Hemoglobin Reassembly of Antimicrobial Fragments from the Midgut of <i>Triatoma infestans</i>
Hemoglobin is one of the most important molecules of the human body. Beyond its physiological activity, hemoglobins are able to inhibit the growth of several microorganisms. Since 1999, studies have reported that antimicrobial peptides can be produced by blood-feeding insects through hemoglobin dige...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/2/261 |
| _version_ | 1819024953234685952 |
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| author | Laura Cristina Lima Diniz Pedro Ismael da Silva Junior |
| author_facet | Laura Cristina Lima Diniz Pedro Ismael da Silva Junior |
| author_sort | Laura Cristina Lima Diniz |
| collection | DOAJ |
| description | Hemoglobin is one of the most important molecules of the human body. Beyond its physiological activity, hemoglobins are able to inhibit the growth of several microorganisms. Since 1999, studies have reported that antimicrobial peptides can be produced by blood-feeding insects through hemoglobin digestion, and it has been reported that <i>Triatoma infestans</i> can generate an antimicrobial fragment from human fibrinopeptide. Thus <i>T. infestans</i> intestinal content was analyzed through Reverse Phase High-Performance Liquid Chromatography (RP-HPLC), the eluted fractions were tested against <i>Micrococcus luteus</i>, <i>Escherichia coli</i> and <i>Staphylococcus aureus,</i> and the active fractions submitted to mass spectrometry. The data obtained were compared to hemoglobin databases to verify the presence of hemoglobin-derived fragments. Ten fractions eluted from chromatography presented antimicrobial activity, and when analyzed through mass spectrometry revealed the presence of 8 murine hemoglobin α-chain fragments and 24 fragments from murine hemoglobin β fragments. Through the compilation of the fragments is possible to obtain over 67% coverage of both sequences. Part of the amino acid sequences corresponds to the sequences already identified on other intestinal contents of arthropods, and are highly conserved between the blood of other wild animals that are the most common intermediate hosts of Chagas’ disease in Brazil and some of the main natural blood source for triatomines. |
| first_indexed | 2024-12-21T05:02:58Z |
| format | Article |
| id | doaj.art-5eaabfa621e844af82ac859ad629bd37 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-21T05:02:58Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-5eaabfa621e844af82ac859ad629bd372022-12-21T19:15:11ZengMDPI AGBiomolecules2218-273X2020-02-0110226110.3390/biom10020261biom10020261Hemoglobin Reassembly of Antimicrobial Fragments from the Midgut of <i>Triatoma infestans</i>Laura Cristina Lima Diniz0Pedro Ismael da Silva Junior1Laboratory of Applied Toxinology, Butantan Institute, CEP: 05503-900, São Paulo, SP, BrazilLaboratory of Applied Toxinology, Butantan Institute, CEP: 05503-900, São Paulo, SP, BrazilHemoglobin is one of the most important molecules of the human body. Beyond its physiological activity, hemoglobins are able to inhibit the growth of several microorganisms. Since 1999, studies have reported that antimicrobial peptides can be produced by blood-feeding insects through hemoglobin digestion, and it has been reported that <i>Triatoma infestans</i> can generate an antimicrobial fragment from human fibrinopeptide. Thus <i>T. infestans</i> intestinal content was analyzed through Reverse Phase High-Performance Liquid Chromatography (RP-HPLC), the eluted fractions were tested against <i>Micrococcus luteus</i>, <i>Escherichia coli</i> and <i>Staphylococcus aureus,</i> and the active fractions submitted to mass spectrometry. The data obtained were compared to hemoglobin databases to verify the presence of hemoglobin-derived fragments. Ten fractions eluted from chromatography presented antimicrobial activity, and when analyzed through mass spectrometry revealed the presence of 8 murine hemoglobin α-chain fragments and 24 fragments from murine hemoglobin β fragments. Through the compilation of the fragments is possible to obtain over 67% coverage of both sequences. Part of the amino acid sequences corresponds to the sequences already identified on other intestinal contents of arthropods, and are highly conserved between the blood of other wild animals that are the most common intermediate hosts of Chagas’ disease in Brazil and some of the main natural blood source for triatomines.https://www.mdpi.com/2218-273X/10/2/261<i>triatoma infestans</i>antimicrobial fragmentshemoglobintriatomines |
| spellingShingle | Laura Cristina Lima Diniz Pedro Ismael da Silva Junior Hemoglobin Reassembly of Antimicrobial Fragments from the Midgut of <i>Triatoma infestans</i> Biomolecules <i>triatoma infestans</i> antimicrobial fragments hemoglobin triatomines |
| title | Hemoglobin Reassembly of Antimicrobial Fragments from the Midgut of <i>Triatoma infestans</i> |
| title_full | Hemoglobin Reassembly of Antimicrobial Fragments from the Midgut of <i>Triatoma infestans</i> |
| title_fullStr | Hemoglobin Reassembly of Antimicrobial Fragments from the Midgut of <i>Triatoma infestans</i> |
| title_full_unstemmed | Hemoglobin Reassembly of Antimicrobial Fragments from the Midgut of <i>Triatoma infestans</i> |
| title_short | Hemoglobin Reassembly of Antimicrobial Fragments from the Midgut of <i>Triatoma infestans</i> |
| title_sort | hemoglobin reassembly of antimicrobial fragments from the midgut of i triatoma infestans i |
| topic | <i>triatoma infestans</i> antimicrobial fragments hemoglobin triatomines |
| url | https://www.mdpi.com/2218-273X/10/2/261 |
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