Occurrence, Genetic Diversities And Antibiotic Resistance Profiles Of Salmonella Serovars Isolated From Chickens
Stephen Abiola Akinola,1 Mulunda Mwanza,2 Collins Njie Ateba1 1Antimicrobial Resistance and Phage Bio-Control Group, Department of Microbiology, Faculty of Natural and Agricultural Sciences, North West University, Mmabatho 2745, South Africa; 2Center for Animal Health Studies, Faculty of Natural and...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-10-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/occurrence-genetic-diversities-and-antibiotic-resistance-profiles-of-s-peer-reviewed-article-IDR |
| _version_ | 1818334532347101184 |
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| author | Akinola SA Mwanza M Ateba CN |
| author_facet | Akinola SA Mwanza M Ateba CN |
| author_sort | Akinola SA |
| collection | DOAJ |
| description | Stephen Abiola Akinola,1 Mulunda Mwanza,2 Collins Njie Ateba1 1Antimicrobial Resistance and Phage Bio-Control Group, Department of Microbiology, Faculty of Natural and Agricultural Sciences, North West University, Mmabatho 2745, South Africa; 2Center for Animal Health Studies, Faculty of Natural and Agricultural Sciences, North West University, Mmabatho 2745, South AfricaCorrespondence: Collins Njie AtebaAntimicrobial Resistance and Phage Bio-control Group, Department of Microbiology, Faculty of Natural and Agricultural Sciences, North West University, Private Bag X2046, Mmabatho 2745, South AfricaTel +27 18 489 2720Email collins.ateba@nwu.ac.zaPurpose: Contamination with Salmonella on food products and poultry in particular has been linked to foodborne infections and/or death in humans. This study investigated the occurrence, genetic diversities and antibiotic resistance profiles of Salmonella strains isolated from chickens.Patients and methods: Twenty each duplicate faecal swab samples were collected from five different poultry pens of broilers, layers and indigenous chickens in the North-West Province, South Africa. Isolates identities were confirmed through amplification and sequence analysis of 16S rRNA and the invA gene fragments after which phylogenetic tree was constructed. Salmonella enteritidis (ATCC:13076TM), Salmonella Typhimurium (ATCC:14028TM) and E. coli (ATCC:259622TM) were used as positive and negative controls, respectively. The serotypes of Salmonella isolates were determined. Antibiotic-resistant profiles of the isolates against eleven antimicrobial agents were determined.Results: Eighty-four (84%) of representative isolates possessed the invA genes. The percent occurrence and diversity of Salmonella subspecies in chickens were 1.81–30.9% and was highest in Salmonella enterica subsp. enterica. Notably, the following serotypes Salmonella bongori (10.09%), Salmonella Pullorum (1.81%), Salmonella Typhimurium (12.72%), Salmonella Weltevreden, Salmonella Chingola, Salmonella Houten and Salmonella Bareily (1.81%). Isolates (96.6%) displayed multidrug resistance profiles and the identification of isolates with more than nine antibiotic resistance was a cause for concern.Conclusion: This study indicates that isolates had pre-exposure histories to the antibiotics tested and may pose severe threats to food security and public health.Keywords: Salmonella, diversity, antimicrobial resistance, phylogenetic, chickens |
| first_indexed | 2024-12-13T14:09:02Z |
| format | Article |
| id | doaj.art-5eafcf7a0a374543a63629991ca368ac |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-13T14:09:02Z |
| publishDate | 2019-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-5eafcf7a0a374543a63629991ca368ac2022-12-21T23:42:30ZengDove Medical PressInfection and Drug Resistance1178-69732019-10-01Volume 123327334249320Occurrence, Genetic Diversities And Antibiotic Resistance Profiles Of Salmonella Serovars Isolated From ChickensAkinola SAMwanza MAteba CNStephen Abiola Akinola,1 Mulunda Mwanza,2 Collins Njie Ateba1 1Antimicrobial Resistance and Phage Bio-Control Group, Department of Microbiology, Faculty of Natural and Agricultural Sciences, North West University, Mmabatho 2745, South Africa; 2Center for Animal Health Studies, Faculty of Natural and Agricultural Sciences, North West University, Mmabatho 2745, South AfricaCorrespondence: Collins Njie AtebaAntimicrobial Resistance and Phage Bio-control Group, Department of Microbiology, Faculty of Natural and Agricultural Sciences, North West University, Private Bag X2046, Mmabatho 2745, South AfricaTel +27 18 489 2720Email collins.ateba@nwu.ac.zaPurpose: Contamination with Salmonella on food products and poultry in particular has been linked to foodborne infections and/or death in humans. This study investigated the occurrence, genetic diversities and antibiotic resistance profiles of Salmonella strains isolated from chickens.Patients and methods: Twenty each duplicate faecal swab samples were collected from five different poultry pens of broilers, layers and indigenous chickens in the North-West Province, South Africa. Isolates identities were confirmed through amplification and sequence analysis of 16S rRNA and the invA gene fragments after which phylogenetic tree was constructed. Salmonella enteritidis (ATCC:13076TM), Salmonella Typhimurium (ATCC:14028TM) and E. coli (ATCC:259622TM) were used as positive and negative controls, respectively. The serotypes of Salmonella isolates were determined. Antibiotic-resistant profiles of the isolates against eleven antimicrobial agents were determined.Results: Eighty-four (84%) of representative isolates possessed the invA genes. The percent occurrence and diversity of Salmonella subspecies in chickens were 1.81–30.9% and was highest in Salmonella enterica subsp. enterica. Notably, the following serotypes Salmonella bongori (10.09%), Salmonella Pullorum (1.81%), Salmonella Typhimurium (12.72%), Salmonella Weltevreden, Salmonella Chingola, Salmonella Houten and Salmonella Bareily (1.81%). Isolates (96.6%) displayed multidrug resistance profiles and the identification of isolates with more than nine antibiotic resistance was a cause for concern.Conclusion: This study indicates that isolates had pre-exposure histories to the antibiotics tested and may pose severe threats to food security and public health.Keywords: Salmonella, diversity, antimicrobial resistance, phylogenetic, chickenshttps://www.dovepress.com/occurrence-genetic-diversities-and-antibiotic-resistance-profiles-of-s-peer-reviewed-article-IDRSalmonellaDiversityAntimicrobial resistancePhylogeneticChickens |
| spellingShingle | Akinola SA Mwanza M Ateba CN Occurrence, Genetic Diversities And Antibiotic Resistance Profiles Of Salmonella Serovars Isolated From Chickens Infection and Drug Resistance Salmonella Diversity Antimicrobial resistance Phylogenetic Chickens |
| title | Occurrence, Genetic Diversities And Antibiotic Resistance Profiles Of Salmonella Serovars Isolated From Chickens |
| title_full | Occurrence, Genetic Diversities And Antibiotic Resistance Profiles Of Salmonella Serovars Isolated From Chickens |
| title_fullStr | Occurrence, Genetic Diversities And Antibiotic Resistance Profiles Of Salmonella Serovars Isolated From Chickens |
| title_full_unstemmed | Occurrence, Genetic Diversities And Antibiotic Resistance Profiles Of Salmonella Serovars Isolated From Chickens |
| title_short | Occurrence, Genetic Diversities And Antibiotic Resistance Profiles Of Salmonella Serovars Isolated From Chickens |
| title_sort | occurrence genetic diversities and antibiotic resistance profiles of salmonella serovars isolated from chickens |
| topic | Salmonella Diversity Antimicrobial resistance Phylogenetic Chickens |
| url | https://www.dovepress.com/occurrence-genetic-diversities-and-antibiotic-resistance-profiles-of-s-peer-reviewed-article-IDR |
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