Impedance In Vitro Assessment for the Detection of <i>Salmonella typhimurium</i> Infection in Intestinal Human Cancer Cells
A significant number of research papers regarding biosensor-related assays for key food safety pathogens based on the use of mammalian cells has been reported. In this study, the <i>Salmonella typhimurium</i> infection progression was monitored in the human colon cancer cell line Caco-2...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/11/10/534 |
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| author | Sofia Marka Maria-Eleftheria Zografaki George-Marios Papaioannou Sofia Mavrikou Emmanouil Flemetakis Spyridon Kintzios |
| author_facet | Sofia Marka Maria-Eleftheria Zografaki George-Marios Papaioannou Sofia Mavrikou Emmanouil Flemetakis Spyridon Kintzios |
| author_sort | Sofia Marka |
| collection | DOAJ |
| description | A significant number of research papers regarding biosensor-related assays for key food safety pathogens based on the use of mammalian cells has been reported. In this study, the <i>Salmonella typhimurium</i> infection progression was monitored in the human colon cancer cell line Caco-2 and the mucus-secreting HT29-MTX-E12, after treatment with five different bacterial MOI for 30 min by comparing the alterations of frequencies recordings with impedance spectroscopy measurements. For this purpose, bacterial adhesion and invasion assays were initially performed. Then, the data obtained from impedance spectroscopy recordings were compared to cell viability data derived from the MTT uptake cell proliferation assay as well as from live cell analysis assays of mitochondrial membrane potential alterations. From our findings a concentration-dependent increase in bacterial colonies occurring from invaded cells was observed upon a higher multiplicity of infection (MOI) bacterial infection at both cell lines. On the contrary, the bacteria infection did not have any impact on the viability of the cells after 1 h of treatment. Differential results were obtained from the measurement of mitochondrial potential at both cell lines. Finally, the impedance values recorded from the 2D, and 3D cultures were concentration-dependent for both cell lines whereas a characteristic pattern specific to each cell line was revealed. Our results indicate that human cell-based bio-electric assays can be a valuable tool for obtaining a unique fingerprint for each bacterial infection in the near future. |
| first_indexed | 2024-03-10T21:20:36Z |
| format | Article |
| id | doaj.art-5eb40c0b27ad4131ba701a1a23281362 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T21:20:36Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-5eb40c0b27ad4131ba701a1a232813622023-11-19T16:03:56ZengMDPI AGChemosensors2227-90402023-10-01111053410.3390/chemosensors11100534Impedance In Vitro Assessment for the Detection of <i>Salmonella typhimurium</i> Infection in Intestinal Human Cancer CellsSofia Marka0Maria-Eleftheria Zografaki1George-Marios Papaioannou2Sofia Mavrikou3Emmanouil Flemetakis4Spyridon Kintzios5Laboratory of Cell Technology, Department of Biotechnology, Agricultural University of Athens, EU-CONEXUS European University, 11855 Athens, GreeceLaboratory of Molecular Biology, Department of Biotechnology, Agricultural University of Athens, EU-CONEXUS European University, 11855 Athens, GreeceLaboratory of Cell Technology, Department of Biotechnology, Agricultural University of Athens, EU-CONEXUS European University, 11855 Athens, GreeceLaboratory of Cell Technology, Department of Biotechnology, Agricultural University of Athens, EU-CONEXUS European University, 11855 Athens, GreeceLaboratory of Molecular Biology, Department of Biotechnology, Agricultural University of Athens, EU-CONEXUS European University, 11855 Athens, GreeceLaboratory of Cell Technology, Department of Biotechnology, Agricultural University of Athens, EU-CONEXUS European University, 11855 Athens, GreeceA significant number of research papers regarding biosensor-related assays for key food safety pathogens based on the use of mammalian cells has been reported. In this study, the <i>Salmonella typhimurium</i> infection progression was monitored in the human colon cancer cell line Caco-2 and the mucus-secreting HT29-MTX-E12, after treatment with five different bacterial MOI for 30 min by comparing the alterations of frequencies recordings with impedance spectroscopy measurements. For this purpose, bacterial adhesion and invasion assays were initially performed. Then, the data obtained from impedance spectroscopy recordings were compared to cell viability data derived from the MTT uptake cell proliferation assay as well as from live cell analysis assays of mitochondrial membrane potential alterations. From our findings a concentration-dependent increase in bacterial colonies occurring from invaded cells was observed upon a higher multiplicity of infection (MOI) bacterial infection at both cell lines. On the contrary, the bacteria infection did not have any impact on the viability of the cells after 1 h of treatment. Differential results were obtained from the measurement of mitochondrial potential at both cell lines. Finally, the impedance values recorded from the 2D, and 3D cultures were concentration-dependent for both cell lines whereas a characteristic pattern specific to each cell line was revealed. Our results indicate that human cell-based bio-electric assays can be a valuable tool for obtaining a unique fingerprint for each bacterial infection in the near future.https://www.mdpi.com/2227-9040/11/10/534Caco-2HT29-MTX-E12impedance spectroscopyinfection progression monitoringscreen printed electrodes |
| spellingShingle | Sofia Marka Maria-Eleftheria Zografaki George-Marios Papaioannou Sofia Mavrikou Emmanouil Flemetakis Spyridon Kintzios Impedance In Vitro Assessment for the Detection of <i>Salmonella typhimurium</i> Infection in Intestinal Human Cancer Cells Chemosensors Caco-2 HT29-MTX-E12 impedance spectroscopy infection progression monitoring screen printed electrodes |
| title | Impedance In Vitro Assessment for the Detection of <i>Salmonella typhimurium</i> Infection in Intestinal Human Cancer Cells |
| title_full | Impedance In Vitro Assessment for the Detection of <i>Salmonella typhimurium</i> Infection in Intestinal Human Cancer Cells |
| title_fullStr | Impedance In Vitro Assessment for the Detection of <i>Salmonella typhimurium</i> Infection in Intestinal Human Cancer Cells |
| title_full_unstemmed | Impedance In Vitro Assessment for the Detection of <i>Salmonella typhimurium</i> Infection in Intestinal Human Cancer Cells |
| title_short | Impedance In Vitro Assessment for the Detection of <i>Salmonella typhimurium</i> Infection in Intestinal Human Cancer Cells |
| title_sort | impedance in vitro assessment for the detection of i salmonella typhimurium i infection in intestinal human cancer cells |
| topic | Caco-2 HT29-MTX-E12 impedance spectroscopy infection progression monitoring screen printed electrodes |
| url | https://www.mdpi.com/2227-9040/11/10/534 |
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