Electroporation of embryonic chick skin explant
Since the introduction of electroporation a decade ago, the chick embryo has evolved into a powerful system for studying gene regulation and function during development. Although this is a straightforward method for embryos, skin (especially lamellar tissue) present special challenges. Here we descr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | Medicine in Novel Technology and Devices |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590093522000819 |
| _version_ | 1811291628752601088 |
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| author | Tailin Chen Wanjuan Bu Jun Guo Yuxiao Zhang Min Tan Jing Du Yubo Fan |
| author_facet | Tailin Chen Wanjuan Bu Jun Guo Yuxiao Zhang Min Tan Jing Du Yubo Fan |
| author_sort | Tailin Chen |
| collection | DOAJ |
| description | Since the introduction of electroporation a decade ago, the chick embryo has evolved into a powerful system for studying gene regulation and function during development. Although this is a straightforward method for embryos, skin (especially lamellar tissue) present special challenges. Here we describe a protocol for electroporation of expression vectors into the chick skin explant, with precise spatial and temporal control. We have improved the design of the electroporation chamber, which can achieve precise regulation of the electroporation area by changing the parameters of the laser cutting of the acrylic plate. We give the electroporation parameters of chicken embryo skin, which can effectively improve the electroporation efficiency. This technique can be used for time-lapse imaging and to study gene regulation in chick embryo skin. |
| first_indexed | 2024-04-13T04:33:12Z |
| format | Article |
| id | doaj.art-5eb36eb2268a42a3bb5dc307475d2a34 |
| institution | Directory Open Access Journal |
| issn | 2590-0935 |
| language | English |
| last_indexed | 2024-04-13T04:33:12Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Medicine in Novel Technology and Devices |
| spelling | doaj.art-5eb36eb2268a42a3bb5dc307475d2a342022-12-22T03:02:16ZengElsevierMedicine in Novel Technology and Devices2590-09352022-12-0116100194Electroporation of embryonic chick skin explantTailin Chen0Wanjuan Bu1Jun Guo2Yuxiao Zhang3Min Tan4Jing Du5Yubo Fan6Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, ChinaKey Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, ChinaKey Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, ChinaKey Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, ChinaKey Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, ChinaCorresponding author.; Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, ChinaCorresponding author.; Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, ChinaSince the introduction of electroporation a decade ago, the chick embryo has evolved into a powerful system for studying gene regulation and function during development. Although this is a straightforward method for embryos, skin (especially lamellar tissue) present special challenges. Here we describe a protocol for electroporation of expression vectors into the chick skin explant, with precise spatial and temporal control. We have improved the design of the electroporation chamber, which can achieve precise regulation of the electroporation area by changing the parameters of the laser cutting of the acrylic plate. We give the electroporation parameters of chicken embryo skin, which can effectively improve the electroporation efficiency. This technique can be used for time-lapse imaging and to study gene regulation in chick embryo skin.http://www.sciencedirect.com/science/article/pii/S2590093522000819ElectroporationEmbryonic chick skin explantElectroporation chamber |
| spellingShingle | Tailin Chen Wanjuan Bu Jun Guo Yuxiao Zhang Min Tan Jing Du Yubo Fan Electroporation of embryonic chick skin explant Medicine in Novel Technology and Devices Electroporation Embryonic chick skin explant Electroporation chamber |
| title | Electroporation of embryonic chick skin explant |
| title_full | Electroporation of embryonic chick skin explant |
| title_fullStr | Electroporation of embryonic chick skin explant |
| title_full_unstemmed | Electroporation of embryonic chick skin explant |
| title_short | Electroporation of embryonic chick skin explant |
| title_sort | electroporation of embryonic chick skin explant |
| topic | Electroporation Embryonic chick skin explant Electroporation chamber |
| url | http://www.sciencedirect.com/science/article/pii/S2590093522000819 |
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