in vitro experiments, T. canis larvae were incubated with the supernatants of Ef7121 (EI) and mutant Ef7121 (EIm), in a pre-culture of Ef7121 (EII) and in a fresh culture with Ef7121 (EIII) and the Ef7121 mutant strain (EIIIm). The viability of the larvae was calculated after a 48 h incubation. A significant reduction of the viability of T. canis larvae was observed in EI, EII and EIII. A decrease of this inhibitory effect was observed in EIm and EIIIm (p = 0.008). In the in vivo experiments, mice were orally inoculated with three doses of Ef7121. To study the probiotic persistence in the intestine, the animals were sacrificed every four days and their intestines were dissected. The initial average bacterial levels were 9.7 x 10(4) for Ef7121 (colony forming units/g). At the end of the assay the levels were 1.46 x 10(4). No bacterial translocation was detected in mesenteric lymphatic nodules and spleen. Ef7121 interference with the biological cycle was evaluated in mice challenged with T. canis. The interference was significant when the mice were challenged with probiotic and T. canis simultaneously (p = 0.001), but it was not significant when the challenge was performed 15 days after administration of the bacterial inoculum (p = 0.06). In conclusion, Ef7121 possessed in vitro and in vivo larvicidal activity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500003Enterococcus faecalis CECT7121Toxocara canisprobioticsbiological cycle |
| spellingShingle | Paula G Chiodo Mónica D Sparo Betina C Pezzani Marta C Minvielle Juan A Basualdo In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis Memorias do Instituto Oswaldo Cruz Enterococcus faecalis CECT7121 Toxocara canis probiotics biological cycle |
| title | In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis |
| title_full | In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis |
| title_fullStr | In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis |
| title_full_unstemmed | In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis |
| title_short | In vitro and in vivo effects of Enterococcus faecalis CECT7121 on Toxocara canis |
| title_sort | in vitro and in vivo effects of enterococcus faecalis cect7121 on toxocara canis |
| topic | Enterococcus faecalis CECT7121 Toxocara canis probiotics biological cycle |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500003 |
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