Gender and age effects on the expression of genes related to lipid metabolism in broiler's liver
Two experiments were conducted to assess gender (Experiment 1) and age (Experiment 2) effects on the expression of genes related to lipid metabolism in broiler chickens. The expression of fatty acid synthase (FAS), apolipoprotein A-I (APOA-I), apolipoprotein B (APOB), adiponectin (ADIPOQ), liver kin...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2018-03-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201803-0003_gender-and-age-effects-on-the-expression-of-genes-related-to-lipid-metabolism-in-broiler-s-liver.php |
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| author | Angélica de Souza Khatlab Ana Paula Del Vesco Eliane Gasparino Adhemar Rodrigues de Oliveira Neto |
| author_facet | Angélica de Souza Khatlab Ana Paula Del Vesco Eliane Gasparino Adhemar Rodrigues de Oliveira Neto |
| author_sort | Angélica de Souza Khatlab |
| collection | DOAJ |
| description | Two experiments were conducted to assess gender (Experiment 1) and age (Experiment 2) effects on the expression of genes related to lipid metabolism in broiler chickens. The expression of fatty acid synthase (FAS), apolipoprotein A-I (APOA-I), apolipoprotein B (APOB), adiponectin (ADIPOQ), liver kinase B1 (LKB1), and AMP-activated protein kinase α-1 (AMPKα-1) genes was evaluated by qRT-PCR. In Experiment 1, we observed a gender effect on feed intake, as male broilers presented greater feed intake than females. Female broilers presented greater gene expression of FAS, and lower expression of ADIPOQ and AMPKα-1, than males. A gender effect was not observed for the gene expression of APOA-I, APOB, or LKB1. In Experiment 2, there was a significant age effect on feed intake and weight gain. Broilers 42 days of age presented greater feed intake and weight gain than 21-day-old birds. 21-day-old broilers showed greater expression of APOA-I, ADIPOQ, LKB1, and AMPKα-1, and lower APOB gene expression in the liver than 42-day-old broilers. Age had no effect on FAS gene expression. Our results show that the gender and age could act on the expression of genes related to lipid synthesis, such as FAS and APOB, and also on genes related to lipid oxidation, such as ADIPOQ, LKB1, and AMPK. |
| first_indexed | 2024-04-10T08:25:02Z |
| format | Article |
| id | doaj.art-5eb1884ad7984817be5a0d0ce7f33f45 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:25:02Z |
| publishDate | 2018-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-5eb1884ad7984817be5a0d0ce7f33f452023-02-23T03:33:35ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092018-03-0163310310910.17221/41/2017-CJAScjs-201803-0003Gender and age effects on the expression of genes related to lipid metabolism in broiler's liverAngélica de Souza Khatlab0Ana Paula Del Vesco1Eliane Gasparino2Adhemar Rodrigues de Oliveira Neto3Department of Animal Science, State University of Maringá, Maringá, BrazilDepartment of Animal Science, Federal University of Sergipe, São Cristóvão, BrazilDepartment of Animal Science, State University of Maringá, Maringá, BrazilEVONIK of Brazil, São Paulo, BrazilTwo experiments were conducted to assess gender (Experiment 1) and age (Experiment 2) effects on the expression of genes related to lipid metabolism in broiler chickens. The expression of fatty acid synthase (FAS), apolipoprotein A-I (APOA-I), apolipoprotein B (APOB), adiponectin (ADIPOQ), liver kinase B1 (LKB1), and AMP-activated protein kinase α-1 (AMPKα-1) genes was evaluated by qRT-PCR. In Experiment 1, we observed a gender effect on feed intake, as male broilers presented greater feed intake than females. Female broilers presented greater gene expression of FAS, and lower expression of ADIPOQ and AMPKα-1, than males. A gender effect was not observed for the gene expression of APOA-I, APOB, or LKB1. In Experiment 2, there was a significant age effect on feed intake and weight gain. Broilers 42 days of age presented greater feed intake and weight gain than 21-day-old birds. 21-day-old broilers showed greater expression of APOA-I, ADIPOQ, LKB1, and AMPKα-1, and lower APOB gene expression in the liver than 42-day-old broilers. Age had no effect on FAS gene expression. Our results show that the gender and age could act on the expression of genes related to lipid synthesis, such as FAS and APOB, and also on genes related to lipid oxidation, such as ADIPOQ, LKB1, and AMPK.https://cjas.agriculturejournals.cz/artkey/cjs-201803-0003_gender-and-age-effects-on-the-expression-of-genes-related-to-lipid-metabolism-in-broiler-s-liver.phpapolipoproteinlipogenesislipolysis |
| spellingShingle | Angélica de Souza Khatlab Ana Paula Del Vesco Eliane Gasparino Adhemar Rodrigues de Oliveira Neto Gender and age effects on the expression of genes related to lipid metabolism in broiler's liver Czech Journal of Animal Science apolipoprotein lipogenesis lipolysis |
| title | Gender and age effects on the expression of genes related to lipid metabolism in broiler's liver |
| title_full | Gender and age effects on the expression of genes related to lipid metabolism in broiler's liver |
| title_fullStr | Gender and age effects on the expression of genes related to lipid metabolism in broiler's liver |
| title_full_unstemmed | Gender and age effects on the expression of genes related to lipid metabolism in broiler's liver |
| title_short | Gender and age effects on the expression of genes related to lipid metabolism in broiler's liver |
| title_sort | gender and age effects on the expression of genes related to lipid metabolism in broiler s liver |
| topic | apolipoprotein lipogenesis lipolysis |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201803-0003_gender-and-age-effects-on-the-expression-of-genes-related-to-lipid-metabolism-in-broiler-s-liver.php |
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